Dual-point dual-wavelength fluorescence monitoring of DNA separation in a lab on a chip

Chaitanya Dongre, Jasper van Weerd, Nicola Bellini, Roberto Osellame, Giulio Cerullo, Rob van Weeghel, Hugo J.W.M. Hoekstra, Markus Pollnau

    Research output: Contribution to journalArticleAcademicpeer-review

    14 Citations (Scopus)
    77 Downloads (Pure)

    Abstract

    We present a simple approach in electrophoretic DNA separation and fluorescent monitoring that allows to identify the insertion or deletion of base-pairs in DNA probe molecules from genetic samples, and to perform intrinsic calibration/referencing for highly accurate DNA analysis. The principle is based on dual-point, dual-wavelength laser-induced fluorescence excitation using one or two excitation windows at the intersection of integrated waveguides and microfluidic channels in an optofluidic chip and a single, color-blind photodetector, resulting in a limit of detection of ~200 pM for single-end-labeled DNA molecules. The approach using a single excitation window is demonstrated experimentally, while the option exploiting two excitation windows is proposed theoretically.
    Original languageEnglish
    Pages (from-to)729-735
    Number of pages7
    JournalBiomedical optics express
    Volume1
    Issue number2
    DOIs
    Publication statusPublished - Sep 2010

    Keywords

    • IOMS-SNS: SENSORS
    • Optical sensing and sensors
    • Fluorescence
    • Glass waveguides
    • EC Grant Agreement nr.: FP6/034562

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