Determination of aldehydes and ketones using derivatization with 2,4-dinitrophenylhydrazine (DNPH) and liquid chromatography/atmospheric pressure photoionization-mass spectrometry (LC/APPI-MS)

Suze M. van Leeuwen, Laurens Hendriksen, Uwe Karst

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Abstract

Atmospheric pressure photoionization-mass spectrometry (APPI-MS) is used for the analysis of aldehydes and ketones after derivatization with 2,4-dinitrophenylhydrazine (DNPH) and liquid chromatographic separation. In the negative ion mode, the [M − H]− pseudomolecular ions are most abundant for the carbonyls. Compared with the established atmospheric pressure chemical ionization (APCI)-MS, limits of detection are typically lower using similar conditions. Automobile exhaust and cigarette exhaust samples were analyzed with APPI-MS and APCI-MS in combination with an ion trap mass analyzer. Due to improved limits of detection, more of the less abundant long-chain carbonyls are detected with APPI-MS in real samples. While 2,4-dinitrophenylazide, a known reaction product of DNPH with nitrogen dioxide, is detected in APCI-MS due to dissociative electron capture, it is not observed at all in APPI-MS.
Original languageEnglish
Pages (from-to)107-112
Number of pages6
JournalJournal of chromatography A
Volume1058
Issue number1-2
DOIs
Publication statusPublished - 2004

Keywords

  • 2
  • Carbonyls
  • Atmospheric pressure photoionization
  • METIS-222355
  • IR-76287
  • 4-Dinitrophenylhydrazine

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