DNA Separation and Fluorescence Monitoring by Integrated Waveguides in an Optofluidic Chip

C. Dongre, J. van Weerd, G.A.J. Besselink, R. Martinez-Vazquez, R. Osellame, R. Ramponi, G. Cerullo, R. van Weeghel, H.H. van den Vlekkert, Hugo Hoekstra, Markus Pollnau

    Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

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    Abstract

    We report on the monolithic integration of optical waveguides and microfluidics in a fused-silica lab-on-a-chip. Labeled biomolecules such as double-stranded DNA are flown and separated in the microfluidic channel by capillary electrophoresis and their fluorescent labels are excited by a continuous-wave laser beam through femtosecondlaser-written integrated waveguides. In this context, desirable features such as high spatial resolution (~12 μm), and a low limit of detection (~ 6 nano-molar) have been experimentally demonstrated. The proof of concept is being extended to real-world diagnostic samples for on-chip diagnosis of genetic diseases, e.g. breast cancer.
    Original languageEnglish
    Title of host publicationProceedings of the 2009 Annual Symposium of the IEEE Photonics Benelux Chapter
    EditorsStefano Beri, Philippe Tassin, Gordon Craggs, Xaveer Leijtens, Jan Dankaert
    PublisherVUBPress Brussels University Press
    Pages49-52
    Number of pages4
    ISBN (Print)978-90-5487-650-2
    Publication statusPublished - 5 Nov 2009
    Event14th Annual Symposium of the IEEE Photonics Benelux Chapter 2009 - Brussels, Belgium
    Duration: 5 Nov 20096 Nov 2009
    Conference number: 14

    Conference

    Conference14th Annual Symposium of the IEEE Photonics Benelux Chapter 2009
    CountryBelgium
    CityBrussels
    Period5/11/096/11/09

    Keywords

    • DNA
    • IOMS-SNS: SENSORS
    • Sensor
    • EC Grant Agreement nr.: FP6/034562
    • Optofluidic

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