Dissociated neurons from an extended rat subthalamic area - spontaneous activity and acetylcholine addition

Tjitske Heida; Enrico Marani

doi:10.1007/978-3-642-13039-7_45

Dissociated neurons from an extended rat subthalamic area - spontaneous activity and acetylcholine addition

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

Abstract

The tegmental nucleus pedunculopontinus proves to connect by acetylcholine neurotransmitters, among others, the subthalamic nucleus. Dissociated cultures of the subthalamic nucleus on micro-electrode arrays were studied by addition of acetylcholine and by stimulation. Addition of acetylcholine had no effect on bursting activity, reduced spike activity strongly for a short period, after which activity increased again, but overall activity was reduced over time with 25%. After termination of acetylcholine addition the activity returned to normal.

Original language	Undefined
Title of host publication	XII Mediterranean Conference on Medical and Biological Engineering and Computing
Place of Publication	Heidelberg
Publisher	Springer
Pages	180-183
Number of pages	4
ISBN (Print)	978-3-642-13038-0
DOIs	https://doi.org/10.1007/978-3-642-13039-7_45
Publication status	Published - 27 May 2010
Event	XII Mediterranean Conference on Medical and Biological Engineering and Computing 2010 - Chalkidiki, Greece, Chalcidice, Greece Duration: 27 May 2010 → 30 May 2010 Conference number: 12 http://www.medicon2010.org/

Publication series

Name	IFMBE Proceedings
Publisher	Springer Verlag
Number	29
Volume	29
ISSN (Print)	1680-0737
ISSN (Electronic)	1433-9277

Conference

Conference	XII Mediterranean Conference on Medical and Biological Engineering and Computing 2010
Abbreviated title	MEDICON 2010
Country/Territory	Greece
City	Chalcidice
Period	27/05/10 → 30/05/10
Other	27-30 May 2010
Internet address	http://www.medicon2010.org/

Keywords

METIS-270971
IR-72538
acetylcholine
EWI-18279
subthalamic area
Parkinson’s disease
Dissociated neurons

Access to Document

10.1007/978-3-642-13039-7_45

http://eprints.eemcs.utwente.nl/secure2/18279/01/Heida_Medicon_2010_180-183.pdf

Cite this

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title = "Dissociated neurons from an extended rat subthalamic area - spontaneous activity and acetylcholine addition",

abstract = "The tegmental nucleus pedunculopontinus proves to connect by acetylcholine neurotransmitters, among others, the subthalamic nucleus. Dissociated cultures of the subthalamic nucleus on micro-electrode arrays were studied by addition of acetylcholine and by stimulation. Addition of acetylcholine had no effect on bursting activity, reduced spike activity strongly for a short period, after which activity increased again, but overall activity was reduced over time with 25%. After termination of acetylcholine addition the activity returned to normal.",

keywords = "METIS-270971, IR-72538, acetylcholine, EWI-18279, subthalamic area, Parkinson{\textquoteright}s disease, Dissociated neurons",

author = "Tjitske Heida and Enrico Marani",

note = "10.1007/978-3-642-13039-7_45 ; null ; Conference date: 27-05-2010 Through 30-05-2010",

year = "2010",

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language = "Undefined",

isbn = "978-3-642-13038-0",

series = "IFMBE Proceedings",

publisher = "Springer",

number = "29",

pages = "180--183",

booktitle = "XII Mediterranean Conference on Medical and Biological Engineering and Computing",

address = "Netherlands",

url = "http://www.medicon2010.org/",

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Heida, T & Marani, E 2010, Dissociated neurons from an extended rat subthalamic area - spontaneous activity and acetylcholine addition. in XII Mediterranean Conference on Medical and Biological Engineering and Computing. IFMBE Proceedings, no. 29, vol. 29, Springer, Heidelberg, pp. 180-183, XII Mediterranean Conference on Medical and Biological Engineering and Computing 2010, Chalcidice, Greece, 27/05/10. https://doi.org/10.1007/978-3-642-13039-7_45

Dissociated neurons from an extended rat subthalamic area - spontaneous activity and acetylcholine addition. / Heida, Tjitske ; Marani, Enrico.

XII Mediterranean Conference on Medical and Biological Engineering and Computing. Heidelberg : Springer, 2010. p. 180-183 (IFMBE Proceedings; Vol. 29, No. 29).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

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AB - The tegmental nucleus pedunculopontinus proves to connect by acetylcholine neurotransmitters, among others, the subthalamic nucleus. Dissociated cultures of the subthalamic nucleus on micro-electrode arrays were studied by addition of acetylcholine and by stimulation. Addition of acetylcholine had no effect on bursting activity, reduced spike activity strongly for a short period, after which activity increased again, but overall activity was reduced over time with 25%. After termination of acetylcholine addition the activity returned to normal.

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KW - acetylcholine

KW - EWI-18279

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KW - Parkinson’s disease

KW - Dissociated neurons

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