Drug penetration enhancement techniques in ablative fractional laser assisted cutaneous delivery of indocyanine green

Arne A. Meesters, Marilin J. Nieboer, Mitra Almasian, Giota Georgiou, Menno A. de Rie, Rudolf M. Verdaasdonk, Albert Wolkerstorfer

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Abstract

Background and Objectives: Topical drug delivery can be increased by pretreatment of the skin with ablative fractional laser (AFXL). Several physical penetration enhancement techniques have been investigated to further improve AFXL-assisted drug delivery. This study investigated the influence of three of these techniques, namely massage, acoustic pressure wave treatment, and pressure vacuum alterations (PVP) on the distribution of the fluorescent drug indocyanine green (ICG) at different depths in the skin after topical application on AFXL pretreated skin. Materials and Methods: In ex vivo human skin, test regions were pretreated with AFXL (10,600 nm, channel depth 300 μm, channel width 120 μm, density 15%). Subsequently, ICG was applied, followed by massage, acoustic pressure wave treatment or PVP. ICG fluorescence intensity (FI) was assessed after 1, 3, and 24 hours at several depths using fluorescence photography. Results: FI was higher when using enhancement techniques compared to control (AFXL-only) up to 3 hours application time (P < 0.05). After 3 hours, mean surface FI was highest after acoustic pressure wave treatment (61.5 arbitrary units; AU), followed by massage (57.5AU) and PVP (46.9AU), respectively (for comparison: AFXL-only 31.6AU, no pretreatment 14.9AU). Comparable or higher FI was achieved already after 1 hour with enhancement techniques compared to 3–24 hours application time without. After 24 hours, no significant differences between enhancement techniques and AFXL-only were observed (P = 0.31). Conclusion: Penetration enhancement techniques, especially acoustic pressure wave treatment and massage, result in improved drug accumulation in AFXL-pretreated skin and reduce the application time needed. Lasers Surg. Med.

Original languageEnglish
Pages (from-to)709-719
Number of pages11
JournalLasers in surgery and medicine
Volume51
Issue number8
Early online date25 Mar 2019
DOIs
Publication statusPublished - Oct 2019

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Indocyanine Green
Massage
Lasers
Fluorescence
Pressure
Skin
Pharmaceutical Preparations
Photography
Therapeutics
Vacuum
Skin Tests

Keywords

  • UT-Hybrid-D
  • fractional laser
  • indocyanine green
  • topical therapy
  • drug delivery

Cite this

Meesters, A. A., Nieboer, M. J., Almasian, M., Georgiou, G., de Rie, M. A., Verdaasdonk, R. M., & Wolkerstorfer, A. (2019). Drug penetration enhancement techniques in ablative fractional laser assisted cutaneous delivery of indocyanine green. Lasers in surgery and medicine, 51(8), 709-719. https://doi.org/10.1002/lsm.23088
Meesters, Arne A. ; Nieboer, Marilin J. ; Almasian, Mitra ; Georgiou, Giota ; de Rie, Menno A. ; Verdaasdonk, Rudolf M. ; Wolkerstorfer, Albert. / Drug penetration enhancement techniques in ablative fractional laser assisted cutaneous delivery of indocyanine green. In: Lasers in surgery and medicine. 2019 ; Vol. 51, No. 8. pp. 709-719.
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title = "Drug penetration enhancement techniques in ablative fractional laser assisted cutaneous delivery of indocyanine green",
abstract = "Background and Objectives: Topical drug delivery can be increased by pretreatment of the skin with ablative fractional laser (AFXL). Several physical penetration enhancement techniques have been investigated to further improve AFXL-assisted drug delivery. This study investigated the influence of three of these techniques, namely massage, acoustic pressure wave treatment, and pressure vacuum alterations (PVP) on the distribution of the fluorescent drug indocyanine green (ICG) at different depths in the skin after topical application on AFXL pretreated skin. Materials and Methods: In ex vivo human skin, test regions were pretreated with AFXL (10,600 nm, channel depth 300 μm, channel width 120 μm, density 15{\%}). Subsequently, ICG was applied, followed by massage, acoustic pressure wave treatment or PVP. ICG fluorescence intensity (FI) was assessed after 1, 3, and 24 hours at several depths using fluorescence photography. Results: FI was higher when using enhancement techniques compared to control (AFXL-only) up to 3 hours application time (P < 0.05). After 3 hours, mean surface FI was highest after acoustic pressure wave treatment (61.5 arbitrary units; AU), followed by massage (57.5AU) and PVP (46.9AU), respectively (for comparison: AFXL-only 31.6AU, no pretreatment 14.9AU). Comparable or higher FI was achieved already after 1 hour with enhancement techniques compared to 3–24 hours application time without. After 24 hours, no significant differences between enhancement techniques and AFXL-only were observed (P = 0.31). Conclusion: Penetration enhancement techniques, especially acoustic pressure wave treatment and massage, result in improved drug accumulation in AFXL-pretreated skin and reduce the application time needed. Lasers Surg. Med.",
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Meesters, AA, Nieboer, MJ, Almasian, M, Georgiou, G, de Rie, MA, Verdaasdonk, RM & Wolkerstorfer, A 2019, 'Drug penetration enhancement techniques in ablative fractional laser assisted cutaneous delivery of indocyanine green' Lasers in surgery and medicine, vol. 51, no. 8, pp. 709-719. https://doi.org/10.1002/lsm.23088

