Differential SPR immunosensing

Charles E.H. Berger; C.E.H. Berger; Jan Greve

doi:10.1016/S0925-4005(00)00307-5

Differential SPR immunosensing

Charles E.H. Berger, C.E.H. Berger, Jan Greve

Research output: Contribution to journal › Article › Academic › peer-review

55 Citations (Scopus)

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Abstract

In this work we describe a surface plasmon resonance (SPR) sensor with a differential detection of the SPR angle, and demonstrate it. The angle of incidence is modulated by a simple piezo-electric actuator, and the reflectance signal is measured with a lockin-amplifier. When the conditions for SPR are fulfilled, the differential signal is zero. The shift of the resonance conditions can be measured as an increase of the differential signal, or using feedback on the angle of incidence. The sensor will be demonstrated by monitoring an adsorption and an immunoreaction at the sensor surface with sub-mdeg. resolution. The modulated incident beam can also be scanned past a number of sensing areas, making multichannel measurements possible, as will be demonstrated.

Original language	Undefined
Pages (from-to)	103-108
Number of pages	6
Journal	Sensors and Actuators B: Chemical
Volume	63
Issue number	1-2
DOIs	https://doi.org/10.1016/S0925-4005(00)00307-5
Publication status	Published - 2000

Keywords

Surface plasmon resonance
METIS-128419
Immunosensor
IR-74263

Access to Document

10.1016/S0925-4005(00)00307-5

Cite this

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title = "Differential SPR immunosensing",

abstract = "In this work we describe a surface plasmon resonance (SPR) sensor with a differential detection of the SPR angle, and demonstrate it. The angle of incidence is modulated by a simple piezo-electric actuator, and the reflectance signal is measured with a lockin-amplifier. When the conditions for SPR are fulfilled, the differential signal is zero. The shift of the resonance conditions can be measured as an increase of the differential signal, or using feedback on the angle of incidence. The sensor will be demonstrated by monitoring an adsorption and an immunoreaction at the sensor surface with sub-mdeg. resolution. The modulated incident beam can also be scanned past a number of sensing areas, making multichannel measurements possible, as will be demonstrated.",

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T1 - Differential SPR immunosensing

AU - Berger, Charles E.H.

AU - Berger, C.E.H.

AU - Greve, Jan

PY - 2000

Y1 - 2000

N2 - In this work we describe a surface plasmon resonance (SPR) sensor with a differential detection of the SPR angle, and demonstrate it. The angle of incidence is modulated by a simple piezo-electric actuator, and the reflectance signal is measured with a lockin-amplifier. When the conditions for SPR are fulfilled, the differential signal is zero. The shift of the resonance conditions can be measured as an increase of the differential signal, or using feedback on the angle of incidence. The sensor will be demonstrated by monitoring an adsorption and an immunoreaction at the sensor surface with sub-mdeg. resolution. The modulated incident beam can also be scanned past a number of sensing areas, making multichannel measurements possible, as will be demonstrated.

AB - In this work we describe a surface plasmon resonance (SPR) sensor with a differential detection of the SPR angle, and demonstrate it. The angle of incidence is modulated by a simple piezo-electric actuator, and the reflectance signal is measured with a lockin-amplifier. When the conditions for SPR are fulfilled, the differential signal is zero. The shift of the resonance conditions can be measured as an increase of the differential signal, or using feedback on the angle of incidence. The sensor will be demonstrated by monitoring an adsorption and an immunoreaction at the sensor surface with sub-mdeg. resolution. The modulated incident beam can also be scanned past a number of sensing areas, making multichannel measurements possible, as will be demonstrated.

KW - Surface plasmon resonance

KW - METIS-128419

KW - Immunosensor

KW - IR-74263

U2 - 10.1016/S0925-4005(00)00307-5

DO - 10.1016/S0925-4005(00)00307-5

M3 - Article

SN - 0925-4005

VL - 63

SP - 103

EP - 108

JO - Sensors and Actuators B: Chemical

JF - Sensors and Actuators B: Chemical

IS - 1-2

ER -