Effect of radiation on the Notch signaling pathway in osteoblasts

Bing Yang, Quan Tang, Janine Post, Hui Zhou, Xiao-Bin Huang, Xiao-Dong Zhang, Qin Wang, Yuan-Ming Sun, Fei-Yue Fan

Research output: Contribution to journalArticleAcademicpeer-review

10 Citations (Scopus)

Abstract

Notch signaling has been shown to be important in osteoblast differentiation. Therapeutic radiation has been shown to alter the skeletal system, yet little information is available on the changes in Notch signaling in irradiated osteoblasts. The purpose of this study was to analyze the effect of radiation therapy with 2 and 4 Gy on Notch signaling in osteoblasts. In order to assess the radiation damage on osteoblast differentiation, total RNA and protein were collected three days after exposure to radiation. The effects of radiation on Notch signaling at the early and terminal stages of osteoblastic MC3T3-E1 cell differentiation was analyzed by qRT-PCR and western blot analysis. Our study applied a previously established method to induce MC3T3-E1 cell differentiation into osteoblasts and osteoblast precursors. Our results showed that the expression of Notch receptors (Notch1-4), ligands (Jagged1, Jagged2 and Delta1), target of Notch signaling (Hes1) and markers (ALP, M-CSF, RANKL and OPG) were altered following 2 and 4 Gy of irradiation. The present research did not indicate a strong relationship between Notch1 regulation and suppression of osteoblast differentiation. We found Hes1 may play a role in the radiation effect on osteoblast differentiation. Our results indicate that radiated osteoblast precursors and osteoblasts promoted osteoclast differentiation and proliferation.
Original languageEnglish
Pages (from-to)698-706
JournalInternational journal of molecular medicine
Volume31
Issue number3
DOIs
Publication statusPublished - 2013

Fingerprint

Radiation Effects
Osteoblasts
Cell Differentiation
Radiation
Notch Receptors
Macrophage Colony-Stimulating Factor
Osteoclasts
Radiotherapy
Western Blotting
RNA
Ligands
Polymerase Chain Reaction

Keywords

  • Notch signaling
  • Osteoblast
  • Radiation
  • MC3T3 cells
  • Differentiation

Cite this

Yang, Bing ; Tang, Quan ; Post, Janine ; Zhou, Hui ; Huang, Xiao-Bin ; Zhang, Xiao-Dong ; Wang, Qin ; Sun, Yuan-Ming ; Fan, Fei-Yue. / Effect of radiation on the Notch signaling pathway in osteoblasts. In: International journal of molecular medicine. 2013 ; Vol. 31, No. 3. pp. 698-706.
@article{ad95ba07d53e44b3ac14ccab8c6438ac,
title = "Effect of radiation on the Notch signaling pathway in osteoblasts",
abstract = "Notch signaling has been shown to be important in osteoblast differentiation. Therapeutic radiation has been shown to alter the skeletal system, yet little information is available on the changes in Notch signaling in irradiated osteoblasts. The purpose of this study was to analyze the effect of radiation therapy with 2 and 4 Gy on Notch signaling in osteoblasts. In order to assess the radiation damage on osteoblast differentiation, total RNA and protein were collected three days after exposure to radiation. The effects of radiation on Notch signaling at the early and terminal stages of osteoblastic MC3T3-E1 cell differentiation was analyzed by qRT-PCR and western blot analysis. Our study applied a previously established method to induce MC3T3-E1 cell differentiation into osteoblasts and osteoblast precursors. Our results showed that the expression of Notch receptors (Notch1-4), ligands (Jagged1, Jagged2 and Delta1), target of Notch signaling (Hes1) and markers (ALP, M-CSF, RANKL and OPG) were altered following 2 and 4 Gy of irradiation. The present research did not indicate a strong relationship between Notch1 regulation and suppression of osteoblast differentiation. We found Hes1 may play a role in the radiation effect on osteoblast differentiation. Our results indicate that radiated osteoblast precursors and osteoblasts promoted osteoclast differentiation and proliferation.",
keywords = "Notch signaling, Osteoblast, Radiation, MC3T3 cells, Differentiation",
author = "Bing Yang and Quan Tang and Janine Post and Hui Zhou and Xiao-Bin Huang and Xiao-Dong Zhang and Qin Wang and Yuan-Ming Sun and Fei-Yue Fan",
year = "2013",
doi = "10.3892/ijmm.2013.1255",
language = "English",
volume = "31",
pages = "698--706",
journal = "International journal of molecular medicine",
issn = "1107-3756",
publisher = "Spandidos Publications",
number = "3",

