Development and characterization of poly(ε-caprolactone) hollow fiber membranes for vascular tissue engineering

Nazely Diban-Ibrahim Gomez, Suvi Haimi, Lydia A.M. Bolhuis-Versteeg, Sandra Da Silva Teixeira, S. Miettinen, Andreas A. Poot, Dirk W. Grijpma, Dimitrios Stamatialis

Research output: Contribution to journalArticleAcademicpeer-review

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Abstract

The fabrication of tissue-engineered scaffolds for small-caliber blood vessels still remains a challenge. In the present work, we prepared poly(ε-caprolactone) (PCL) hollow fiber (HF) membranes, suitable for small-diameter blood vessel regeneration, by a phase separation spinning technique. The difficulty of processing PCL, a highly elastic material prone to suffer die swelling by extrusion, was overcome by tailoring the dope solution temperature and extrusion flow rate during the spinning procedure. The influence of the composition of the coagulation bath (water, ethanol, isopropanol) on the HF membrane physico-chemical properties (morphology, transport and mechanical properties) and cell attachment and proliferation was studied. The HF membranes fabricated using ethanol as coagulation bath had the most uniform morphology, good mechanical and transport properties and showed human adipose stem cell attachment and proliferation. Therefore, these fibers are promising scaffolds for small-caliber blood vessel regeneration.
Original languageUndefined
Pages (from-to)29-37
JournalJournal of membrane science
Volume438
DOIs
Publication statusPublished - 2013

Keywords

  • METIS-301773
  • IR-90150

Cite this

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title = "Development and characterization of poly(ε-caprolactone) hollow fiber membranes for vascular tissue engineering",
abstract = "The fabrication of tissue-engineered scaffolds for small-caliber blood vessels still remains a challenge. In the present work, we prepared poly(ε-caprolactone) (PCL) hollow fiber (HF) membranes, suitable for small-diameter blood vessel regeneration, by a phase separation spinning technique. The difficulty of processing PCL, a highly elastic material prone to suffer die swelling by extrusion, was overcome by tailoring the dope solution temperature and extrusion flow rate during the spinning procedure. The influence of the composition of the coagulation bath (water, ethanol, isopropanol) on the HF membrane physico-chemical properties (morphology, transport and mechanical properties) and cell attachment and proliferation was studied. The HF membranes fabricated using ethanol as coagulation bath had the most uniform morphology, good mechanical and transport properties and showed human adipose stem cell attachment and proliferation. Therefore, these fibers are promising scaffolds for small-caliber blood vessel regeneration.",
keywords = "METIS-301773, IR-90150",
author = "{Diban-Ibrahim Gomez}, Nazely and Suvi Haimi and Bolhuis-Versteeg, {Lydia A.M.} and {Da Silva Teixeira}, Sandra and S. Miettinen and Poot, {Andreas A.} and Grijpma, {Dirk W.} and Dimitrios Stamatialis",
year = "2013",
doi = "10.1016/j.memsci.2013.03.024",
language = "Undefined",
volume = "438",
pages = "29--37",
journal = "Journal of membrane science",
issn = "0376-7388",
publisher = "Elsevier",

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Development and characterization of poly(ε-caprolactone) hollow fiber membranes for vascular tissue engineering. / Diban-Ibrahim Gomez, Nazely; Haimi, Suvi; Bolhuis-Versteeg, Lydia A.M.; Da Silva Teixeira, Sandra; Miettinen, S.; Poot, Andreas A.; Grijpma, Dirk W.; Stamatialis, Dimitrios.

In: Journal of membrane science, Vol. 438, 2013, p. 29-37.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Development and characterization of poly(ε-caprolactone) hollow fiber membranes for vascular tissue engineering

AU - Diban-Ibrahim Gomez, Nazely

AU - Haimi, Suvi

AU - Bolhuis-Versteeg, Lydia A.M.

AU - Da Silva Teixeira, Sandra

AU - Miettinen, S.

AU - Poot, Andreas A.

AU - Grijpma, Dirk W.

AU - Stamatialis, Dimitrios

PY - 2013

Y1 - 2013

N2 - The fabrication of tissue-engineered scaffolds for small-caliber blood vessels still remains a challenge. In the present work, we prepared poly(ε-caprolactone) (PCL) hollow fiber (HF) membranes, suitable for small-diameter blood vessel regeneration, by a phase separation spinning technique. The difficulty of processing PCL, a highly elastic material prone to suffer die swelling by extrusion, was overcome by tailoring the dope solution temperature and extrusion flow rate during the spinning procedure. The influence of the composition of the coagulation bath (water, ethanol, isopropanol) on the HF membrane physico-chemical properties (morphology, transport and mechanical properties) and cell attachment and proliferation was studied. The HF membranes fabricated using ethanol as coagulation bath had the most uniform morphology, good mechanical and transport properties and showed human adipose stem cell attachment and proliferation. Therefore, these fibers are promising scaffolds for small-caliber blood vessel regeneration.

AB - The fabrication of tissue-engineered scaffolds for small-caliber blood vessels still remains a challenge. In the present work, we prepared poly(ε-caprolactone) (PCL) hollow fiber (HF) membranes, suitable for small-diameter blood vessel regeneration, by a phase separation spinning technique. The difficulty of processing PCL, a highly elastic material prone to suffer die swelling by extrusion, was overcome by tailoring the dope solution temperature and extrusion flow rate during the spinning procedure. The influence of the composition of the coagulation bath (water, ethanol, isopropanol) on the HF membrane physico-chemical properties (morphology, transport and mechanical properties) and cell attachment and proliferation was studied. The HF membranes fabricated using ethanol as coagulation bath had the most uniform morphology, good mechanical and transport properties and showed human adipose stem cell attachment and proliferation. Therefore, these fibers are promising scaffolds for small-caliber blood vessel regeneration.

KW - METIS-301773

KW - IR-90150

U2 - 10.1016/j.memsci.2013.03.024

DO - 10.1016/j.memsci.2013.03.024

M3 - Article

VL - 438

SP - 29

EP - 37

JO - Journal of membrane science

JF - Journal of membrane science

SN - 0376-7388

ER -