DNA transport in 20 nm nanoslits by AC and DC electrical fields

G. B. Salieb-Beugelaar, Ó Castillo-Fernandez, M. Arundell, J. Samitier, A. Van Den Berg, J. C T Eijkel

    Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

    Abstract

    We investigated the transport of λ-DNA in 20 nm nanoslits under the influence of applied AC and DC fields. At DC fields below 15 kV/m it was found that the addition of 1 kHz AC fields with maximum strengths between 10 and 200 kV/m significantly increased the mobility. At DC fields above 15 kV/m no influence of the AC fields was observed. The observed behavior indicates that steric effects rather than dielectrophoretic effects are responsible for the observed low DNA mobility in 20 nm nanoslits. The observed mobility behaviour can be explained if the DNA moves by biased reptation in the nanoslits.

    Original languageEnglish
    Title of host publicationMicro total analysis systems 2009
    Subtitle of host publicationproceedings of MicroTAS 2009: 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, November 1-5, 2009, Jeju, Korea
    EditorsTae Song Kim
    Place of PublicationSan Diego, CA
    PublisherChemical and Biological Microstystems Society
    Pages1518-1520
    Number of pages3
    ISBN (Print)9780979806421
    Publication statusPublished - 1 Jan 2009
    Event13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2009 - Jeju, Korea, Republic of
    Duration: 1 Nov 20095 Nov 2009
    Conference number: 13

    Conference

    Conference13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2009
    Abbreviated titleMicroTAS
    CountryKorea, Republic of
    CityJeju
    Period1/11/095/11/09

    Keywords

    • Dielectrophoresis
    • DNA separation
    • Nanofluidics
    • Nanoslits

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