}

Yang, B, Tang, Q, Post, J, Zhou, H, Huang, X-B, Zhang, X-D, Wang, Q, Sun, Y-M & Fan, F-Y 2013, 'Effect of radiation on the Notch signaling pathway in osteoblasts', International journal of molecular medicine, vol. 31, no. 3, pp. 698-706. https://doi.org/10.3892/ijmm.2013.1255

Effect of radiation on the Notch signaling pathway in osteoblasts. / Yang, Bing; Tang, Quan; Post, Janine; Zhou, Hui; Huang, Xiao-Bin; Zhang, Xiao-Dong; Wang, Qin; Sun, Yuan-Ming; Fan, Fei-Yue.

In: International journal of molecular medicine, Vol. 31, No. 3, 2013, p. 698-706.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Effect of radiation on the Notch signaling pathway in osteoblasts

AU - Yang, Bing

AU - Tang, Quan

AU - Post, Janine

AU - Zhou, Hui

AU - Huang, Xiao-Bin

AU - Zhang, Xiao-Dong

AU - Wang, Qin

AU - Sun, Yuan-Ming

AU - Fan, Fei-Yue

PY - 2013

Y1 - 2013

N2 - Notch signaling has been shown to be important in osteoblast differentiation. Therapeutic radiation has been shown to alter the skeletal system, yet little information is available on the changes in Notch signaling in irradiated osteoblasts. The purpose of this study was to analyze the effect of radiation therapy with 2 and 4 Gy on Notch signaling in osteoblasts. In order to assess the radiation damage on osteoblast differentiation, total RNA and protein were collected three days after exposure to radiation. The effects of radiation on Notch signaling at the early and terminal stages of osteoblastic MC3T3-E1 cell differentiation was analyzed by qRT-PCR and western blot analysis. Our study applied a previously established method to induce MC3T3-E1 cell differentiation into osteoblasts and osteoblast precursors. Our results showed that the expression of Notch receptors (Notch1-4), ligands (Jagged1, Jagged2 and Delta1), target of Notch signaling (Hes1) and markers (ALP, M-CSF, RANKL and OPG) were altered following 2 and 4 Gy of irradiation. The present research did not indicate a strong relationship between Notch1 regulation and suppression of osteoblast differentiation. We found Hes1 may play a role in the radiation effect on osteoblast differentiation. Our results indicate that radiated osteoblast precursors and osteoblasts promoted osteoclast differentiation and proliferation.

AB - Notch signaling has been shown to be important in osteoblast differentiation. Therapeutic radiation has been shown to alter the skeletal system, yet little information is available on the changes in Notch signaling in irradiated osteoblasts. The purpose of this study was to analyze the effect of radiation therapy with 2 and 4 Gy on Notch signaling in osteoblasts. In order to assess the radiation damage on osteoblast differentiation, total RNA and protein were collected three days after exposure to radiation. The effects of radiation on Notch signaling at the early and terminal stages of osteoblastic MC3T3-E1 cell differentiation was analyzed by qRT-PCR and western blot analysis. Our study applied a previously established method to induce MC3T3-E1 cell differentiation into osteoblasts and osteoblast precursors. Our results showed that the expression of Notch receptors (Notch1-4), ligands (Jagged1, Jagged2 and Delta1), target of Notch signaling (Hes1) and markers (ALP, M-CSF, RANKL and OPG) were altered following 2 and 4 Gy of irradiation. The present research did not indicate a strong relationship between Notch1 regulation and suppression of osteoblast differentiation. We found Hes1 may play a role in the radiation effect on osteoblast differentiation. Our results indicate that radiated osteoblast precursors and osteoblasts promoted osteoclast differentiation and proliferation.

KW - Notch signaling

KW - Osteoblast

KW - Radiation

KW - MC3T3 cells

KW - Differentiation

U2 - 10.3892/ijmm.2013.1255

DO - 10.3892/ijmm.2013.1255

M3 - Article

VL - 31

SP - 698

EP - 706

JO - International journal of molecular medicine

JF - International journal of molecular medicine

SN - 1107-3756

IS - 3

ER -