The movement of XbaI digested -DNA in 20 nanomometer and .. -DNA in 12 nanometer high slits was investigated. We found that DNA moved intermittently and following preferential pathways, indicating an important influence of surface roughness. From these mobilities and averaged mobilities between the intermittent sticking events. The friction coefficient per unit length was calculated from the latter mobilities. A three order of magnitude increase was found for the 12 nm slits compared to the theoretical value. The mobility furthermore differs less than one order of magnitude between 20 nm and 12 nm slits, and the influence of varying the ionic strength of the buffer was not significant. This work is the first time DNA movement in such shallow constrictions is investigated.
|Name||Micro Total Analysis Systems|
|Publisher||Chemical and Biological Microsystems Society|
|Conference||11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2007|
|Period||7/10/07 → 11/10/07|
- intermittent movement