Effect of bile absorption coefficients on the estimation of liver tissue optical properties and related implications in discriminating healthy and tumorous samples

Rami Nachabé, Daniel Evers, Daniel J. Evers, Benno H.W. Hendriks, Gerald W. Lucassen, Gerald Lucassen, Marjolein van der Voort, Jelle Wesseling, Theo J.M. Ruers

Research output: Contribution to journalArticleAcademicpeer-review

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Abstract

We investigated differences between healthy tissue and metastatic tumor from ex vivo human partial liver resections using diffuse optical spectroscopy with a fiber optic probe. We extracted various physiological and morphological parameters from the spectra. During evaluation of the residual between the measurements and a fit model based on diffusion theory, we found that bile is an additional chromophore absorbing in the visible wavelength range that was missing in our model. Consistency of the residual with the absorption spectrum of bile was noticed. An accurate measurement of the absorption coefficient of bile from various human bile samples was performed and implemented into the fit model. Having the absorption coefficient of bile as a priori knowledge in the model showed a clear improvement in terms of reducing the fitting discrepancies. The addition of this chromophore yields significantly different estimates of the amount of blood. Furthermore, the estimated bile volume fraction and reduced scattering amplitude turned out to be two main relevant discriminators between normal and metastatic liver tissues.
Original languageUndefined
Pages (from-to)600-614
JournalBiomedical optics express
Volume2
Issue number3
DOIs
Publication statusPublished - 2011

Keywords

  • METIS-280911
  • IR-89590

Cite this

Nachabé, Rami ; Evers, Daniel ; Evers, Daniel J. ; Hendriks, Benno H.W. ; Lucassen, Gerald W. ; Lucassen, Gerald ; van der Voort, Marjolein ; Wesseling, Jelle ; Ruers, Theo J.M. / Effect of bile absorption coefficients on the estimation of liver tissue optical properties and related implications in discriminating healthy and tumorous samples. In: Biomedical optics express. 2011 ; Vol. 2, No. 3. pp. 600-614.
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title = "Effect of bile absorption coefficients on the estimation of liver tissue optical properties and related implications in discriminating healthy and tumorous samples",
abstract = "We investigated differences between healthy tissue and metastatic tumor from ex vivo human partial liver resections using diffuse optical spectroscopy with a fiber optic probe. We extracted various physiological and morphological parameters from the spectra. During evaluation of the residual between the measurements and a fit model based on diffusion theory, we found that bile is an additional chromophore absorbing in the visible wavelength range that was missing in our model. Consistency of the residual with the absorption spectrum of bile was noticed. An accurate measurement of the absorption coefficient of bile from various human bile samples was performed and implemented into the fit model. Having the absorption coefficient of bile as a priori knowledge in the model showed a clear improvement in terms of reducing the fitting discrepancies. The addition of this chromophore yields significantly different estimates of the amount of blood. Furthermore, the estimated bile volume fraction and reduced scattering amplitude turned out to be two main relevant discriminators between normal and metastatic liver tissues.",
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author = "Rami Nachab{\'e} and Daniel Evers and Evers, {Daniel J.} and Hendriks, {Benno H.W.} and Lucassen, {Gerald W.} and Gerald Lucassen and {van der Voort}, Marjolein and Jelle Wesseling and Ruers, {Theo J.M.}",
year = "2011",
doi = "10.1364/BOE.2.000600",
language = "Undefined",
volume = "2",
pages = "600--614",
journal = "Biomedical optics express",
issn = "2156-7085",
publisher = "The Optical Society",
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Effect of bile absorption coefficients on the estimation of liver tissue optical properties and related implications in discriminating healthy and tumorous samples. / Nachabé, Rami; Evers, Daniel; Evers, Daniel J.; Hendriks, Benno H.W.; Lucassen, Gerald W.; Lucassen, Gerald; van der Voort, Marjolein; Wesseling, Jelle; Ruers, Theo J.M.

In: Biomedical optics express, Vol. 2, No. 3, 2011, p. 600-614.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Effect of bile absorption coefficients on the estimation of liver tissue optical properties and related implications in discriminating healthy and tumorous samples

AU - Nachabé, Rami

AU - Evers, Daniel

AU - Evers, Daniel J.

AU - Hendriks, Benno H.W.

AU - Lucassen, Gerald W.

AU - Lucassen, Gerald

AU - van der Voort, Marjolein

AU - Wesseling, Jelle

AU - Ruers, Theo J.M.

PY - 2011

Y1 - 2011

N2 - We investigated differences between healthy tissue and metastatic tumor from ex vivo human partial liver resections using diffuse optical spectroscopy with a fiber optic probe. We extracted various physiological and morphological parameters from the spectra. During evaluation of the residual between the measurements and a fit model based on diffusion theory, we found that bile is an additional chromophore absorbing in the visible wavelength range that was missing in our model. Consistency of the residual with the absorption spectrum of bile was noticed. An accurate measurement of the absorption coefficient of bile from various human bile samples was performed and implemented into the fit model. Having the absorption coefficient of bile as a priori knowledge in the model showed a clear improvement in terms of reducing the fitting discrepancies. The addition of this chromophore yields significantly different estimates of the amount of blood. Furthermore, the estimated bile volume fraction and reduced scattering amplitude turned out to be two main relevant discriminators between normal and metastatic liver tissues.

AB - We investigated differences between healthy tissue and metastatic tumor from ex vivo human partial liver resections using diffuse optical spectroscopy with a fiber optic probe. We extracted various physiological and morphological parameters from the spectra. During evaluation of the residual between the measurements and a fit model based on diffusion theory, we found that bile is an additional chromophore absorbing in the visible wavelength range that was missing in our model. Consistency of the residual with the absorption spectrum of bile was noticed. An accurate measurement of the absorption coefficient of bile from various human bile samples was performed and implemented into the fit model. Having the absorption coefficient of bile as a priori knowledge in the model showed a clear improvement in terms of reducing the fitting discrepancies. The addition of this chromophore yields significantly different estimates of the amount of blood. Furthermore, the estimated bile volume fraction and reduced scattering amplitude turned out to be two main relevant discriminators between normal and metastatic liver tissues.

KW - METIS-280911

KW - IR-89590

U2 - 10.1364/BOE.2.000600

DO - 10.1364/BOE.2.000600

M3 - Article

VL - 2

SP - 600

EP - 614

JO - Biomedical optics express

JF - Biomedical optics express

SN - 2156-7085

IS - 3

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