Determination of telmisartan in human blood plasma: Part I: Immunoassay development

C.M. Hempen; Liane Gläsle-Schwarz; Ulrich Kunz; U. Karst

doi:10.1016/j.aca.2005.12.036

Determination of telmisartan in human blood plasma: Part I: Immunoassay development

C.M. Hempen, Liane Gläsle-Schwarz, Ulrich Kunz, U. Karst

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Abstract

Telmisartan is an angiotensin II receptor antagonist and a known drug against high blood pressure. In this report, the development of a new and rapid analytical technique, an enzyme-linked immunosorbent assay (ELISA) for the determination of telmisartan in human blood plasma is described. The immunoassay is based on a conversion of 4-(N-methylhydrazino)-7-nitro-2,1,3-benzooxadiazole (MNBDH) to 4-(N-methylamino)-7-nitro-2,1,3-benzooxadiazole (MNBDA), which is detected by fluorescence spectroscopy. The limit of detection was 0.1 ng/mL, the limit of quantification was 0.3 ng/mL and the working range extended from 0.3 ng/mL to 300 ng/mL.

Original language	Undefined
Pages (from-to)	35-40
Journal	Analytica chimica acta
Volume	560
Issue number	1-2
DOIs	https://doi.org/10.1016/j.aca.2005.12.036
Publication status	Published - 2006

Keywords

IR-74291

Access to Document

10.1016/j.aca.2005.12.036

Cite this

Hempen, C. M., Gläsle-Schwarz, L., Kunz, U., & Karst, U. (2006). Determination of telmisartan in human blood plasma: Part I: Immunoassay development. Analytica chimica acta, 560(1-2), 35-40. https://doi.org/10.1016/j.aca.2005.12.036

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AU - Kunz, Ulrich

AU - Karst, U.

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Y1 - 2006

N2 - Telmisartan is an angiotensin II receptor antagonist and a known drug against high blood pressure. In this report, the development of a new and rapid analytical technique, an enzyme-linked immunosorbent assay (ELISA) for the determination of telmisartan in human blood plasma is described. The immunoassay is based on a conversion of 4-(N-methylhydrazino)-7-nitro-2,1,3-benzooxadiazole (MNBDH) to 4-(N-methylamino)-7-nitro-2,1,3-benzooxadiazole (MNBDA), which is detected by fluorescence spectroscopy. The limit of detection was 0.1 ng/mL, the limit of quantification was 0.3 ng/mL and the working range extended from 0.3 ng/mL to 300 ng/mL.

AB - Telmisartan is an angiotensin II receptor antagonist and a known drug against high blood pressure. In this report, the development of a new and rapid analytical technique, an enzyme-linked immunosorbent assay (ELISA) for the determination of telmisartan in human blood plasma is described. The immunoassay is based on a conversion of 4-(N-methylhydrazino)-7-nitro-2,1,3-benzooxadiazole (MNBDH) to 4-(N-methylamino)-7-nitro-2,1,3-benzooxadiazole (MNBDA), which is detected by fluorescence spectroscopy. The limit of detection was 0.1 ng/mL, the limit of quantification was 0.3 ng/mL and the working range extended from 0.3 ng/mL to 300 ng/mL.

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DO - 10.1016/j.aca.2005.12.036

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