Direct measurement of lithium in whole blood using microchip capillary electrophoresis with integrated conductivity detection

E.X. Vrouwe, Regina Lüttge, Albert van den Berg

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The direct measurement of lithium in whole blood is described. Using microchip capillary electrophoresis (CE) with defined sample loading and applying the principles of column coupling, alkali metals were determined in a drop of whole blood. Blood collected from a finger stick was mixed with anticoagulant and transferred onto the chip without extraction or removal of components. The electrokinetic transport of red blood cells inside the channels was studied to find sample loading conditions suitable for the analysis of lithium without injecting cells into the separation channel. Both bare glass chips and chips coated with polyacrylamide were used showing the behavior of the cells under different electroosmotic flow conditions. In serum a detection limit for lithium of 0.4 mmol/L was reached. Proteins quickly contaminated untreated chip surfaces but devices with coating gave reproducible electropherograms. In addition, potassium and sodium were also detected in the same separation run. To our knowledge, this is the first device to directly measure ions in whole blood with the use of capillary zone electrophoresis on a microchip.
Original languageUndefined
Pages (from-to)1660-1667
Number of pages7
Issue number10-11
Publication statusPublished - 2004


  • METIS-218846
  • IR-47874
  • Conductivity detection
  • Miniaturization
  • Micro-fluidics
  • Microchip Capillary Electrophoresis
  • Lithium monitoring

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