Dual-point dual-wavelength fluorescence monitoring of DNA separation in a lab on a chip

C. Dongre, Jasper van Weerd, Nicola Bellini, Roberto Osellame, Giulio Cerullo, Rob van Weeghel, Hugo Hoekstra, Markus Pollnau

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    Abstract

    We present a simple approach in electrophoretic DNA separation and fluorescent monitoring that allows to identify the insertion or deletion of base-pairs in DNA probe molecules from genetic samples, and to perform intrinsic calibration/referencing for highly accurate DNA analysis. The principle is based on dual-point, dual-wavelength laser-induced fluorescence excitation using one or two excitation windows at the intersection of integrated waveguides and microfluidic channels in an optofluidic chip and a single, color-blind photodetector, resulting in a limit of detection of ~200 pM for single-end-labeled DNA molecules. The approach using a single excitation window is demonstrated experimentally, while the option exploiting two excitation windows is proposed theoretically.
    Original languageUndefined
    Pages (from-to)729-735
    Number of pages7
    JournalBiomedical optics express
    Volume1
    Issue number2
    DOIs
    Publication statusPublished - Sep 2010

    Keywords

    • EWI-18328
    • IOMS-SNS: SENSORS
    • Optical sensing and sensors
    • IR-72873
    • Fluorescence
    • Glass waveguides
    • METIS-277421
    • EC Grant Agreement nr.: FP6/034562

    Cite this

    Dongre, C., van Weerd, J., Bellini, N., Osellame, R., Cerullo, G., van Weeghel, R., ... Pollnau, M. (2010). Dual-point dual-wavelength fluorescence monitoring of DNA separation in a lab on a chip. Biomedical optics express, 1(2), 729-735. https://doi.org/10.1364/BOE.1.000729