DNA transport in 20 nm nanoslits by AC and DC electrical fields

G. B. Salieb-Beugelaar; Ó Castillo-Fernandez; M. Arundell; J. Samitier; A. Van Den Berg; J. C T Eijkel

DNA transport in 20 nm nanoslits by AC and DC electrical fields

G. B. Salieb-Beugelaar, Ó Castillo-Fernandez, M. Arundell, J. Samitier, A. Van Den Berg, J. C T Eijkel

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

Abstract

We investigated the transport of λ-DNA in 20 nm nanoslits under the influence of applied AC and DC fields. At DC fields below 15 kV/m it was found that the addition of 1 kHz AC fields with maximum strengths between 10 and 200 kV/m significantly increased the mobility. At DC fields above 15 kV/m no influence of the AC fields was observed. The observed behavior indicates that steric effects rather than dielectrophoretic effects are responsible for the observed low DNA mobility in 20 nm nanoslits. The observed mobility behaviour can be explained if the DNA moves by biased reptation in the nanoslits.

Original language	English
Title of host publication	Micro total analysis systems 2009
Subtitle of host publication	proceedings of MicroTAS 2009: 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, November 1-5, 2009, Jeju, Korea
Editors	Tae Song Kim
Place of Publication	San Diego, CA
Publisher	Chemical and Biological Microstystems Society
Pages	1518-1520
Number of pages	3
ISBN (Print)	9780979806421
Publication status	Published - 1 Jan 2009
Event	13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2009 - Jeju, Korea, Republic of Duration: 1 Nov 2009 → 5 Nov 2009 Conference number: 13

Conference

Conference	13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2009
Abbreviated title	MicroTAS
Country	Korea, Republic of
City	Jeju
Period	1/11/09 → 5/11/09

Keywords

Dielectrophoresis
DNA separation
Nanofluidics
Nanoslits

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Salieb-Beugelaar, G. B., Castillo-Fernandez, Ó., Arundell, M., Samitier, J., Van Den Berg, A., & Eijkel, J. C. T. (2009). DNA transport in 20 nm nanoslits by AC and DC electrical fields. In T. S. Kim (Ed.), Micro total analysis systems 2009: proceedings of MicroTAS 2009: 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, November 1-5, 2009, Jeju, Korea (pp. 1518-1520). Chemical and Biological Microstystems Society.

Salieb-Beugelaar, G. B. ; Castillo-Fernandez, Ó ; Arundell, M. ; Samitier, J. ; Van Den Berg, A. ; Eijkel, J. C T. / DNA transport in 20 nm nanoslits by AC and DC electrical fields. Micro total analysis systems 2009: proceedings of MicroTAS 2009: 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, November 1-5, 2009, Jeju, Korea. editor / Tae Song Kim. San Diego, CA : Chemical and Biological Microstystems Society, 2009. pp. 1518-1520

@inproceedings{cd0fc42347174a20b625dbda48696e5f,

title = "DNA transport in 20 nm nanoslits by AC and DC electrical fields",

abstract = "We investigated the transport of λ-DNA in 20 nm nanoslits under the influence of applied AC and DC fields. At DC fields below 15 kV/m it was found that the addition of 1 kHz AC fields with maximum strengths between 10 and 200 kV/m significantly increased the mobility. At DC fields above 15 kV/m no influence of the AC fields was observed. The observed behavior indicates that steric effects rather than dielectrophoretic effects are responsible for the observed low DNA mobility in 20 nm nanoslits. The observed mobility behaviour can be explained if the DNA moves by biased reptation in the nanoslits.",

keywords = "Dielectrophoresis, DNA separation, Nanofluidics, Nanoslits",

author = "Salieb-Beugelaar, {G. B.} and {\'O} Castillo-Fernandez and M. Arundell and J. Samitier and {Van Den Berg}, A. and Eijkel, {J. C T}",

year = "2009",

month = jan,

day = "1",

language = "English",

isbn = "9780979806421",

pages = "1518--1520",

editor = "Kim, {Tae Song}",

booktitle = "Micro total analysis systems 2009",

publisher = "Chemical and Biological Microstystems Society",

note = "13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2009, MicroTAS ; Conference date: 01-11-2009 Through 05-11-2009",

}

Salieb-Beugelaar, GB, Castillo-Fernandez, Ó, Arundell, M, Samitier, J, Van Den Berg, A & Eijkel, JCT 2009, DNA transport in 20 nm nanoslits by AC and DC electrical fields. in TS Kim (ed.), Micro total analysis systems 2009: proceedings of MicroTAS 2009: 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, November 1-5, 2009, Jeju, Korea. Chemical and Biological Microstystems Society, San Diego, CA, pp. 1518-1520, 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2009, Jeju, Korea, Republic of, 1/11/09.

DNA transport in 20 nm nanoslits by AC and DC electrical fields. / Salieb-Beugelaar, G. B.; Castillo-Fernandez, Ó; Arundell, M.; Samitier, J.; Van Den Berg, A.; Eijkel, J. C T.

Micro total analysis systems 2009: proceedings of MicroTAS 2009: 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, November 1-5, 2009, Jeju, Korea. ed. / Tae Song Kim. San Diego, CA : Chemical and Biological Microstystems Society, 2009. p. 1518-1520.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

TY - GEN

T1 - DNA transport in 20 nm nanoslits by AC and DC electrical fields

AU - Salieb-Beugelaar, G. B.

AU - Castillo-Fernandez, Ó

AU - Arundell, M.

AU - Samitier, J.

AU - Van Den Berg, A.

AU - Eijkel, J. C T

N1 - Conference code: 13

PY - 2009/1/1

Y1 - 2009/1/1

N2 - We investigated the transport of λ-DNA in 20 nm nanoslits under the influence of applied AC and DC fields. At DC fields below 15 kV/m it was found that the addition of 1 kHz AC fields with maximum strengths between 10 and 200 kV/m significantly increased the mobility. At DC fields above 15 kV/m no influence of the AC fields was observed. The observed behavior indicates that steric effects rather than dielectrophoretic effects are responsible for the observed low DNA mobility in 20 nm nanoslits. The observed mobility behaviour can be explained if the DNA moves by biased reptation in the nanoslits.

AB - We investigated the transport of λ-DNA in 20 nm nanoslits under the influence of applied AC and DC fields. At DC fields below 15 kV/m it was found that the addition of 1 kHz AC fields with maximum strengths between 10 and 200 kV/m significantly increased the mobility. At DC fields above 15 kV/m no influence of the AC fields was observed. The observed behavior indicates that steric effects rather than dielectrophoretic effects are responsible for the observed low DNA mobility in 20 nm nanoslits. The observed mobility behaviour can be explained if the DNA moves by biased reptation in the nanoslits.

KW - Dielectrophoresis

KW - DNA separation

KW - Nanofluidics

KW - Nanoslits

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M3 - Conference contribution

AN - SCOPUS:84901792461

SN - 9780979806421

SP - 1518

EP - 1520

BT - Micro total analysis systems 2009

A2 - Kim, Tae Song

PB - Chemical and Biological Microstystems Society

CY - San Diego, CA

T2 - 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2009

Y2 - 1 November 2009 through 5 November 2009

ER -

Salieb-Beugelaar GB, Castillo-Fernandez Ó, Arundell M, Samitier J, Van Den Berg A , Eijkel JCT. DNA transport in 20 nm nanoslits by AC and DC electrical fields. In Kim TS, editor, Micro total analysis systems 2009: proceedings of MicroTAS 2009: 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, November 1-5, 2009, Jeju, Korea. San Diego, CA: Chemical and Biological Microstystems Society. 2009. p. 1518-1520