Downscaling quantitative isotachophoresis: Limits at the sub-picoliter scale

K. G.H. Janssen; J. Li; H. T. Hoang; N. R. Tas; H. J. Van Der Linden; T. Hankemeier

Downscaling quantitative isotachophoresis: Limits at the sub-picoliter scale

K. G.H. Janssen, J. Li, H. T. Hoang, N. R. Tas, H. J. Van Der Linden, T. Hankemeier^*

^*Corresponding author for this work

MESA+ Institute

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

1 Citation (Scopus)

Abstract

Isotachophoresis (ITP), an important bioanalysis technique, was downscaled 600, 000 times in volume in a 50 nm deep channel. The chip design enabled a quantitative injection volume of 200 femtoliters. To evaluate ITP as a tool in bioanalysis for sub-cellular volumes, an academic sample was injected. The sample, consisting of pretreated yeast lysate, spiked with two fluorescently labeled amino acids (10 attomoles per amino acid) was successfully focused. ITP in 0.33 μm heigh channels showed that the amino acids were also separated, contrary to ITP in 50 nm channels, indicating a limit in separation in ITP downscaling.

Original language	English
Title of host publication	14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Pages	1730-1732
Number of pages	3
Publication status	Published - 2010
Event	14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 - Groningen, Netherlands Duration: 3 Oct 2010 → 7 Oct 2010

Conference

Conference	14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Country/Territory	Netherlands
City	Groningen
Period	3/10/10 → 7/10/10

Keywords

Amino acid
Bioanalysis
Femtoliter
Isotachophoresis
Nanochannel
Single cell analysis
22/4 OA procedure

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Downscaling_quantitative_isotachophoresis_Limits_aFinal published version, 571 KBLicence: Taverne

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@inproceedings{3f544cbbb9b7413a9d98910da56095c1,

title = "Downscaling quantitative isotachophoresis: Limits at the sub-picoliter scale",

abstract = "Isotachophoresis (ITP), an important bioanalysis technique, was downscaled 600, 000 times in volume in a 50 nm deep channel. The chip design enabled a quantitative injection volume of 200 femtoliters. To evaluate ITP as a tool in bioanalysis for sub-cellular volumes, an academic sample was injected. The sample, consisting of pretreated yeast lysate, spiked with two fluorescently labeled amino acids (10 attomoles per amino acid) was successfully focused. ITP in 0.33 μm heigh channels showed that the amino acids were also separated, contrary to ITP in 50 nm channels, indicating a limit in separation in ITP downscaling.",

keywords = "Amino acid, Bioanalysis, Femtoliter, Isotachophoresis, Nanochannel, Single cell analysis, 22/4 OA procedure",

author = "Janssen, {K. G.H.} and J. Li and Hoang, {H. T.} and Tas, {N. R.} and {Van Der Linden}, {H. J.} and T. Hankemeier",

year = "2010",

language = "English",

isbn = "9781618390622",

pages = "1730--1732",

booktitle = "14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010",

note = "14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 ; Conference date: 03-10-2010 Through 07-10-2010",

}

Janssen, KGH, Li, J, Hoang, HT, Tas, NR, Van Der Linden, HJ & Hankemeier, T 2010, Downscaling quantitative isotachophoresis: Limits at the sub-picoliter scale. in 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010. pp. 1730-1732, 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010, Groningen, Netherlands, 3/10/10.

Downscaling quantitative isotachophoresis : Limits at the sub-picoliter scale. / Janssen, K. G.H.; Li, J.; Hoang, H. T. et al.

14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010. 2010. p. 1730-1732.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

TY - GEN

T1 - Downscaling quantitative isotachophoresis

T2 - 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010

AU - Janssen, K. G.H.

AU - Li, J.

AU - Hoang, H. T.

AU - Tas, N. R.

AU - Van Der Linden, H. J.

AU - Hankemeier, T.

PY - 2010

Y1 - 2010

N2 - Isotachophoresis (ITP), an important bioanalysis technique, was downscaled 600, 000 times in volume in a 50 nm deep channel. The chip design enabled a quantitative injection volume of 200 femtoliters. To evaluate ITP as a tool in bioanalysis for sub-cellular volumes, an academic sample was injected. The sample, consisting of pretreated yeast lysate, spiked with two fluorescently labeled amino acids (10 attomoles per amino acid) was successfully focused. ITP in 0.33 μm heigh channels showed that the amino acids were also separated, contrary to ITP in 50 nm channels, indicating a limit in separation in ITP downscaling.

AB - Isotachophoresis (ITP), an important bioanalysis technique, was downscaled 600, 000 times in volume in a 50 nm deep channel. The chip design enabled a quantitative injection volume of 200 femtoliters. To evaluate ITP as a tool in bioanalysis for sub-cellular volumes, an academic sample was injected. The sample, consisting of pretreated yeast lysate, spiked with two fluorescently labeled amino acids (10 attomoles per amino acid) was successfully focused. ITP in 0.33 μm heigh channels showed that the amino acids were also separated, contrary to ITP in 50 nm channels, indicating a limit in separation in ITP downscaling.

KW - Amino acid

KW - Bioanalysis

KW - Femtoliter

KW - Isotachophoresis

KW - Nanochannel

KW - Single cell analysis

KW - 22/4 OA procedure

UR - http://www.scopus.com/inward/record.url?scp=84884336915&partnerID=8YFLogxK

M3 - Conference contribution

AN - SCOPUS:84884336915

SN - 9781618390622

SP - 1730

EP - 1732

BT - 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010

Y2 - 3 October 2010 through 7 October 2010

ER -

Downscaling quantitative isotachophoresis: Limits at the sub-picoliter scale

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Conference

Keywords

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