DNA transport in 20nm nanoslits by AC and DC electrical fields

G.B. Beugelaar; O. Castillo-Fernandez; O. Castillo; M. Arundell; J. Samitier; Albert van den Berg; Jan C.T. Eijkel

DNA transport in 20nm nanoslits by AC and DC electrical fields

G.B. Beugelaar, O. Castillo-Fernandez, O. Castillo, M. Arundell, J. Samitier, Albert van den Berg, Jan C.T. Eijkel

Faculty of Science and Technology

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

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Abstract

We investigated the transport of λ-DNA in 20 nm nanoslits under the influence of applied AC and DC fields. At DC fields below 15 kV/m it was found that the addition of 1 kHz AC fields with maximum strengths between 10 and 200 kV/m significantly increased the mobility. At DC fields above 15 kV/m no influence of the AC fields was observed. The observed behavior indicates that steric effects rather than dielectrophoretic effects are responsible for the observed low DNA mobility in 20 nm nanoslits. The observed mobility behaviour can be explained if the DNA moves by biased reptation in the nanoslits

Original language	Undefined
Title of host publication	Micro Total Analysis Systems 2009
Editors	T.S. Kim, Y-S Lee, T-D Chung, N.L. Jeon, S.H. Lee, K-Y Suh, J. Choo, Y-K Kim
Place of Publication	San Diego
Publisher	Chemical and Biological Society
Pages	1518-1520
Number of pages	3
ISBN (Print)	978-0-9798064-2-1
Publication status	Published - 1 Nov 2009
Event	13th International Conference on Micro Total Analysis Systems, µTAS 2009 - Jeju Island, Korea, Republic of Duration: 1 Nov 2009 → 5 Nov 2009 Conference number: 13

Publication series

Name	microTAS 2009
Publisher	Chemical and Biological Society
Volume	2

Conference

Conference	13th International Conference on Micro Total Analysis Systems, µTAS 2009
Abbreviated title	MicroTAS
Country	Korea, Republic of
City	Jeju Island
Period	1/11/09 → 5/11/09

Keywords

METIS-268930
EWI-17150
IR-69427

Access to Document

http://eprints.eemcs.utwente.nl/secure2/17150/01/497-W6B_Georgette_microtas.pdf

Cite this

Beugelaar, G. B., Castillo-Fernandez, O., Castillo, O., Arundell, M., Samitier, J., van den Berg, A., & Eijkel, J. C. T. (2009). DNA transport in 20nm nanoslits by AC and DC electrical fields. In T. S. Kim, Y-S. Lee, T-D. Chung, N. L. Jeon, S. H. Lee, K-Y. Suh, J. Choo, & Y-K. Kim (Eds.), Micro Total Analysis Systems 2009 (pp. 1518-1520). (microTAS 2009; Vol. 2). Chemical and Biological Society. http://eprints.eemcs.utwente.nl/secure2/17150/01/497-W6B_Georgette_microtas.pdf

Beugelaar, G.B. ; Castillo-Fernandez, O. ; Castillo, O. ; Arundell, M. ; Samitier, J. ; van den Berg, Albert ; Eijkel, Jan C.T. / DNA transport in 20nm nanoslits by AC and DC electrical fields. Micro Total Analysis Systems 2009. editor / T.S. Kim ; Y-S Lee ; T-D Chung ; N.L. Jeon ; S.H. Lee ; K-Y Suh ; J. Choo ; Y-K Kim. San Diego : Chemical and Biological Society, 2009. pp. 1518-1520 (microTAS 2009).

@inproceedings{92e0a7d21f094b4ba37b4a4b5d3a15ab,

title = "DNA transport in 20nm nanoslits by AC and DC electrical fields",

abstract = "We investigated the transport of λ-DNA in 20 nm nanoslits under the influence of applied AC and DC fields. At DC fields below 15 kV/m it was found that the addition of 1 kHz AC fields with maximum strengths between 10 and 200 kV/m significantly increased the mobility. At DC fields above 15 kV/m no influence of the AC fields was observed. The observed behavior indicates that steric effects rather than dielectrophoretic effects are responsible for the observed low DNA mobility in 20 nm nanoslits. The observed mobility behaviour can be explained if the DNA moves by biased reptation in the nanoslits",

