DNA Separation and Fluorescence Monitoring by Integrated Waveguides in an Optofluidic Chip

C. Dongre; J. van Weerd; G.A.J. Besselink; R. Martinez-Vazquez; R. Osellame; R. Ramponi; G. Cerullo; R. van Weeghel; H.H. van den Vlekkert; Hugo Hoekstra; Markus Pollnau

DNA Separation and Fluorescence Monitoring by Integrated Waveguides in an Optofluidic Chip

C. Dongre, J. van Weerd, G.A.J. Besselink, R. Martinez-Vazquez, R. Osellame, R. Ramponi, G. Cerullo, R. van Weeghel, H.H. van den Vlekkert, Hugo Hoekstra, Markus Pollnau

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

13 Downloads (Pure)

Abstract

We report on the monolithic integration of optical waveguides and microfluidics in a fused-silica lab-on-a-chip. Labeled biomolecules such as double-stranded DNA are flown and separated in the microfluidic channel by capillary electrophoresis and their fluorescent labels are excited by a continuous-wave laser beam through femtosecondlaser-written integrated waveguides. In this context, desirable features such as high spatial resolution (~12 μm), and a low limit of detection (~ 6 nano-molar) have been experimentally demonstrated. The proof of concept is being extended to real-world diagnostic samples for on-chip diagnosis of genetic diseases, e.g. breast cancer.

Original language	English
Title of host publication	Proceedings of the 2009 Annual Symposium of the IEEE Photonics Benelux Chapter
Editors	Stefano Beri, Philippe Tassin, Gordon Craggs, Xaveer Leijtens, Jan Dankaert
Publisher	VUBPress Brussels University Press
Pages	49-52
Number of pages	4
ISBN (Print)	978-90-5487-650-2
Publication status	Published - 5 Nov 2009
Event	14th Annual Symposium of the IEEE Photonics Benelux Chapter 2009 - Brussels, Belgium Duration: 5 Nov 2009 → 6 Nov 2009 Conference number: 14

Conference

Conference	14th Annual Symposium of the IEEE Photonics Benelux Chapter 2009
Country/Territory	Belgium
City	Brussels
Period	5/11/09 → 6/11/09

Keywords

DNA
IOMS-SNS: SENSORS
Sensor
EC Grant Agreement nr.: FP6/034562
Optofluidic

Access to Document

http://www.photonics-benelux.org

Cite this

Dongre, C., van Weerd, J., Besselink, G. A. J., Martinez-Vazquez, R., Osellame, R., Ramponi, R., Cerullo, G., van Weeghel, R., van den Vlekkert, H. H., Hoekstra, H., & Pollnau, M. (2009). DNA Separation and Fluorescence Monitoring by Integrated Waveguides in an Optofluidic Chip. In S. Beri, P. Tassin, G. Craggs, X. Leijtens, & J. Dankaert (Eds.), Proceedings of the 2009 Annual Symposium of the IEEE Photonics Benelux Chapter (pp. 49-52). VUBPress Brussels University Press. http://www.photonics-benelux.org

@inproceedings{8356c51605ae470a82888280249d99bb,

title = "DNA Separation and Fluorescence Monitoring by Integrated Waveguides in an Optofluidic Chip",

abstract = "We report on the monolithic integration of optical waveguides and microfluidics in a fused-silica lab-on-a-chip. Labeled biomolecules such as double-stranded DNA are flown and separated in the microfluidic channel by capillary electrophoresis and their fluorescent labels are excited by a continuous-wave laser beam through femtosecondlaser-written integrated waveguides. In this context, desirable features such as high spatial resolution (~12 μm), and a low limit of detection (~ 6 nano-molar) have been experimentally demonstrated. The proof of concept is being extended to real-world diagnostic samples for on-chip diagnosis of genetic diseases, e.g. breast cancer.",

keywords = "DNA, IOMS-SNS: SENSORS, Sensor, EC Grant Agreement nr.: FP6/034562, Optofluidic",

author = "C. Dongre and {van Weerd}, J. and G.A.J. Besselink and R. Martinez-Vazquez and R. Osellame and R. Ramponi and G. Cerullo and {van Weeghel}, R. and {van den Vlekkert}, H.H. and Hugo Hoekstra and Markus Pollnau",

year = "2009",

month = nov,

day = "5",

language = "English",

isbn = "978-90-5487-650-2",

pages = "49--52",

editor = "Stefano Beri and Philippe Tassin and Gordon Craggs and Xaveer Leijtens and Jan Dankaert",

booktitle = "Proceedings of the 2009 Annual Symposium of the IEEE Photonics Benelux Chapter",

publisher = "VUBPress Brussels University Press",

note = "14th Annual Symposium of the IEEE Photonics Benelux Chapter 2009 ; Conference date: 05-11-2009 Through 06-11-2009",

