We present a simple approach in electrophoretic DNA separation and fluorescent monitoring that allows to identify the insertion or deletion of base-pairs in DNA probe molecules from genetic samples, and to perform intrinsic calibration/referencing for highly accurate DNA analysis. The principle is based on dual-point, dual-wavelength laser-induced fluorescence excitation using one or two excitation windows at the intersection of integrated waveguides and microfluidic channels in an optofluidic chip and a single, color-blind photodetector, resulting in a limit of detection of ~200 pM for single-end-labeled DNA molecules. The approach using a single excitation window is demonstrated experimentally, while the option exploiting two excitation windows is proposed theoretically.
- IOMS-SNS: SENSORS
- Optical sensing and sensors
- Glass waveguides
- EC Grant Agreement nr.: FP6/034562
Dongre, C., van Weerd, J., Bellini, N., Osellame, R., Cerullo, G., van Weeghel, R., ... Pollnau, M. (2010). Dual-point dual-wavelength fluorescence monitoring of DNA separation in a lab on a chip. Biomedical optics express, 1(2), 729-735. https://doi.org/10.1364/BOE.1.000729