Dual bio-responsive gene delivery via reducible poly(amido amine) and survivin-inducible plasmid DNA

Ran Namgung; J.H. Brumbach; Jonathan H. Brumbach; Ji Hoon Jeong; James W. Yockman; Sung Wan Kim; C. Lin; Zhiyuan Zhong; Jan Feijen; Johannes F.J. Engbersen; Won Jong Kim

doi:10.1007/s10529-010-0219-7

Dual bio-responsive gene delivery via reducible poly(amido amine) and survivin-inducible plasmid DNA

Ran Namgung
, J.H. Brumbach
, Jonathan H. Brumbach
, Ji Hoon Jeong
, James W. Yockman
, Sung Wan Kim
, C. Lin
, Zhiyuan Zhong
, Jan Feijen
, Johannes F.J. Engbersen
, Won Jong Kim

Faculty of Science and Technology

Research output: Contribution to journal › Article › Academic › peer-review

15 Citations (Scopus)

3 Downloads (Pure)

Abstract

A bioreducible poly(amido amine) (SS-PAA) gene carrier, known as poly (amido-butanol) (pABOL), was used to transfect a variety of cancer and non-cancer cell lines. To obtain cancer-specific transgene expression for therapeutic efficiency in cancer treatment, we constructed survivin-inducible plasmid DNA expressing the soluble VEGF receptor, sFlt-1, downstream of the survivin promoter (pSUR-sFlt-1). Cancer-specific expression of sFlt-1 was observed in the mouse renal carcinoma (RENCA) cell line. pABOL enhanced the efficiency of gene delivery compared to traditional carriers used in the past. Thus, a dual bio-responsive gene delivery system was developed by using bioreducible p(ABOL) for enhanced intracellular gene delivery and survivin-inducible gene expression system (pSUR-sFlt-1 or pSUR-Luc reporter gene) that demonstrates increased gene expression in cancer that has advantages over current gene delivery systems

Original language	English
Pages (from-to)	755-764
Journal	Biotechnology letters
Volume	32
Issue number	6
DOIs	https://doi.org/10.1007/s10529-010-0219-7
Publication status	Published - 2010

Keywords

IR-77536
Dual bio-responsive - Gene therapy - Reducible polymer - Survivin - Tumor cells
METIS-273753

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/s10529-010-0219-7

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@article{2606475b17964ea6ac2c57eb6a833cc3,

title = "Dual bio-responsive gene delivery via reducible poly(amido amine) and survivin-inducible plasmid DNA",

abstract = "A bioreducible poly(amido amine) (SS-PAA) gene carrier, known as poly (amido-butanol) (pABOL), was used to transfect a variety of cancer and non-cancer cell lines. To obtain cancer-specific transgene expression for therapeutic efficiency in cancer treatment, we constructed survivin-inducible plasmid DNA expressing the soluble VEGF receptor, sFlt-1, downstream of the survivin promoter (pSUR-sFlt-1). Cancer-specific expression of sFlt-1 was observed in the mouse renal carcinoma (RENCA) cell line. pABOL enhanced the efficiency of gene delivery compared to traditional carriers used in the past. Thus, a dual bio-responsive gene delivery system was developed by using bioreducible p(ABOL) for enhanced intracellular gene delivery and survivin-inducible gene expression system (pSUR-sFlt-1 or pSUR-Luc reporter gene) that demonstrates increased gene expression in cancer that has advantages over current gene delivery systems",

keywords = "IR-77536, Dual bio-responsive - Gene therapy - Reducible polymer - Survivin - Tumor cells, METIS-273753",

author = "Ran Namgung and J.H. Brumbach and Brumbach, \{Jonathan H.\} and Jeong, \{Ji Hoon\} and Yockman, \{James W.\} and Kim, \{Sung Wan\} and C. Lin and Zhiyuan Zhong and Jan Feijen and Engbersen, \{Johannes F.J.\} and Kim, \{Won Jong\}",

year = "2010",

doi = "10.1007/s10529-010-0219-7",

language = "English",

volume = "32",

pages = "755--764",

journal = "Biotechnology letters",

issn = "0141-5492",

publisher = "Springer Science and Business Media B.V.",

number = "6",

}

TY - JOUR

T1 - Dual bio-responsive gene delivery via reducible poly(amido amine) and survivin-inducible plasmid DNA

AU - Namgung, Ran

AU - Brumbach, J.H.

AU - Brumbach, Jonathan H.

AU - Jeong, Ji Hoon

AU - Yockman, James W.

AU - Kim, Sung Wan

AU - Lin, C.

AU - Zhong, Zhiyuan

AU - Feijen, Jan

AU - Engbersen, Johannes F.J.

AU - Kim, Won Jong

PY - 2010

Y1 - 2010

N2 - A bioreducible poly(amido amine) (SS-PAA) gene carrier, known as poly (amido-butanol) (pABOL), was used to transfect a variety of cancer and non-cancer cell lines. To obtain cancer-specific transgene expression for therapeutic efficiency in cancer treatment, we constructed survivin-inducible plasmid DNA expressing the soluble VEGF receptor, sFlt-1, downstream of the survivin promoter (pSUR-sFlt-1). Cancer-specific expression of sFlt-1 was observed in the mouse renal carcinoma (RENCA) cell line. pABOL enhanced the efficiency of gene delivery compared to traditional carriers used in the past. Thus, a dual bio-responsive gene delivery system was developed by using bioreducible p(ABOL) for enhanced intracellular gene delivery and survivin-inducible gene expression system (pSUR-sFlt-1 or pSUR-Luc reporter gene) that demonstrates increased gene expression in cancer that has advantages over current gene delivery systems

AB - A bioreducible poly(amido amine) (SS-PAA) gene carrier, known as poly (amido-butanol) (pABOL), was used to transfect a variety of cancer and non-cancer cell lines. To obtain cancer-specific transgene expression for therapeutic efficiency in cancer treatment, we constructed survivin-inducible plasmid DNA expressing the soluble VEGF receptor, sFlt-1, downstream of the survivin promoter (pSUR-sFlt-1). Cancer-specific expression of sFlt-1 was observed in the mouse renal carcinoma (RENCA) cell line. pABOL enhanced the efficiency of gene delivery compared to traditional carriers used in the past. Thus, a dual bio-responsive gene delivery system was developed by using bioreducible p(ABOL) for enhanced intracellular gene delivery and survivin-inducible gene expression system (pSUR-sFlt-1 or pSUR-Luc reporter gene) that demonstrates increased gene expression in cancer that has advantages over current gene delivery systems

KW - IR-77536

KW - Dual bio-responsive - Gene therapy - Reducible polymer - Survivin - Tumor cells

KW - METIS-273753

U2 - 10.1007/s10529-010-0219-7

DO - 10.1007/s10529-010-0219-7

M3 - Article

SN - 0141-5492

VL - 32

SP - 755

EP - 764

JO - Biotechnology letters

JF - Biotechnology letters

IS - 6

ER -

Dual bio-responsive gene delivery via reducible poly(amido amine) and survivin-inducible plasmid DNA

Abstract

Keywords

UN SDGs

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