DNA movement in sub-20 nm nanoslits

G.B. Beugelaar, Juliane Teapal, Jan William van Nieuwkasteele, Daniël Wijnperle, Jonas O. Tegenfeldt, Jan C.T. Eijkel, Albert van den Berg

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

2 Citations (Scopus)

Abstract

The movement of XbaI digested -DNA in 20 nanomometer and .. -DNA in 12 nanometer high slits was investigated. We found that DNA moved intermittently and following preferential pathways, indicating an important influence of surface roughness. From these mobilities and averaged mobilities between the intermittent sticking events. The friction coefficient per unit length was calculated from the latter mobilities. A three order of magnitude increase was found for the 12 nm slits compared to the theoretical value. The mobility furthermore differs less than one order of magnitude between 20 nm and 12 nm slits, and the influence of varying the ionic strength of the buffer was not significant. This work is the first time DNA movement in such shallow constrictions is investigated.
Original languageUndefined
Title of host publicationEleventh International Conference on Miniaturized Systems for Chemistry and Life Sciences - The proceedings of microTAS 2007 Conference
EditorsJ.L. Viovy, P Tabeling, S. Descroix, L. Malaquin
Place of PublicationSan Diego
PublisherThe Chemical and Biological Microsystems Society
Pages1201-1203
Number of pages3
ISBN (Print)978-0-9798064-0-7
Publication statusPublished - 7 Oct 2007
Event11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2007 - Paris, France
Duration: 7 Oct 200711 Oct 2007
Conference number: 11

Publication series

NameMicro Total Analysis Systems
PublisherChemical and Biological Microsystems Society
Number7
Volume2
ISSN (Print)1556-5890

Conference

Conference11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2007
Abbreviated titleMicroTAS
CountryFrance
CityParis
Period7/10/0711/10/07

Keywords

  • intermittent movement
  • EWI-11696
  • METIS-245922
  • DNA
  • Nanoslits
  • IR-62101

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