Directly immobilized DNA sensor for label-free detection of herpes virus

P. D. Tam, M. A. Tuan, Tom Aarnink, N. D. Chien

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

2 Citations (Scopus)

Abstract

This paper reports the direct immobilization of deoxyribonucleic acid (DNA) sequences of Herpes simplex virus (5'-AT CAC CGA CCC GGA GAG GGA C-3') on the surface of DNA sensor by using the cyclic voltammetric method with the presence of pyrrole. The potential was scanned from -0.7 volt to + 0.6 volt, the scanning rate was at 100 mV/s....This kind of DNA sensor was developed to detect Herpes virus DNA in samples. The FTIR was applied to verify specific binding of DNA sequence and conducting polymer, the morphology of conducting polymer doped with DNA strands was investigated by using a field emission scanning electron microscope (FESEM). The results showed that output signal given by coimmobilized DNA/PPy membrane sensor was better than that given by APTS immobilized membrane sensors. The sensor can detect as low as 2 nM of DNA target in real samples.

Original languageEnglish
Title of host publication5th Int. Conference on Information Technology and Applications in Biomedicine, ITAB 2008 in conjunction with 2nd Int. Symposium and Summer School on Biomedical and Health Engineering, IS3BHE 2008
Pages214-218
Number of pages5
DOIs
Publication statusPublished - 18 Jul 2008
Event5th International Conference on Information Technology and Applications in Biomedicine, ITAB 2008 - Shenzhen, China
Duration: 30 May 200831 May 2008
Conference number: 5

Publication series

Name5th Int. Conference on Information Technology and Applications in Biomedicine, ITAB 2008 in conjunction with 2nd Int. Symposium and Summer School on Biomedical and Health Engineering, IS3BHE 2008

Conference

Conference5th International Conference on Information Technology and Applications in Biomedicine, ITAB 2008
Abbreviated titleITAB 2008
Country/TerritoryChina
CityShenzhen
Period30/05/0831/05/08
OtherIn conjunction with 2nd International Symposium and Summer School on Biomedical and Health Engineering, IS3BHE 2008

Keywords

  • 2024 OA procedure

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