Directed assembly of functional light harvesting antenna complexes onto chemically patterned surfaces

M. Escalante Marun, P.A. Maury, C.M. Bruinink, Kees van der Werf, John D. Olsen, A. Timney, Jurriaan Huskens, C. Neil Hunter, Vinod Subramaniam, Cornelis Otto

Research output: Contribution to journalArticleAcademicpeer-review

19 Citations (Scopus)

Abstract

We report the directed assembly of the photosynthetic membrane proteins LH1 and LH2 isolated from the purple bacterium Rhodobacter sphaeroides onto chemically patterned substrates. Nanoimprint lithography was used to pattern discrete regions of amino- and fluoro-terminated or poly(ethylene glycol) self-assembled monolayers onto a glass substrate. Densely packed layers of assembled protein complexes were observed with atomic force microscopy. The protein complexes attached selectively to the amino-terminated regions by electrostatic interactions. Spectral images generated with a hybrid scanning probe and fluorescence microscope confirmed that the patterned proteins retained their native optical signatures.
Original languageUndefined
Pages (from-to)025101-
JournalNanotechnology
Volume19
Issue number2
DOIs
Publication statusPublished - 2008

Keywords

  • METIS-248317
  • IR-61050

Cite this

Escalante Marun, M., Maury, P. A., Bruinink, C. M., van der Werf, K., Olsen, J. D., Timney, A., ... Otto, C. (2008). Directed assembly of functional light harvesting antenna complexes onto chemically patterned surfaces. Nanotechnology, 19(2), 025101-. https://doi.org/10.1088/0957-4484/19/02/025101