TY - JOUR
T1 - Determining Particle Size and Position in a Coplanar Electrode Setup Using Measured Opacity for Microfluidic Cytometry
AU - de Bruijn, Douwe S.
AU - Jorissen, Koen F.A.
AU - Olthuis, Wouter
AU - van den Berg, Albert
N1 - Funding Information:
Funding: This work is part of the research program of the Foundation for Fundamental Research on Matter (FOM), which is part of the Dutch Research Council (NWO).
Funding Information:
Acknowledgments: We would like to thank the Max Planck—Center for Complex Fluid Dynamics for support of this project.
Publisher Copyright:
© 2021 by the authors.
Financial transaction number:
342142622
PY - 2021/9/23
Y1 - 2021/9/23
N2 - Microfluidic impedance flow cytometers enable high-throughput, non-invasive, and label-free detection of single-cells. Cytometers with coplanar electrodes are easy and cheap to fabricate, but are sensitive to positional differences of passing particles, owing to the inhomogeneous electric field. We present a novel particle height compensation method, which employs the dependence of measured electrical opacity on particle height. The measured electrical opacity correlates with the particle height as a result of the constant electrical double layer series capacitance of the electrodes. As an alternative to existing compensation methods, we use only two coplanar electrodes and multi-frequency analysis to determine the particle size of a mixture of 5, 6, and 7 µm polystyrene beads with an accuracy (CV) of 5.8%, 4.0%, and 2.9%, respectively. Additionally, we can predict the bead height with an accuracy of 1.5 µm (8% of channel height) using the measured opacity and we demonstrate its application in flow cytometry with yeast. The use of only two electrodes is of special interest for simplified, easy-to-use chips with a minimum amount of instrumentation and of limited size.
AB - Microfluidic impedance flow cytometers enable high-throughput, non-invasive, and label-free detection of single-cells. Cytometers with coplanar electrodes are easy and cheap to fabricate, but are sensitive to positional differences of passing particles, owing to the inhomogeneous electric field. We present a novel particle height compensation method, which employs the dependence of measured electrical opacity on particle height. The measured electrical opacity correlates with the particle height as a result of the constant electrical double layer series capacitance of the electrodes. As an alternative to existing compensation methods, we use only two coplanar electrodes and multi-frequency analysis to determine the particle size of a mixture of 5, 6, and 7 µm polystyrene beads with an accuracy (CV) of 5.8%, 4.0%, and 2.9%, respectively. Additionally, we can predict the bead height with an accuracy of 1.5 µm (8% of channel height) using the measured opacity and we demonstrate its application in flow cytometry with yeast. The use of only two electrodes is of special interest for simplified, easy-to-use chips with a minimum amount of instrumentation and of limited size.
KW - Coplanar electrodes
KW - Electrical opacity
KW - Impedance cytometry
KW - Microfluidics
KW - Particle tracking
KW - Positional dependence
UR - http://www.scopus.com/inward/record.url?scp=85116933821&partnerID=8YFLogxK
U2 - 10.3390/bios11100353
DO - 10.3390/bios11100353
M3 - Article
AN - SCOPUS:85116933821
SN - 2079-6374
VL - 11
JO - Biosensors
JF - Biosensors
IS - 10
M1 - 353
ER -