DNA-Origami Enabled Distance-Dependent Sensing

Jeanne E. Van Dongen; Jan C.T. Eijkel; Loes I. Segerink

doi:10.1109/SENSORS52175.2022.9967092

DNA-Origami Enabled Distance-Dependent Sensing

Jeanne E. Van Dongen, Jan C.T. Eijkel, Loes I. Segerink

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

Abstract

Localized surface plasmon resonance is a popular optical detection technique due to its ease of use. However, the main issues of this technique are its selectivity and sensitivity towards (small) single molecules. We address these challenges by introducing a distance-dependent plasmonic sensing method based on a gold nanoparticle (AuNP) tethered by a single DNA hairpin to a gold film (Au-film). The plasmonic properties of a AuNP enable us to distinguish the z-height of the particle. In the absence of a target sequence, the hairpin keeps the AuNP close to the Au-film, while hybridization with a target sequence results in an extended state of the DNA tether. We experimentally confirmed the assembly of this sensor and by simulations, predicted the plasmonic signal of the two states. Finally, we showed experimental results as proof of concept of our sensing method.

Original language	English
Title of host publication	2022 IEEE Sensors
Place of Publication	Dallas
Publisher	IEEE
ISBN (Electronic)	978-1-6654-8464-0
ISBN (Print)	78-1-6654-8465-7
DOIs	https://doi.org/10.1109/SENSORS52175.2022.9967092
Publication status	Published - 8 Dec 2022
Event	IEEE SENSORS 2022 - US, Dalas, United States Duration: 30 Oct 2022 → 2 Nov 2022 https://2022.ieee-sensorsconference.org/

Conference

Conference	IEEE SENSORS 2022
Country/Territory	United States
City	Dalas
Period	30/10/22 → 2/11/22
Internet address	https://2022.ieee-sensorsconference.org/

Keywords

22/4 OA procedure

Access to Document

10.1109/SENSORS52175.2022.9967092

Cite this

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title = "DNA-Origami Enabled Distance-Dependent Sensing",

abstract = "Localized surface plasmon resonance is a popular optical detection technique due to its ease of use. However, the main issues of this technique are its selectivity and sensitivity towards (small) single molecules. We address these challenges by introducing a distance-dependent plasmonic sensing method based on a gold nanoparticle (AuNP) tethered by a single DNA hairpin to a gold film (Au-film). The plasmonic properties of a AuNP enable us to distinguish the z-height of the particle. In the absence of a target sequence, the hairpin keeps the AuNP close to the Au-film, while hybridization with a target sequence results in an extended state of the DNA tether. We experimentally confirmed the assembly of this sensor and by simulations, predicted the plasmonic signal of the two states. Finally, we showed experimental results as proof of concept of our sensing method.",

keywords = "22/4 OA procedure",

author = "Dongen, {Jeanne E. Van} and Eijkel, {Jan C.T.} and Segerink, {Loes I.}",

year = "2022",

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language = "English",

isbn = "78-1-6654-8465-7",

booktitle = "2022 IEEE Sensors",

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url = "https://2022.ieee-sensorsconference.org/",

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TY - GEN

T1 - DNA-Origami Enabled Distance-Dependent Sensing

AU - Dongen, Jeanne E. Van

AU - Eijkel, Jan C.T.

AU - Segerink, Loes I.

PY - 2022/12/8

Y1 - 2022/12/8

N2 - Localized surface plasmon resonance is a popular optical detection technique due to its ease of use. However, the main issues of this technique are its selectivity and sensitivity towards (small) single molecules. We address these challenges by introducing a distance-dependent plasmonic sensing method based on a gold nanoparticle (AuNP) tethered by a single DNA hairpin to a gold film (Au-film). The plasmonic properties of a AuNP enable us to distinguish the z-height of the particle. In the absence of a target sequence, the hairpin keeps the AuNP close to the Au-film, while hybridization with a target sequence results in an extended state of the DNA tether. We experimentally confirmed the assembly of this sensor and by simulations, predicted the plasmonic signal of the two states. Finally, we showed experimental results as proof of concept of our sensing method.

AB - Localized surface plasmon resonance is a popular optical detection technique due to its ease of use. However, the main issues of this technique are its selectivity and sensitivity towards (small) single molecules. We address these challenges by introducing a distance-dependent plasmonic sensing method based on a gold nanoparticle (AuNP) tethered by a single DNA hairpin to a gold film (Au-film). The plasmonic properties of a AuNP enable us to distinguish the z-height of the particle. In the absence of a target sequence, the hairpin keeps the AuNP close to the Au-film, while hybridization with a target sequence results in an extended state of the DNA tether. We experimentally confirmed the assembly of this sensor and by simulations, predicted the plasmonic signal of the two states. Finally, we showed experimental results as proof of concept of our sensing method.

KW - 22/4 OA procedure

UR - https://doi.org/10.1109/SENSORS52175.2022.9967092

U2 - 10.1109/SENSORS52175.2022.9967092

DO - 10.1109/SENSORS52175.2022.9967092

M3 - Conference contribution

SN - 78-1-6654-8465-7

BT - 2022 IEEE Sensors

PB - IEEE

CY - Dallas

T2 - IEEE SENSORS 2022

Y2 - 30 October 2022 through 2 November 2022

ER -

DNA-Origami Enabled Distance-Dependent Sensing

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Keywords

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