Dual bio-responsive gene delivery via reducible poly(amido amine) and survivin-inducible plasmid DNA

Ran Namgung; J.H. Brumbach; Jonathan H. Brumbach; Ji Hoon Jeong; James W. Yockman; Sung Wan Kim; C. Lin; Zhiyuan Zhong; Jan Feijen; Johannes F.J. Engbersen; Won Jong Kim

doi:10.1007/s10529-010-0219-7

Dual bio-responsive gene delivery via reducible poly(amido amine) and survivin-inducible plasmid DNA

Ran Namgung, J.H. Brumbach, Jonathan H. Brumbach, Ji Hoon Jeong, James W. Yockman, Sung Wan Kim, C. Lin, Zhiyuan Zhong, Jan Feijen, Johannes F.J. Engbersen, Won Jong Kim

Faculty of Science and Technology

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

A bioreducible poly(amido amine) (SS-PAA) gene carrier, known as poly (amido-butanol) (pABOL), was used to transfect a variety of cancer and non-cancer cell lines. To obtain cancer-specific transgene expression for therapeutic efficiency in cancer treatment, we constructed survivin-inducible plasmid DNA expressing the soluble VEGF receptor, sFlt-1, downstream of the survivin promoter (pSUR-sFlt-1). Cancer-specific expression of sFlt-1 was observed in the mouse renal carcinoma (RENCA) cell line. pABOL enhanced the efficiency of gene delivery compared to traditional carriers used in the past. Thus, a dual bio-responsive gene delivery system was developed by using bioreducible p(ABOL) for enhanced intracellular gene delivery and survivin-inducible gene expression system (pSUR-sFlt-1 or pSUR-Luc reporter gene) that demonstrates increased gene expression in cancer that has advantages over current gene delivery systems

Original language	English
Pages (from-to)	755-764
Journal	Biotechnology letters
Volume	32
Issue number	6
DOIs	https://doi.org/10.1007/s10529-010-0219-7
Publication status	Published - 2010

Keywords

IR-77536
Dual bio-responsive - Gene therapy - Reducible polymer - Survivin - Tumor cells
METIS-273753

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/s10529-010-0219-7

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@article{2606475b17964ea6ac2c57eb6a833cc3,

title = "Dual bio-responsive gene delivery via reducible poly(amido amine) and survivin-inducible plasmid DNA",

abstract = "A bioreducible poly(amido amine) (SS-PAA) gene carrier, known as poly (amido-butanol) (pABOL), was used to transfect a variety of cancer and non-cancer cell lines. To obtain cancer-specific transgene expression for therapeutic efficiency in cancer treatment, we constructed survivin-inducible plasmid DNA expressing the soluble VEGF receptor, sFlt-1, downstream of the survivin promoter (pSUR-sFlt-1). Cancer-specific expression of sFlt-1 was observed in the mouse renal carcinoma (RENCA) cell line. pABOL enhanced the efficiency of gene delivery compared to traditional carriers used in the past. Thus, a dual bio-responsive gene delivery system was developed by using bioreducible p(ABOL) for enhanced intracellular gene delivery and survivin-inducible gene expression system (pSUR-sFlt-1 or pSUR-Luc reporter gene) that demonstrates increased gene expression in cancer that has advantages over current gene delivery systems",

keywords = "IR-77536, Dual bio-responsive - Gene therapy - Reducible polymer - Survivin - Tumor cells, METIS-273753",

author = "Ran Namgung and J.H. Brumbach and Brumbach, {Jonathan H.} and Jeong, {Ji Hoon} and Yockman, {James W.} and Kim, {Sung Wan} and C. Lin and Zhiyuan Zhong and Jan Feijen and Engbersen, {Johannes F.J.} and Kim, {Won Jong}",

year = "2010",

doi = "10.1007/s10529-010-0219-7",

language = "English",

volume = "32",

pages = "755--764",

journal = "Biotechnology letters",

issn = "0141-5492",

publisher = "Springer",

number = "6",

}

TY - JOUR

T1 - Dual bio-responsive gene delivery via reducible poly(amido amine) and survivin-inducible plasmid DNA

AU - Namgung, Ran

AU - Brumbach, J.H.

AU - Brumbach, Jonathan H.

AU - Jeong, Ji Hoon

AU - Yockman, James W.

AU - Kim, Sung Wan

AU - Lin, C.

AU - Zhong, Zhiyuan

AU - Feijen, Jan

AU - Engbersen, Johannes F.J.

AU - Kim, Won Jong

PY - 2010

Y1 - 2010

N2 - A bioreducible poly(amido amine) (SS-PAA) gene carrier, known as poly (amido-butanol) (pABOL), was used to transfect a variety of cancer and non-cancer cell lines. To obtain cancer-specific transgene expression for therapeutic efficiency in cancer treatment, we constructed survivin-inducible plasmid DNA expressing the soluble VEGF receptor, sFlt-1, downstream of the survivin promoter (pSUR-sFlt-1). Cancer-specific expression of sFlt-1 was observed in the mouse renal carcinoma (RENCA) cell line. pABOL enhanced the efficiency of gene delivery compared to traditional carriers used in the past. Thus, a dual bio-responsive gene delivery system was developed by using bioreducible p(ABOL) for enhanced intracellular gene delivery and survivin-inducible gene expression system (pSUR-sFlt-1 or pSUR-Luc reporter gene) that demonstrates increased gene expression in cancer that has advantages over current gene delivery systems

AB - A bioreducible poly(amido amine) (SS-PAA) gene carrier, known as poly (amido-butanol) (pABOL), was used to transfect a variety of cancer and non-cancer cell lines. To obtain cancer-specific transgene expression for therapeutic efficiency in cancer treatment, we constructed survivin-inducible plasmid DNA expressing the soluble VEGF receptor, sFlt-1, downstream of the survivin promoter (pSUR-sFlt-1). Cancer-specific expression of sFlt-1 was observed in the mouse renal carcinoma (RENCA) cell line. pABOL enhanced the efficiency of gene delivery compared to traditional carriers used in the past. Thus, a dual bio-responsive gene delivery system was developed by using bioreducible p(ABOL) for enhanced intracellular gene delivery and survivin-inducible gene expression system (pSUR-sFlt-1 or pSUR-Luc reporter gene) that demonstrates increased gene expression in cancer that has advantages over current gene delivery systems

KW - IR-77536

KW - Dual bio-responsive - Gene therapy - Reducible polymer - Survivin - Tumor cells

KW - METIS-273753

U2 - 10.1007/s10529-010-0219-7

DO - 10.1007/s10529-010-0219-7

M3 - Article

SN - 0141-5492

VL - 32

SP - 755

EP - 764

JO - Biotechnology letters

JF - Biotechnology letters

IS - 6

ER -

Dual bio-responsive gene delivery via reducible poly(amido amine) and survivin-inducible plasmid DNA

Abstract

Keywords

UN SDGs

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