Diffusion from steady-state profile (DSSP) for low cost, low concentration measurement of diffusion

Joshua Taylor Loessberg-Zahl; Mark Gillrie; Roger D Kamm; Albert  van den Berg; A. van der Meer; Jan C.T. Eijkel

Diffusion from steady-state profile (DSSP) for low cost, low concentration measurement of diffusion

Joshua Taylor Loessberg-Zahl, Mark Gillrie, Roger D Kamm, Albert van den Berg, A. van der Meer, Jan C.T. Eijkel

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

3 Downloads (Pure)

Abstract

Here we present Diffusion from Steady-State Profile (DSSP), a simple, low-cost technique to measure the diffusivity of labeled proteins in hydrogels that are typically used for 3D cell culture. This is a steady-state technique which allows the use of long-exposure imaging, thereby enabling operation with low protein concentrations without the need for relatively expensive imaging equipment or immunoassays.

Original language	English
Title of host publication	23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences
Subtitle of host publication	uTAS 2019
Place of Publication	Basel, Switzerland
Publisher	The Chemical and Biological Microsystems Society
Pages	1530-1531
ISBN (Electronic)	978-1-7334190-0-0
Publication status	Published - 31 Oct 2019
Event	23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2019 - Congress Center Basel, Basel, Switzerland Duration: 27 Oct 2019 → 31 Oct 2019 Conference number: 23 https://cbmsociety.org/microtas/microtas2019/

Conference

Conference	23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2019
Abbreviated title	MicroTAS 2019
Country/Territory	Switzerland
City	Basel
Period	27/10/19 → 31/10/19
Internet address	https://cbmsociety.org/microtas/microtas2019/

Cite this

Loessberg-Zahl, J. T., Gillrie, M., Kamm, R. D., van den Berg, A., van der Meer, A., & Eijkel, J. C. T. (2019). Diffusion from steady-state profile (DSSP) for low cost, low concentration measurement of diffusion. In 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences: uTAS 2019 (pp. 1530-1531). The Chemical and Biological Microsystems Society.

@inproceedings{02fa535f4b1e4c6699218447d48ee1f9,

title = "Diffusion from steady-state profile (DSSP) for low cost, low concentration measurement of diffusion",

abstract = "Here we present Diffusion from Steady-State Profile (DSSP), a simple, low-cost technique to measure the diffusivity of labeled proteins in hydrogels that are typically used for 3D cell culture. This is a steady-state technique which allows the use of long-exposure imaging, thereby enabling operation with low protein concentrations without the need for relatively expensive imaging equipment or immunoassays. ",

author = "Loessberg-Zahl, {Joshua Taylor} and Mark Gillrie and Kamm, {Roger D} and {van den Berg}, Albert and {van der Meer}, A. and Eijkel, {Jan C.T.}",

year = "2019",

month = oct,

day = "31",

language = "English",

pages = "1530--1531",

booktitle = "23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences",

publisher = "The Chemical and Biological Microsystems Society",

note = "23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2019, MicroTAS 2019 ; Conference date: 27-10-2019 Through 31-10-2019",

url = "https://cbmsociety.org/microtas/microtas2019/",

}

Loessberg-Zahl, JT, Gillrie, M, Kamm, RD, van den Berg, A , van der Meer, A & Eijkel, JCT 2019, Diffusion from steady-state profile (DSSP) for low cost, low concentration measurement of diffusion. in 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences: uTAS 2019. The Chemical and Biological Microsystems Society, Basel, Switzerland, pp. 1530-1531, 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2019, Basel, Switzerland, 27/10/19.

Diffusion from steady-state profile (DSSP) for low cost, low concentration measurement of diffusion. / Loessberg-Zahl, Joshua Taylor; Gillrie, Mark; Kamm, Roger D et al.
23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences: uTAS 2019. Basel, Switzerland: The Chemical and Biological Microsystems Society, 2019. p. 1530-1531.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

TY - GEN

T1 - Diffusion from steady-state profile (DSSP) for low cost, low concentration measurement of diffusion

AU - Loessberg-Zahl, Joshua Taylor

AU - Gillrie, Mark

AU - Kamm, Roger D

AU - van den Berg, Albert

AU - van der Meer, A.

AU - Eijkel, Jan C.T.

N1 - Conference code: 23

PY - 2019/10/31

Y1 - 2019/10/31

N2 - Here we present Diffusion from Steady-State Profile (DSSP), a simple, low-cost technique to measure the diffusivity of labeled proteins in hydrogels that are typically used for 3D cell culture. This is a steady-state technique which allows the use of long-exposure imaging, thereby enabling operation with low protein concentrations without the need for relatively expensive imaging equipment or immunoassays.

AB - Here we present Diffusion from Steady-State Profile (DSSP), a simple, low-cost technique to measure the diffusivity of labeled proteins in hydrogels that are typically used for 3D cell culture. This is a steady-state technique which allows the use of long-exposure imaging, thereby enabling operation with low protein concentrations without the need for relatively expensive imaging equipment or immunoassays.

M3 - Conference contribution

SP - 1530

EP - 1531

BT - 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences

PB - The Chemical and Biological Microsystems Society

CY - Basel, Switzerland

T2 - 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2019

Y2 - 27 October 2019 through 31 October 2019

ER -

Loessberg-Zahl JT, Gillrie M, Kamm RD, van den Berg A , van der Meer A , Eijkel JCT. Diffusion from steady-state profile (DSSP) for low cost, low concentration measurement of diffusion. In 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences: uTAS 2019. Basel, Switzerland: The Chemical and Biological Microsystems Society. 2019. p. 1530-1531

Diffusion from steady-state profile (DSSP) for low cost, low concentration measurement of diffusion

Abstract

Conference

Fingerprint

Cite this