keywords = "METIS-268930, EWI-17150, IR-69427",

author = "G.B. Beugelaar and O. Castillo-Fernandez and O. Castillo and M. Arundell and J. Samitier and {van den Berg}, Albert and Eijkel, {Jan C.T.}",

year = "2009",

month = nov,

day = "1",

language = "Undefined",

isbn = "978-0-9798064-2-1",

series = "microTAS 2009",

publisher = "Chemical and Biological Society",

pages = "1518--1520",

editor = "T.S. Kim and Y-S Lee and T-D Chung and N.L. Jeon and S.H. Lee and K-Y Suh and J. Choo and Y-K Kim",

booktitle = "Micro Total Analysis Systems 2009",

note = "null ; Conference date: 01-11-2009 Through 05-11-2009",

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Beugelaar, GB, Castillo-Fernandez, O, Castillo, O, Arundell, M, Samitier, J, van den Berg, A & Eijkel, JCT 2009, DNA transport in 20nm nanoslits by AC and DC electrical fields. in TS Kim, Y-S Lee, T-D Chung, NL Jeon, SH Lee, K-Y Suh, J Choo & Y-K Kim (eds), Micro Total Analysis Systems 2009. microTAS 2009, vol. 2, Chemical and Biological Society, San Diego, pp. 1518-1520, 13th International Conference on Micro Total Analysis Systems, µTAS 2009, Jeju Island, Korea, Republic of, 1/11/09. <http://eprints.eemcs.utwente.nl/secure2/17150/01/497-W6B_Georgette_microtas.pdf>

DNA transport in 20nm nanoslits by AC and DC electrical fields. / Beugelaar, G.B.; Castillo-Fernandez, O.; Castillo, O.; Arundell, M.; Samitier, J.; van den Berg, Albert ; Eijkel, Jan C.T.

Micro Total Analysis Systems 2009. ed. / T.S. Kim; Y-S Lee; T-D Chung; N.L. Jeon; S.H. Lee; K-Y Suh; J. Choo; Y-K Kim. San Diego : Chemical and Biological Society, 2009. p. 1518-1520 (microTAS 2009; Vol. 2).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

TY - GEN

T1 - DNA transport in 20nm nanoslits by AC and DC electrical fields

AU - Beugelaar, G.B.

AU - Castillo-Fernandez, O.

AU - Castillo, O.

AU - Arundell, M.

AU - Samitier, J.

AU - van den Berg, Albert

AU - Eijkel, Jan C.T.

N1 - Conference code: 13

PY - 2009/11/1

Y1 - 2009/11/1

N2 - We investigated the transport of λ-DNA in 20 nm nanoslits under the influence of applied AC and DC fields. At DC fields below 15 kV/m it was found that the addition of 1 kHz AC fields with maximum strengths between 10 and 200 kV/m significantly increased the mobility. At DC fields above 15 kV/m no influence of the AC fields was observed. The observed behavior indicates that steric effects rather than dielectrophoretic effects are responsible for the observed low DNA mobility in 20 nm nanoslits. The observed mobility behaviour can be explained if the DNA moves by biased reptation in the nanoslits

AB - We investigated the transport of λ-DNA in 20 nm nanoslits under the influence of applied AC and DC fields. At DC fields below 15 kV/m it was found that the addition of 1 kHz AC fields with maximum strengths between 10 and 200 kV/m significantly increased the mobility. At DC fields above 15 kV/m no influence of the AC fields was observed. The observed behavior indicates that steric effects rather than dielectrophoretic effects are responsible for the observed low DNA mobility in 20 nm nanoslits. The observed mobility behaviour can be explained if the DNA moves by biased reptation in the nanoslits

KW - METIS-268930

KW - EWI-17150

KW - IR-69427

M3 - Conference contribution

SN - 978-0-9798064-2-1

T3 - microTAS 2009

SP - 1518

EP - 1520

BT - Micro Total Analysis Systems 2009

A2 - Kim, T.S.

A2 - Lee, Y-S

A2 - Chung, T-D

A2 - Jeon, N.L.

A2 - Lee, S.H.

A2 - Suh, K-Y

A2 - Choo, J.

A2 - Kim, Y-K

PB - Chemical and Biological Society

CY - San Diego

Y2 - 1 November 2009 through 5 November 2009

ER -