Diffusion from steady-state profile (DSSP) for low cost, low concentration measurement of diffusion

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Abstract

Here we present Diffusion from Steady-State Profile (DSSP), a simple, low-cost technique to measure the diffusivity of labeled proteins in hydrogels that are typically used for 3D cell culture. This is a steady-state technique which allows the use of long-exposure imaging, thereby enabling operation with low protein concentrations without the need for relatively expensive imaging equipment or immunoassays.
Original languageEnglish
Title of host publication23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences
Subtitle of host publicationuTAS 2019
Place of PublicationBasel, Switzerland
PublisherChemical and Biological Microssytems Society
Pages1530-1531
ISBN (Electronic)978-1-7334190-0-0
Publication statusPublished - 31 Oct 2019
Event23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2019 - Congress Center Basel, Basel, Switzerland
Duration: 27 Oct 201931 Oct 2019
Conference number: 23
https://cbmsociety.org/microtas/microtas2019/

Conference

Conference23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2019
Abbreviated titleMicroTAS 2019
CountrySwitzerland
CityBasel
Period27/10/1931/10/19
Internet address

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  • Cite this

    Loessberg-Zahl, J. T., Gillrie, M., Kamm, R. D., van den Berg, A., van der Meer, A., & Eijkel, J. C. T. (2019). Diffusion from steady-state profile (DSSP) for low cost, low concentration measurement of diffusion. In 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences: uTAS 2019 (pp. 1530-1531). Basel, Switzerland: Chemical and Biological Microssytems Society.