Abstract
We investigated the transport of λ-DNA in 20 nm nanoslits under the influence of applied AC and DC fields. At DC fields below 15 kV/m it was found that the addition of 1 kHz AC fields with maximum strengths between 10 and 200 kV/m significantly increased the mobility. At DC fields above 15 kV/m no influence of the AC fields was observed. The observed behavior indicates that steric effects rather than dielectrophoretic effects are responsible for the observed low DNA mobility in 20 nm nanoslits. The observed mobility behaviour can be explained if the DNA moves by biased reptation in the nanoslits.
Original language | English |
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Title of host publication | Micro total analysis systems 2009 |
Subtitle of host publication | proceedings of MicroTAS 2009: 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, November 1-5, 2009, Jeju, Korea |
Editors | Tae Song Kim |
Place of Publication | San Diego, CA |
Publisher | The Chemical and Biological Microsystems Society |
Pages | 1518-1520 |
Number of pages | 3 |
ISBN (Print) | 9780979806421 |
Publication status | Published - 1 Jan 2009 |
Event | 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2009 - Jeju, Korea, Republic of Duration: 1 Nov 2009 → 5 Nov 2009 Conference number: 13 |
Conference
Conference | 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2009 |
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Abbreviated title | MicroTAS |
Country/Territory | Korea, Republic of |
City | Jeju |
Period | 1/11/09 → 5/11/09 |
Keywords
- Dielectrophoresis
- DNA separation
- Nanofluidics
- Nanoslits