Calbindin-D28K is suggested to play a postsynaptic role in neurotransmission and in the regulation of the intracellular Ca2+ concentration. However, it is still unclear whether calbindin- D28K has a role in the regulation of exocytosis, either as Ca2+ buffer or as Ca2+ sensor. Amperometric recordings of catecholamine exocytosis from wild-type and calbindin-D28K knockout mouse chromaffin cells reveal a strong reduction in the number of released vesicles, as well as in the amount of neurotransmitter released per fusion event in knockout cells. However, Ca2+ current recordings and Ca2+ imaging experiments, including video-rate confocal laser scanning microscopy, revealed that the intracellular Ca2+ dynamics are remarkably similar in wild-type and knockout cells. The combined results demonstrate that calbindin-D28K plays an important and dual role in exocytosis, affecting both release frequency and quantal size, apparently without strong effects on intracellular Ca2+ dynamics. Consequently, the possibility that calbindin-D28K functions not only as a Ca2+ buffer but also as a modulator of vesicular catecholamine release is discussed. Keywords: amperometry, Ca2+ dynamics, Ca2+ sensor, calcium binding proteins, exocytosis, video-rate confocal laser scanning microscopy.
- BSS-Biomechatronics and rehabilitation technology
- Ca2+ dynamics
- Ca2+ sensor
- calcium binding proteins
- video-rate confocal laser scanning microscopy