DNA transport in 20nm nanoslits by AC and DC electrical fields

G.B. Beugelaar, O. Castillo-Fernandez, O. Castillo, M. Arundell, J. Samitier, Albert van den Berg, Jan C.T. Eijkel

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Abstract

We investigated the transport of λ-DNA in 20 nm nanoslits under the influence of applied AC and DC fields. At DC fields below 15 kV/m it was found that the addition of 1 kHz AC fields with maximum strengths between 10 and 200 kV/m significantly increased the mobility. At DC fields above 15 kV/m no influence of the AC fields was observed. The observed behavior indicates that steric effects rather than dielectrophoretic effects are responsible for the observed low DNA mobility in 20 nm nanoslits. The observed mobility behaviour can be explained if the DNA moves by biased reptation in the nanoslits
Original languageUndefined
Title of host publicationMicro Total Analysis Systems 2009
EditorsT.S. Kim, Y-S Lee, T-D Chung, N.L. Jeon, S.H. Lee, K-Y Suh, J. Choo, Y-K Kim
Place of PublicationSan Diego
PublisherChemical and Biological Society
Pages1518-1520
Number of pages3
ISBN (Print)978-0-9798064-2-1
Publication statusPublished - 1 Nov 2009
Event13th International Conference on Micro Total Analysis Systems, µTAS 2009 - Jeju Island, Korea, Republic of
Duration: 1 Nov 20095 Nov 2009
Conference number: 13

Publication series

NamemicroTAS 2009
PublisherChemical and Biological Society
Volume2

Conference

Conference13th International Conference on Micro Total Analysis Systems, µTAS 2009
Abbreviated titleMicroTAS
CountryKorea, Republic of
CityJeju Island
Period1/11/095/11/09

Keywords

  • METIS-268930
  • EWI-17150
  • IR-69427

Cite this

Beugelaar, G. B., Castillo-Fernandez, O., Castillo, O., Arundell, M., Samitier, J., van den Berg, A., & Eijkel, J. C. T. (2009). DNA transport in 20nm nanoslits by AC and DC electrical fields. In T. S. Kim, Y-S. Lee, T-D. Chung, N. L. Jeon, S. H. Lee, K-Y. Suh, J. Choo, ... Y-K. Kim (Eds.), Micro Total Analysis Systems 2009 (pp. 1518-1520). (microTAS 2009; Vol. 2). San Diego: Chemical and Biological Society.
Beugelaar, G.B. ; Castillo-Fernandez, O. ; Castillo, O. ; Arundell, M. ; Samitier, J. ; van den Berg, Albert ; Eijkel, Jan C.T. / DNA transport in 20nm nanoslits by AC and DC electrical fields. Micro Total Analysis Systems 2009. editor / T.S. Kim ; Y-S Lee ; T-D Chung ; N.L. Jeon ; S.H. Lee ; K-Y Suh ; J. Choo ; Y-K Kim. San Diego : Chemical and Biological Society, 2009. pp. 1518-1520 (microTAS 2009).
@inproceedings{92e0a7d21f094b4ba37b4a4b5d3a15ab,
title = "DNA transport in 20nm nanoslits by AC and DC electrical fields",
abstract = "We investigated the transport of λ-DNA in 20 nm nanoslits under the influence of applied AC and DC fields. At DC fields below 15 kV/m it was found that the addition of 1 kHz AC fields with maximum strengths between 10 and 200 kV/m significantly increased the mobility. At DC fields above 15 kV/m no influence of the AC fields was observed. The observed behavior indicates that steric effects rather than dielectrophoretic effects are responsible for the observed low DNA mobility in 20 nm nanoslits. The observed mobility behaviour can be explained if the DNA moves by biased reptation in the nanoslits",
keywords = "METIS-268930, EWI-17150, IR-69427",
author = "G.B. Beugelaar and O. Castillo-Fernandez and O. Castillo and M. Arundell and J. Samitier and {van den Berg}, Albert and Eijkel, {Jan C.T.}",
year = "2009",
month = "11",
day = "1",
language = "Undefined",
isbn = "978-0-9798064-2-1",
series = "microTAS 2009",
publisher = "Chemical and Biological Society",
pages = "1518--1520",
editor = "T.S. Kim and Y-S Lee and T-D Chung and N.L. Jeon and S.H. Lee and K-Y Suh and J. Choo and Y-K Kim",
booktitle = "Micro Total Analysis Systems 2009",