Drug penetration enhancement techniques in ablative fractional laser assisted cutaneous delivery of indocyanine green. / Meesters, Arne A.; Nieboer, Marilin J.; Almasian, Mitra; Georgiou, Giota; de Rie, Menno A.; Verdaasdonk, Rudolf M.; Wolkerstorfer, Albert.

In: Lasers in surgery and medicine, Vol. 51, No. 8, 10.2019, p. 709-719.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Drug penetration enhancement techniques in ablative fractional laser assisted cutaneous delivery of indocyanine green

AU - Meesters, Arne A.

AU - Nieboer, Marilin J.

AU - Almasian, Mitra

AU - Georgiou, Giota

AU - de Rie, Menno A.

AU - Verdaasdonk, Rudolf M.

AU - Wolkerstorfer, Albert

N1 - Wiley deal

PY - 2019/10

Y1 - 2019/10

N2 - Background and Objectives: Topical drug delivery can be increased by pretreatment of the skin with ablative fractional laser (AFXL). Several physical penetration enhancement techniques have been investigated to further improve AFXL-assisted drug delivery. This study investigated the influence of three of these techniques, namely massage, acoustic pressure wave treatment, and pressure vacuum alterations (PVP) on the distribution of the fluorescent drug indocyanine green (ICG) at different depths in the skin after topical application on AFXL pretreated skin. Materials and Methods: In ex vivo human skin, test regions were pretreated with AFXL (10,600 nm, channel depth 300 μm, channel width 120 μm, density 15%). Subsequently, ICG was applied, followed by massage, acoustic pressure wave treatment or PVP. ICG fluorescence intensity (FI) was assessed after 1, 3, and 24 hours at several depths using fluorescence photography. Results: FI was higher when using enhancement techniques compared to control (AFXL-only) up to 3 hours application time (P < 0.05). After 3 hours, mean surface FI was highest after acoustic pressure wave treatment (61.5 arbitrary units; AU), followed by massage (57.5AU) and PVP (46.9AU), respectively (for comparison: AFXL-only 31.6AU, no pretreatment 14.9AU). Comparable or higher FI was achieved already after 1 hour with enhancement techniques compared to 3–24 hours application time without. After 24 hours, no significant differences between enhancement techniques and AFXL-only were observed (P = 0.31). Conclusion: Penetration enhancement techniques, especially acoustic pressure wave treatment and massage, result in improved drug accumulation in AFXL-pretreated skin and reduce the application time needed. Lasers Surg. Med.

AB - Background and Objectives: Topical drug delivery can be increased by pretreatment of the skin with ablative fractional laser (AFXL). Several physical penetration enhancement techniques have been investigated to further improve AFXL-assisted drug delivery. This study investigated the influence of three of these techniques, namely massage, acoustic pressure wave treatment, and pressure vacuum alterations (PVP) on the distribution of the fluorescent drug indocyanine green (ICG) at different depths in the skin after topical application on AFXL pretreated skin. Materials and Methods: In ex vivo human skin, test regions were pretreated with AFXL (10,600 nm, channel depth 300 μm, channel width 120 μm, density 15%). Subsequently, ICG was applied, followed by massage, acoustic pressure wave treatment or PVP. ICG fluorescence intensity (FI) was assessed after 1, 3, and 24 hours at several depths using fluorescence photography. Results: FI was higher when using enhancement techniques compared to control (AFXL-only) up to 3 hours application time (P < 0.05). After 3 hours, mean surface FI was highest after acoustic pressure wave treatment (61.5 arbitrary units; AU), followed by massage (57.5AU) and PVP (46.9AU), respectively (for comparison: AFXL-only 31.6AU, no pretreatment 14.9AU). Comparable or higher FI was achieved already after 1 hour with enhancement techniques compared to 3–24 hours application time without. After 24 hours, no significant differences between enhancement techniques and AFXL-only were observed (P = 0.31). Conclusion: Penetration enhancement techniques, especially acoustic pressure wave treatment and massage, result in improved drug accumulation in AFXL-pretreated skin and reduce the application time needed. Lasers Surg. Med.

KW - UT-Hybrid-D

KW - fractional laser

KW - indocyanine green

KW - topical therapy

KW - drug delivery

U2 - 10.1002/lsm.23088

DO - 10.1002/lsm.23088

M3 - Article

VL - 51

SP - 709

EP - 719

JO - Lasers in surgery and medicine

JF - Lasers in surgery and medicine

SN - 0196-8092

IS - 8

ER -