TY - JOUR
T1 - Development of double porous poly (ε-caprolactone)/chitosan polymer as tissue engineering scaffold
AU - Das, Pritam
AU - Remigy, Jean Christophe
AU - Lahitte, Jean François
AU - van der Meer, Andries D.
AU - Garmy-Susini, Barbara
AU - Coetsier, Clémence
AU - Desclaux, Sandrine
AU - Bacchin, Patrice
PY - 2020/2/1
Y1 - 2020/2/1
N2 - Polymer blend made from poly(ε - caprolactone)/chitosan (PCL/CHT) offers interesting opportunities for biological applications. The paper presents a new way to fabricate PCL/CHT double-porosity (macrovoids with interconnected microporosity) membrane materials from a chemical optimization of the solvent and non-solvent phases and from a modified phase inversion technique. By varying the PCL/CHT proportion, it is shown that it is possible to improve the chemical and physical properties of the CHT carbohydrate polymer. The PCL/CHT membranes are fully characterized in term of physico-chemical properties (ATR-FTIR, XRD and DSC) to understand the miscibility of the two-polymer blend. Morphological characterization by SEM shows that by increasing CHT wt% in the blend, the size of the macrovoids was increasing. Rapid enzymatic degradation of PCL from all the blend was found by using lipase (from P. cepacia). The mechanisms at the origin of the morphological structuration of the material is also discussed. To test the ability to operate these materials as small diameter vascular scaffolds, cell culture with human umbilical vein endothelial cells (HUVECs) were carried out on the membrane and the results analyzed with laser scanning confocal microscopy (LSCM). Data suggest that the blend membrane with higher concentration of CHT polymer wt% have suitable properties that promote high number of cells on the surface by maintaining cellular cytoskeleton integrity within 3 days. The blend membrane with a double porous morphology could be potentially applicable in future for small diameter vascular graft application. The surface macrovoids (20–90 μm) could be useful for three-dimensional cellular adhesion and proliferation and interconnected microporous spongy network (7–20 μm) is expected to transfer essential nutrients, oxygen, growth factor between the macrovoids and the supernatant.
AB - Polymer blend made from poly(ε - caprolactone)/chitosan (PCL/CHT) offers interesting opportunities for biological applications. The paper presents a new way to fabricate PCL/CHT double-porosity (macrovoids with interconnected microporosity) membrane materials from a chemical optimization of the solvent and non-solvent phases and from a modified phase inversion technique. By varying the PCL/CHT proportion, it is shown that it is possible to improve the chemical and physical properties of the CHT carbohydrate polymer. The PCL/CHT membranes are fully characterized in term of physico-chemical properties (ATR-FTIR, XRD and DSC) to understand the miscibility of the two-polymer blend. Morphological characterization by SEM shows that by increasing CHT wt% in the blend, the size of the macrovoids was increasing. Rapid enzymatic degradation of PCL from all the blend was found by using lipase (from P. cepacia). The mechanisms at the origin of the morphological structuration of the material is also discussed. To test the ability to operate these materials as small diameter vascular scaffolds, cell culture with human umbilical vein endothelial cells (HUVECs) were carried out on the membrane and the results analyzed with laser scanning confocal microscopy (LSCM). Data suggest that the blend membrane with higher concentration of CHT polymer wt% have suitable properties that promote high number of cells on the surface by maintaining cellular cytoskeleton integrity within 3 days. The blend membrane with a double porous morphology could be potentially applicable in future for small diameter vascular graft application. The surface macrovoids (20–90 μm) could be useful for three-dimensional cellular adhesion and proliferation and interconnected microporous spongy network (7–20 μm) is expected to transfer essential nutrients, oxygen, growth factor between the macrovoids and the supernatant.
KW - Cell culture
KW - Chitosan
KW - Double porous
KW - Membrane
KW - Poly (ε -caprolactone)
KW - Biocompatibility
KW - Poly (epsilon -caprolactone)
KW - 22/2 OA procedure
UR - http://www.scopus.com/inward/record.url?scp=85073723686&partnerID=8YFLogxK
U2 - 10.1016/j.msec.2019.110257
DO - 10.1016/j.msec.2019.110257
M3 - Article
AN - SCOPUS:85073723686
SN - 0928-4931
VL - 107
JO - Materials Science and Engineering C: Materials for Biological Applications
JF - Materials Science and Engineering C: Materials for Biological Applications
M1 - 110257
ER -