TY - JOUR
T1 - Effect of antioxidant supplementation on the total yield, oxidative stress levels and multipotency of bone marrow-derived human mesenchymal stromal cells
AU - Alves, H.A.D.C.R.
AU - Mentink-Leusink, Anouk
AU - Le, B.Q.
AU - van Blitterswijk, Clemens
AU - de Boer, Jan
N1 - Open access article
Online Ahead of Editing
PY - 2013/11/22
Y1 - 2013/11/22
N2 - Bone marrow-derived multipotent mesenchymal stromal cells (MSCs) are the most frequently investigated cell type for potential regenerative strategies because they are relatively easy to isolate and are able to differentiate into several mesenchymal lineages. Unfortunately, during ex vivo culture, MSCs present gradual loss of differentiation potential and reduced clinical efficacy. Reactive oxygen species (ROS) are associated with oxidative damage and accumulate during MSC expansion. Because ROS are believed to be involved in the loss of multipotency, we hypothesized that compounds with antioxidant activity have the capacity to scavenge ROS, prevent cellular damage and rescue culture-induced loss of multipotency. In this manuscript, we show that antioxidant supplementation can partially rescue the loss of ALP expression induced by oxidizing agents and increases the yield of hMSCs, when supplemented to a fresh bone marrow aspirate. Concomitantly, oxidative DNA damage and ROS levels in hMSCs were reduced by anti-oxidants. We conclude that anti-oxidant supplementation during MSC expansion reduces the DNA damage load and increases the MSC yield.
AB - Bone marrow-derived multipotent mesenchymal stromal cells (MSCs) are the most frequently investigated cell type for potential regenerative strategies because they are relatively easy to isolate and are able to differentiate into several mesenchymal lineages. Unfortunately, during ex vivo culture, MSCs present gradual loss of differentiation potential and reduced clinical efficacy. Reactive oxygen species (ROS) are associated with oxidative damage and accumulate during MSC expansion. Because ROS are believed to be involved in the loss of multipotency, we hypothesized that compounds with antioxidant activity have the capacity to scavenge ROS, prevent cellular damage and rescue culture-induced loss of multipotency. In this manuscript, we show that antioxidant supplementation can partially rescue the loss of ALP expression induced by oxidizing agents and increases the yield of hMSCs, when supplemented to a fresh bone marrow aspirate. Concomitantly, oxidative DNA damage and ROS levels in hMSCs were reduced by anti-oxidants. We conclude that anti-oxidant supplementation during MSC expansion reduces the DNA damage load and increases the MSC yield.
KW - METIS-289509
KW - IR-82193
U2 - 10.1089/ten.TEA.2011.0700
DO - 10.1089/ten.TEA.2011.0700
M3 - Article
SN - 1937-3341
VL - 19
SP - 928
EP - 937
JO - Tissue engineering. Part A
JF - Tissue engineering. Part A
IS - 7-8
ER -