Downscaling quantitative isotachophoresis: Limits at the sub-picoliter scale

K. G.H. Janssen, J. Li, H. T. Hoang, N. R. Tas, H. J. Van Der Linden, T. Hankemeier*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

1 Citation (Scopus)
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Abstract

Isotachophoresis (ITP), an important bioanalysis technique, was downscaled 600, 000 times in volume in a 50 nm deep channel. The chip design enabled a quantitative injection volume of 200 femtoliters. To evaluate ITP as a tool in bioanalysis for sub-cellular volumes, an academic sample was injected. The sample, consisting of pretreated yeast lysate, spiked with two fluorescently labeled amino acids (10 attomoles per amino acid) was successfully focused. ITP in 0.33 μm heigh channels showed that the amino acids were also separated, contrary to ITP in 50 nm channels, indicating a limit in separation in ITP downscaling.

Original languageEnglish
Title of host publication14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Pages1730-1732
Number of pages3
Publication statusPublished - 2010
Event14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 - Groningen, Netherlands
Duration: 3 Oct 20107 Oct 2010

Conference

Conference14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Country/TerritoryNetherlands
CityGroningen
Period3/10/107/10/10

Keywords

  • Amino acid
  • Bioanalysis
  • Femtoliter
  • Isotachophoresis
  • Nanochannel
  • Single cell analysis
  • 22/4 OA procedure

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