}

Dongre, C, van Weerd, J, Besselink, GAJ, Martinez-Vazquez, R, Osellame, R, Ramponi, R, Cerullo, G, van Weeghel, R, van den Vlekkert, HH, Hoekstra, H & Pollnau, M 2009, DNA Separation and Fluorescence Monitoring by Integrated Waveguides in an Optofluidic Chip. in S Beri, P Tassin, G Craggs, X Leijtens & J Dankaert (eds), Proceedings of the 2009 Annual Symposium of the IEEE Photonics Benelux Chapter. VUBPress Brussels University Press, pp. 49-52, 14th Annual Symposium of the IEEE Photonics Benelux Chapter 2009, Brussels, Belgium, 5/11/09. <http://www.photonics-benelux.org>

DNA Separation and Fluorescence Monitoring by Integrated Waveguides in an Optofluidic Chip. / Dongre, C.; van Weerd, J.; Besselink, G.A.J. et al.
Proceedings of the 2009 Annual Symposium of the IEEE Photonics Benelux Chapter. ed. / Stefano Beri; Philippe Tassin; Gordon Craggs; Xaveer Leijtens; Jan Dankaert. VUBPress Brussels University Press, 2009. p. 49-52.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

TY - GEN

T1 - DNA Separation and Fluorescence Monitoring by Integrated Waveguides in an Optofluidic Chip

AU - Dongre, C.

AU - van Weerd, J.

AU - Besselink, G.A.J.

AU - Martinez-Vazquez, R.

AU - Osellame, R.

AU - Ramponi, R.

AU - Cerullo, G.

AU - van Weeghel, R.

AU - van den Vlekkert, H.H.

AU - Hoekstra, Hugo

AU - Pollnau, Markus

N1 - Conference code: 14

PY - 2009/11/5

Y1 - 2009/11/5

N2 - We report on the monolithic integration of optical waveguides and microfluidics in a fused-silica lab-on-a-chip. Labeled biomolecules such as double-stranded DNA are flown and separated in the microfluidic channel by capillary electrophoresis and their fluorescent labels are excited by a continuous-wave laser beam through femtosecondlaser-written integrated waveguides. In this context, desirable features such as high spatial resolution (~12 μm), and a low limit of detection (~ 6 nano-molar) have been experimentally demonstrated. The proof of concept is being extended to real-world diagnostic samples for on-chip diagnosis of genetic diseases, e.g. breast cancer.

AB - We report on the monolithic integration of optical waveguides and microfluidics in a fused-silica lab-on-a-chip. Labeled biomolecules such as double-stranded DNA are flown and separated in the microfluidic channel by capillary electrophoresis and their fluorescent labels are excited by a continuous-wave laser beam through femtosecondlaser-written integrated waveguides. In this context, desirable features such as high spatial resolution (~12 μm), and a low limit of detection (~ 6 nano-molar) have been experimentally demonstrated. The proof of concept is being extended to real-world diagnostic samples for on-chip diagnosis of genetic diseases, e.g. breast cancer.

KW - DNA

KW - IOMS-SNS: SENSORS

KW - Sensor

KW - EC Grant Agreement nr.: FP6/034562

KW - Optofluidic

M3 - Conference contribution

SN - 978-90-5487-650-2

SP - 49

EP - 52

BT - Proceedings of the 2009 Annual Symposium of the IEEE Photonics Benelux Chapter

A2 - Beri, Stefano

A2 - Tassin, Philippe

A2 - Craggs, Gordon

A2 - Leijtens, Xaveer

A2 - Dankaert, Jan

PB - VUBPress Brussels University Press

T2 - 14th Annual Symposium of the IEEE Photonics Benelux Chapter 2009

Y2 - 5 November 2009 through 6 November 2009

ER -

Dongre C, van Weerd J, Besselink GAJ, Martinez-Vazquez R, Osellame R, Ramponi R et al. DNA Separation and Fluorescence Monitoring by Integrated Waveguides in an Optofluidic Chip. In Beri S, Tassin P, Craggs G, Leijtens X, Dankaert J, editors, Proceedings of the 2009 Annual Symposium of the IEEE Photonics Benelux Chapter. VUBPress Brussels University Press. 2009. p. 49-52

DNA Separation and Fluorescence Monitoring by Integrated Waveguides in an Optofluidic Chip

Abstract

Conference

Keywords

Access to Document

Fingerprint

Cite this