Detection of protein concentrations using a pH-step titration method

J. Kruise; J.C.T. Eijkel; P. Bergveld

doi:10.1016/S0925-4005(97)00221-9

Detection of protein concentrations using a pH-step titration method

J. Kruise, J.C.T. Eijkel, P. Bergveld

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Abstract

A stimulus-response method based on the application of a pH step is proposed for the detection of protein immobilized in a membrane on top of an ion-sensitive field-effect transistor (ISFET). The ISFET response to a step-wise change in pH, applied at the interface between the membrane and the surrounding solution, depends on the concentration of protein immobilized in the membrane because proton-dissociation reactions of the protein cause a delayed diffusion of protons and hydroxyl ions through the membrane. Our theoretical description shows that the delay in ISFET response is linearly related to the concentration of protein immobilized in the membrane. Experiments performed with lysozyme as a model protein show the feasibility of this detection principle.

Original language	English
Pages (from-to)	297-303
Number of pages	7
Journal	Sensors and Actuators B: Chemical
Volume	44
Issue number	1-3
DOIs	https://doi.org/10.1016/S0925-4005(97)00221-9
Publication status	Published - 1997

Keywords

Protein
Ion-sensitive field effect transistor (ISFET)
pH-step titration

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10.1016/S0925-4005(97)00221-9

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abstract = "A stimulus-response method based on the application of a pH step is proposed for the detection of protein immobilized in a membrane on top of an ion-sensitive field-effect transistor (ISFET). The ISFET response to a step-wise change in pH, applied at the interface between the membrane and the surrounding solution, depends on the concentration of protein immobilized in the membrane because proton-dissociation reactions of the protein cause a delayed diffusion of protons and hydroxyl ions through the membrane. Our theoretical description shows that the delay in ISFET response is linearly related to the concentration of protein immobilized in the membrane. Experiments performed with lysozyme as a model protein show the feasibility of this detection principle.",

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AU - Kruise, J.

AU - Eijkel, J.C.T.

AU - Bergveld, P.

PY - 1997

Y1 - 1997

N2 - A stimulus-response method based on the application of a pH step is proposed for the detection of protein immobilized in a membrane on top of an ion-sensitive field-effect transistor (ISFET). The ISFET response to a step-wise change in pH, applied at the interface between the membrane and the surrounding solution, depends on the concentration of protein immobilized in the membrane because proton-dissociation reactions of the protein cause a delayed diffusion of protons and hydroxyl ions through the membrane. Our theoretical description shows that the delay in ISFET response is linearly related to the concentration of protein immobilized in the membrane. Experiments performed with lysozyme as a model protein show the feasibility of this detection principle.

AB - A stimulus-response method based on the application of a pH step is proposed for the detection of protein immobilized in a membrane on top of an ion-sensitive field-effect transistor (ISFET). The ISFET response to a step-wise change in pH, applied at the interface between the membrane and the surrounding solution, depends on the concentration of protein immobilized in the membrane because proton-dissociation reactions of the protein cause a delayed diffusion of protons and hydroxyl ions through the membrane. Our theoretical description shows that the delay in ISFET response is linearly related to the concentration of protein immobilized in the membrane. Experiments performed with lysozyme as a model protein show the feasibility of this detection principle.

KW - Protein

KW - Ion-sensitive field effect transistor (ISFET)

KW - pH-step titration

U2 - 10.1016/S0925-4005(97)00221-9

DO - 10.1016/S0925-4005(97)00221-9

M3 - Article

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JO - Sensors and Actuators B: Chemical

JF - Sensors and Actuators B: Chemical

IS - 1-3

ER -

Detection of protein concentrations using a pH-step titration method

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Keywords

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