Abstract
We report a versatile platform for the production of spheroids of various dimensions, based on tumor cells only (mono-culture spheroids) or a combination of tumor cells and fibroblasts (co-culture spheroids). Spheroids prepared in microwells of different dimensions and with different cellular compositions are characterized in terms of size, cellular organization, and protein expression profiles. Following this, drug screening assays are conducted, either in situ in the production platform or in a separate microfluidic platform in which individual microtissues are isolated, trapped and exposed to model nanoparticle solutions under either static or dynamic conditions.
| Original language | English |
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| Title of host publication | 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016) |
| Publisher | The Chemical and Biological Microsystems Society |
| Pages | 45-46 |
| Number of pages | 2 |
| ISBN (Electronic) | 978-0-9798-0649-0 |
| ISBN (Print) | 978-1-5108-3416-3 |
| Publication status | Published - 2016 |
| Event | 20th International Conference on Miniaturized Systems for Chemistry and LifeSciences, µTAS 2016 - Convention Center Dublin, Dublin, Ireland Duration: 9 Oct 2016 → 13 Oct 2016 Conference number: 20 http://www.microtas2016.org/ |
Conference
| Conference | 20th International Conference on Miniaturized Systems for Chemistry and LifeSciences, µTAS 2016 |
|---|---|
| Abbreviated title | MicroTAS 2016 |
| Country/Territory | Ireland |
| City | Dublin |
| Period | 9/10/16 → 13/10/16 |
| Internet address |
Keywords
- 3D tumor spheroids
- Drug screening
- Microwell array
- Nanoparticles