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Beugelaar, GB, Castillo-Fernandez, O, Castillo, O, Arundell, M, Samitier, J, van den Berg, A & Eijkel, JCT 2009, DNA transport in 20nm nanoslits by AC and DC electrical fields. in TS Kim, Y-S Lee, T-D Chung, NL Jeon, SH Lee, K-Y Suh, J Choo & Y-K Kim (eds), Micro Total Analysis Systems 2009. microTAS 2009, vol. 2, Chemical and Biological Society, San Diego, pp. 1518-1520, 13th International Conference on Micro Total Analysis Systems, µTAS 2009, Jeju Island, Korea, Republic of, 1/11/09.

DNA transport in 20nm nanoslits by AC and DC electrical fields. / Beugelaar, G.B.; Castillo-Fernandez, O.; Castillo, O.; Arundell, M.; Samitier, J.; van den Berg, Albert; Eijkel, Jan C.T.

Micro Total Analysis Systems 2009. ed. / T.S. Kim; Y-S Lee; T-D Chung; N.L. Jeon; S.H. Lee; K-Y Suh; J. Choo; Y-K Kim. San Diego : Chemical and Biological Society, 2009. p. 1518-1520 (microTAS 2009; Vol. 2).

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

TY - GEN

T1 - DNA transport in 20nm nanoslits by AC and DC electrical fields

AU - Beugelaar, G.B.

AU - Castillo-Fernandez, O.

AU - Castillo, O.

AU - Arundell, M.

AU - Samitier, J.

AU - van den Berg, Albert

AU - Eijkel, Jan C.T.

PY - 2009/11/1

Y1 - 2009/11/1

N2 - We investigated the transport of λ-DNA in 20 nm nanoslits under the influence of applied AC and DC fields. At DC fields below 15 kV/m it was found that the addition of 1 kHz AC fields with maximum strengths between 10 and 200 kV/m significantly increased the mobility. At DC fields above 15 kV/m no influence of the AC fields was observed. The observed behavior indicates that steric effects rather than dielectrophoretic effects are responsible for the observed low DNA mobility in 20 nm nanoslits. The observed mobility behaviour can be explained if the DNA moves by biased reptation in the nanoslits

AB - We investigated the transport of λ-DNA in 20 nm nanoslits under the influence of applied AC and DC fields. At DC fields below 15 kV/m it was found that the addition of 1 kHz AC fields with maximum strengths between 10 and 200 kV/m significantly increased the mobility. At DC fields above 15 kV/m no influence of the AC fields was observed. The observed behavior indicates that steric effects rather than dielectrophoretic effects are responsible for the observed low DNA mobility in 20 nm nanoslits. The observed mobility behaviour can be explained if the DNA moves by biased reptation in the nanoslits

KW - METIS-268930

KW - EWI-17150

KW - IR-69427

M3 - Conference contribution

SN - 978-0-9798064-2-1

T3 - microTAS 2009

SP - 1518

EP - 1520

BT - Micro Total Analysis Systems 2009

A2 - Kim, T.S.

A2 - Lee, Y-S

A2 - Chung, T-D

A2 - Jeon, N.L.

A2 - Lee, S.H.

A2 - Suh, K-Y

A2 - Choo, J.

A2 - Kim, Y-K

PB - Chemical and Biological Society

CY - San Diego

ER -

Beugelaar GB, Castillo-Fernandez O, Castillo O, Arundell M, Samitier J, van den Berg A et al. DNA transport in 20nm nanoslits by AC and DC electrical fields. In Kim TS, Lee Y-S, Chung T-D, Jeon NL, Lee SH, Suh K-Y, Choo J, Kim Y-K, editors, Micro Total Analysis Systems 2009. San Diego: Chemical and Biological Society. 2009. p. 1518-1520. (microTAS 2009).