Effect of Microfluidic Processing on the Viability of Boar and Bull Spermatozoa

Tanja  Hamacher; Johanna Theodora Wilhelmina Berendsen; Stella-Maria Annette Kruit; Marleen L.W.J. Broekhuijse; Loes Segerink

Effect of Microfluidic Processing on the Viability of Boar and Bull Spermatozoa

Tanja Hamacher, Johanna Theodora Wilhelmina Berendsen, Stella-Maria Annette Kruit, Marleen L.W.J. Broekhuijse, Loes Segerink

Research output: Contribution to conference › Abstract › Academic

Abstract

In this study, we evaluate the impact of microfluidic processing on the viability of spermatozoa and compare shear stress in various chip configurations and connection tubing. In contrast to other microfluidic devices for cell analysis, the spermatozoa are still used after processing in our application. During this processing, the semen is exposed to shear stress which might harm the viability and functioning of the spermatozoa. Our results indicate that the impact of microfluidic processing on the viability of boar and bull spermatozoa is less than literature reported for flow cytometry and that it is comparable to the impact of conventional centrifugation.

Original language	English
Pages	907-908
Number of pages	2
Publication status	Published - 6 Oct 2020
Event	24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2020 - Virtual Event Duration: 4 Oct 2020 → 9 Oct 2020 Conference number: 24 https://microtas2020.org/

Conference

Conference	24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2020
Abbreviated title	MicroTAS 2020
City	Virtual Event
Period	4/10/20 → 9/10/20
Internet address	https://microtas2020.org/

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title = "Effect of Microfluidic Processing on the Viability of Boar and Bull Spermatozoa",

abstract = "In this study, we evaluate the impact of microfluidic processing on the viability of spermatozoa and compare shear stress in various chip configurations and connection tubing. In contrast to other microfluidic devices for cell analysis, the spermatozoa are still used after processing in our application. During this processing, the semen is exposed to shear stress which might harm the viability and functioning of the spermatozoa. Our results indicate that the impact of microfluidic processing on the viability of boar and bull spermatozoa is less than literature reported for flow cytometry and that it is comparable to the impact of conventional centrifugation.",

author = "Tanja Hamacher and Berendsen, {Johanna Theodora Wilhelmina} and Kruit, {Stella-Maria Annette} and Broekhuijse, {Marleen L.W.J.} and Loes Segerink",

year = "2020",

month = oct,

day = "6",

language = "English",

pages = "907--908",

note = "24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2020, MicroTAS 2020 ; Conference date: 04-10-2020 Through 09-10-2020",

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Effect of Microfluidic Processing on the Viability of Boar and Bull Spermatozoa. / Hamacher, Tanja ; Berendsen, Johanna Theodora Wilhelmina ; Kruit, Stella-Maria Annette et al.
2020. 907-908 Abstract from 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2020, Virtual Event.

Research output: Contribution to conference › Abstract › Academic

TY - CONF

T1 - Effect of Microfluidic Processing on the Viability of Boar and Bull Spermatozoa

AU - Hamacher, Tanja

AU - Berendsen, Johanna Theodora Wilhelmina

AU - Kruit, Stella-Maria Annette

AU - Broekhuijse, Marleen L.W.J.

AU - Segerink, Loes

N1 - Conference code: 24

PY - 2020/10/6

Y1 - 2020/10/6

N2 - In this study, we evaluate the impact of microfluidic processing on the viability of spermatozoa and compare shear stress in various chip configurations and connection tubing. In contrast to other microfluidic devices for cell analysis, the spermatozoa are still used after processing in our application. During this processing, the semen is exposed to shear stress which might harm the viability and functioning of the spermatozoa. Our results indicate that the impact of microfluidic processing on the viability of boar and bull spermatozoa is less than literature reported for flow cytometry and that it is comparable to the impact of conventional centrifugation.

AB - In this study, we evaluate the impact of microfluidic processing on the viability of spermatozoa and compare shear stress in various chip configurations and connection tubing. In contrast to other microfluidic devices for cell analysis, the spermatozoa are still used after processing in our application. During this processing, the semen is exposed to shear stress which might harm the viability and functioning of the spermatozoa. Our results indicate that the impact of microfluidic processing on the viability of boar and bull spermatozoa is less than literature reported for flow cytometry and that it is comparable to the impact of conventional centrifugation.

M3 - Abstract

SP - 907

EP - 908

T2 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2020

Y2 - 4 October 2020 through 9 October 2020

ER -

Effect of Microfluidic Processing on the Viability of Boar and Bull Spermatozoa

Abstract

Conference

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