DNA transport in 20nm nanoslits by AC and DC electrical fields

G.B. Beugelaar, O. Castillo-Fernandez, O. Castillo, M. Arundell, J. Samitier, Albert van den Berg, Jan C.T. Eijkel

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

1 Downloads (Pure)

Abstract

We investigated the transport of λ-DNA in 20 nm nanoslits under the influence of applied AC and DC fields. At DC fields below 15 kV/m it was found that the addition of 1 kHz AC fields with maximum strengths between 10 and 200 kV/m significantly increased the mobility. At DC fields above 15 kV/m no influence of the AC fields was observed. The observed behavior indicates that steric effects rather than dielectrophoretic effects are responsible for the observed low DNA mobility in 20 nm nanoslits. The observed mobility behaviour can be explained if the DNA moves by biased reptation in the nanoslits
Original languageUndefined
Title of host publicationMicro Total Analysis Systems 2009
EditorsT.S. Kim, Y-S Lee, T-D Chung, N.L. Jeon, S.H. Lee, K-Y Suh, J. Choo, Y-K Kim
Place of PublicationSan Diego
PublisherChemical and Biological Society
Pages1518-1520
Number of pages3
ISBN (Print)978-0-9798064-2-1
Publication statusPublished - 1 Nov 2009
Event13th International Conference on Micro Total Analysis Systems, µTAS 2009 - Jeju Island, Korea, Republic of
Duration: 1 Nov 20095 Nov 2009
Conference number: 13

Publication series

NamemicroTAS 2009
PublisherChemical and Biological Society
Volume2

Conference

Conference13th International Conference on Micro Total Analysis Systems, µTAS 2009
Abbreviated titleMicroTAS
CountryKorea, Republic of
CityJeju Island
Period1/11/095/11/09

Keywords

  • METIS-268930
  • EWI-17150
  • IR-69427

Cite this

Beugelaar, G. B., Castillo-Fernandez, O., Castillo, O., Arundell, M., Samitier, J., van den Berg, A., & Eijkel, J. C. T. (2009). DNA transport in 20nm nanoslits by AC and DC electrical fields. In T. S. Kim, Y-S. Lee, T-D. Chung, N. L. Jeon, S. H. Lee, K-Y. Suh, J. Choo, ... Y-K. Kim (Eds.), Micro Total Analysis Systems 2009 (pp. 1518-1520). (microTAS 2009; Vol. 2). San Diego: Chemical and Biological Society.