Discrimination of human cytotoxic lymphocytes from regulatory and B-lymphocytes by orthogonal light scattering

Leonardus Wendelinus Mathias Marie Terstappen; B.G. de Grooth; C.H.H. ten Napel; W. van Berkel; Jan Greve

doi:10.1016/0022-1759(86)90408-4

Discrimination of human cytotoxic lymphocytes from regulatory and B-lymphocytes by orthogonal light scattering

Leonardus Wendelinus Mathias Marie Terstappen, B.G. de Grooth, C.H.H. ten Napel, W. van Berkel, Jan Greve

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Abstract

Light scattering properties of human lymphocyte subpopulations selected by immunofluorescence were studied with a flow cytometer. Regulatory and B-lymphocytes showed a low orthogonal light scatter signal, whereas cytotoxic lymphocytes identified with leu-7, leu-11 and leu-15 revealed a large orthogonal light scatter signal. Two populations in light scatter histograms could be observed with monoclonal antibodies directed against determinants present on both regulatory and cytotoxic lymphocytes. By analysis of the lymphocytes of 16 individuals we found a linear relation between the number of cells with a large orthogonal light scattering and the number of cytotoxic lymphocytes identified with leu-7, leu-11 and leu-15. These observations demonstrate physical differences between cytotoxic lymphocytes and regulatory and B lymphocytes. Moreover, the results suggest a method to estimate the amount of cytotoxic lymphocytes without using monoclonal antibodies.

Original language	Undefined
Pages (from-to)	211-216
Journal	Journal of immunological methods
Volume	95
Issue number	2
DOIs	https://doi.org/10.1016/0022-1759(86)90408-4
Publication status	Published - 1986

Keywords

Orthogonal light scattering
IR-69748
Cytotoxic lymphocyte
Flow cytometry

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10.1016/0022-1759(86)90408-4

Terstappen86discrimination

Cite this

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title = "Discrimination of human cytotoxic lymphocytes from regulatory and B-lymphocytes by orthogonal light scattering",

abstract = "Light scattering properties of human lymphocyte subpopulations selected by immunofluorescence were studied with a flow cytometer. Regulatory and B-lymphocytes showed a low orthogonal light scatter signal, whereas cytotoxic lymphocytes identified with leu-7, leu-11 and leu-15 revealed a large orthogonal light scatter signal. Two populations in light scatter histograms could be observed with monoclonal antibodies directed against determinants present on both regulatory and cytotoxic lymphocytes. By analysis of the lymphocytes of 16 individuals we found a linear relation between the number of cells with a large orthogonal light scattering and the number of cytotoxic lymphocytes identified with leu-7, leu-11 and leu-15. These observations demonstrate physical differences between cytotoxic lymphocytes and regulatory and B lymphocytes. Moreover, the results suggest a method to estimate the amount of cytotoxic lymphocytes without using monoclonal antibodies.",

keywords = "Orthogonal light scattering, IR-69748, Cytotoxic lymphocyte, Flow cytometry",

author = "Terstappen, {Leonardus Wendelinus Mathias Marie} and {de Grooth}, B.G. and {ten Napel}, C.H.H. and {van Berkel}, W. and Jan Greve",

year = "1986",

doi = "10.1016/0022-1759(86)90408-4",

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pages = "211--216",

journal = "Journal of immunological methods",

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TY - JOUR

T1 - Discrimination of human cytotoxic lymphocytes from regulatory and B-lymphocytes by orthogonal light scattering

AU - Terstappen, Leonardus Wendelinus Mathias Marie

AU - de Grooth, B.G.

AU - ten Napel, C.H.H.

AU - van Berkel, W.

AU - Greve, Jan

PY - 1986

Y1 - 1986

N2 - Light scattering properties of human lymphocyte subpopulations selected by immunofluorescence were studied with a flow cytometer. Regulatory and B-lymphocytes showed a low orthogonal light scatter signal, whereas cytotoxic lymphocytes identified with leu-7, leu-11 and leu-15 revealed a large orthogonal light scatter signal. Two populations in light scatter histograms could be observed with monoclonal antibodies directed against determinants present on both regulatory and cytotoxic lymphocytes. By analysis of the lymphocytes of 16 individuals we found a linear relation between the number of cells with a large orthogonal light scattering and the number of cytotoxic lymphocytes identified with leu-7, leu-11 and leu-15. These observations demonstrate physical differences between cytotoxic lymphocytes and regulatory and B lymphocytes. Moreover, the results suggest a method to estimate the amount of cytotoxic lymphocytes without using monoclonal antibodies.

AB - Light scattering properties of human lymphocyte subpopulations selected by immunofluorescence were studied with a flow cytometer. Regulatory and B-lymphocytes showed a low orthogonal light scatter signal, whereas cytotoxic lymphocytes identified with leu-7, leu-11 and leu-15 revealed a large orthogonal light scatter signal. Two populations in light scatter histograms could be observed with monoclonal antibodies directed against determinants present on both regulatory and cytotoxic lymphocytes. By analysis of the lymphocytes of 16 individuals we found a linear relation between the number of cells with a large orthogonal light scattering and the number of cytotoxic lymphocytes identified with leu-7, leu-11 and leu-15. These observations demonstrate physical differences between cytotoxic lymphocytes and regulatory and B lymphocytes. Moreover, the results suggest a method to estimate the amount of cytotoxic lymphocytes without using monoclonal antibodies.

KW - Orthogonal light scattering

KW - IR-69748

KW - Cytotoxic lymphocyte

KW - Flow cytometry

U2 - 10.1016/0022-1759(86)90408-4

DO - 10.1016/0022-1759(86)90408-4

M3 - Article

VL - 95

SP - 211

EP - 216

JO - Journal of immunological methods

JF - Journal of immunological methods

SN - 0022-1759

IS - 2

ER -