Dual bio-responsive gene delivery via reducible poly(amido amine) and survivin-inducible plasmid DNA

Ran Namgung, J.H. Brumbach, Jonathan H. Brumbach, Ji Hoon Jeong, James W. Yockman, Sung Wan Kim, C. Lin, Zhiyuan Zhong, Jan Feijen, Johannes F.J. Engbersen, Won Jong Kim

Research output: Contribution to journalArticleAcademicpeer-review

14 Citations (Scopus)

Abstract

A bioreducible poly(amido amine) (SS-PAA) gene carrier, known as poly (amido-butanol) (pABOL), was used to transfect a variety of cancer and non-cancer cell lines. To obtain cancer-specific transgene expression for therapeutic efficiency in cancer treatment, we constructed survivin-inducible plasmid DNA expressing the soluble VEGF receptor, sFlt-1, downstream of the survivin promoter (pSUR-sFlt-1). Cancer-specific expression of sFlt-1 was observed in the mouse renal carcinoma (RENCA) cell line. pABOL enhanced the efficiency of gene delivery compared to traditional carriers used in the past. Thus, a dual bio-responsive gene delivery system was developed by using bioreducible p(ABOL) for enhanced intracellular gene delivery and survivin-inducible gene expression system (pSUR-sFlt-1 or pSUR-Luc reporter gene) that demonstrates increased gene expression in cancer that has advantages over current gene delivery systems
Original languageEnglish
Pages (from-to)755-764
JournalBiotechnology letters
Volume32
Issue number6
DOIs
Publication statusPublished - 2010

Keywords

  • IR-77536
  • Dual bio-responsive - Gene therapy - Reducible polymer - Survivin - Tumor cells
  • METIS-273753

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