Determination of binding constants of polyethylene glycol vancomycin derivatives to peptide ligands using affinity capillary electrophoresis

L. Hernandez, M. Rudolph, R. Lammertink, J. Kornfield, C. Zurita, F. A. Gomez*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

5 Citations (Scopus)

Abstract

Vancomycin (Van) from Streptomyces orientalis has been derivatized with polyethylene glycol [PEG; PEG-550 (1), 750 (2), 1,100 (3), 2,000 (4), 5,000 (5), and 8,000 (6) g mol-1] at the N-terminus of the glycopeptide backbone and their binding to d-Ala-d-Ala terminus peptides assessed using affinity capillary electrophoresis (ACE). Utilizing ACE, a plug of Van-PEG and non-interacting standards are injected and electrophoresed. Analysis of the change in the relative migration time ratio of the Van-PEG species, relative to the non-interacting standards, as a function of the concentration of peptide, yields a value for the binding constant (K b). Values of K b for N-acetyl-d-Ala-d-Ala, 7 to the Van-PEG derivatives are weaker than those for N α,N ε-diacetyl-Lys-d-Ala-d- Ala, 8 (for example, values of K b for 7-1 and 8-1 are 1.8 and 47.7 × 103M-1, respectively). These results demonstrate that derivatization of Van with PEG has little effect on the affinity of d-Ala-d-Ala peptide ligands to it. The findings further prove the versatility of ACE and its ability to estimate binding parameters of ligands to antibiotics.

Original languageEnglish
Pages (from-to)299-303
Number of pages5
JournalChromatographia
Volume65
Issue number5-6
DOIs
Publication statusPublished - 1 Mar 2007
Externally publishedYes

Keywords

  • Affinity capillary electrophoresis
  • Binding constants
  • Polyethylene glycol
  • Vancomycin

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