Developing an amperometric hydrogen peroxide sensor for an exhaled breath analysis system

Justyna Wiedemair, Henriëtte van Dorp, Wouter Olthuis, Albert van den Berg

    Research output: Contribution to journalArticleAcademicpeer-review

    14 Citations (Scopus)

    Abstract

    In this work, we present a chip-integrated amperometric sensor targeted at the detection of hydrogen peroxide (H2O2) in the gaseous phase. Electrode chips are manufactured in a series of microfabrication steps and characterized electrochemically. Using such devices detection of H2O2 in an aqueous phase is shown by means of cyclic voltammetry and amperometry. Furthermore, it is discussed that variation of conditions such as the composition of the supporting electrolyte largely influences the obtained electrochemical signal. Additionally, electrochemical pretreatment of platinum working electrodes aiming at surface oxidation improves the limit of detection of the sensor and the linearity of the calibration curve at low H2O2 concentrations (<10 mu M). Agarose-coated electrode chips are used for the measurement of H2O2 in the gaseous phase. Detection of H2O2 is shown in a static and in a flow-through setup. We find a limit of detection of approximately 42 ppb. Current work focuses on expanding the presented device to detection of H2O2 in exhaled breath condensate.
    Original languageUndefined
    Pages (from-to)3181-3186
    Number of pages6
    JournalElectrophoresis
    Volume33
    Issue number21
    DOIs
    Publication statusPublished - Nov 2012

    Keywords

    • METIS-293243
    • EWI-22681
    • IR-83527

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