Differential Labeling of Free and Disulfide-Bound Thiol Functions in Proteins

B. Seiwert, H. Hayen, U. Karst

Research output: Contribution to journalArticleAcademicpeer-review

19 Citations (Scopus)
44 Downloads (Pure)

Abstract

A method for the simultaneous determination of the number of free cysteine groups and disulfide-bound cysteine groups in proteins has been developed based on the sequential labeling of free and bound thiol functionalities with two ferrocene-based maleimide reagents. Liquid chromatography/electrochemistry/mass spectrometry was used to assign the N-(2-ferroceneethyl)maleimide (FEM) labeled free cysteine functionalities in a tryptic digest mixture, whereas a precursor ion scan enables the detection of peptides with ferrocenecarboxylic acid-(2-maleimidoyl)ethylamide (FMEA) labeled disulfide-bound cysteine groups after reduction. Fragment spectra of the labeled peptides yield an excellent coverage of b-type and y-type ions. The ferrocene labeled cysteines were fragmented as 412 Da (FEM) and 455 Da (FMEA). These fragment masses are significantly higher than unlabeled amino acids or dipeptides and are easily detected. The position of free and disulfide-bound cysteine may therefore be assigned in an amino acid sequence.
Original languageEnglish
Pages (from-to)1-7
JournalJournal of the American Society for Mass Spectrometry
Volume19
Issue number1
DOIs
Publication statusPublished - 2008

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