Effect of surface morphology of poly(ïμ-caprolactone) scaffolds on adipose stem cell adhesion and proliferation

Nazely Diban-Ibrahim Gomez, Suvi Haimi, Lydia A.M. Bolhuis-Versteeg, Sandra Da Silva Teixeira, S. Miettinen, Andreas A. Poot, Dirk W. Grijpma, Dimitrios Stamatialis

Research output: Contribution to journalArticleAcademicpeer-review

4 Citations (Scopus)

Abstract

The effect of the surface morphology of flat poly(ϵ-caprolactone) (PCL) scaffolds on human adipose stem cell (hASC) adherence and proliferation was studied. During fabrication of the scaffolds by phase inversion, the employment of different non-solvents (water (W), ethanol (EtOH) or isopropanol (IPA)) led to distinct surface morphologies. It was found that PCL scaffolds fabricated using IPA as a non-solvent had a higher roughness and porosity compared to the other groups. Moreover, during culturing of hASCs under static conditions, best cell attachment, spreading and growth were observed on the PCL scaffold. Our results show the potential of PCL scaffolds prepared using IPA as a non-solvent for especially soft tissue engineering applications.
Original languageEnglish
Pages (from-to)126-132
JournalMacromolecular symposia
Volume334
Issue number1
DOIs
Publication statusPublished - 2013
Event533th WE-Heraeus-Seminar "Advanced Functional Polymers in Medicine" 2013 - Bad Honnef, Germany
Duration: 27 May 201329 May 2013

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stem cells
Cell adhesion
Cell proliferation
Scaffolds (biology)
Stem cells
Scaffolds
Surface morphology
adhesion
2-Propanol
tissue engineering
attachment
ethyl alcohol
roughness
inversions
porosity
fabrication
cells
water
Tissue engineering
Ethanol

Keywords

  • METIS-300411
  • IR-90081

Cite this

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title = "Effect of surface morphology of poly({\"i}μ-caprolactone) scaffolds on adipose stem cell adhesion and proliferation",
abstract = "The effect of the surface morphology of flat poly(ϵ-caprolactone) (PCL) scaffolds on human adipose stem cell (hASC) adherence and proliferation was studied. During fabrication of the scaffolds by phase inversion, the employment of different non-solvents (water (W), ethanol (EtOH) or isopropanol (IPA)) led to distinct surface morphologies. It was found that PCL scaffolds fabricated using IPA as a non-solvent had a higher roughness and porosity compared to the other groups. Moreover, during culturing of hASCs under static conditions, best cell attachment, spreading and growth were observed on the PCL scaffold. Our results show the potential of PCL scaffolds prepared using IPA as a non-solvent for especially soft tissue engineering applications.",
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Effect of surface morphology of poly(ïμ-caprolactone) scaffolds on adipose stem cell adhesion and proliferation. / Diban-Ibrahim Gomez, Nazely; Haimi, Suvi; Bolhuis-Versteeg, Lydia A.M.; Da Silva Teixeira, Sandra; Miettinen, S.; Poot, Andreas A.; Grijpma, Dirk W.; Stamatialis, Dimitrios.

In: Macromolecular symposia, Vol. 334, No. 1, 2013, p. 126-132.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Effect of surface morphology of poly(ïμ-caprolactone) scaffolds on adipose stem cell adhesion and proliferation

AU - Diban-Ibrahim Gomez, Nazely

AU - Haimi, Suvi

AU - Bolhuis-Versteeg, Lydia A.M.

AU - Da Silva Teixeira, Sandra

AU - Miettinen, S.

AU - Poot, Andreas A.

AU - Grijpma, Dirk W.

AU - Stamatialis, Dimitrios

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PY - 2013

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N2 - The effect of the surface morphology of flat poly(ϵ-caprolactone) (PCL) scaffolds on human adipose stem cell (hASC) adherence and proliferation was studied. During fabrication of the scaffolds by phase inversion, the employment of different non-solvents (water (W), ethanol (EtOH) or isopropanol (IPA)) led to distinct surface morphologies. It was found that PCL scaffolds fabricated using IPA as a non-solvent had a higher roughness and porosity compared to the other groups. Moreover, during culturing of hASCs under static conditions, best cell attachment, spreading and growth were observed on the PCL scaffold. Our results show the potential of PCL scaffolds prepared using IPA as a non-solvent for especially soft tissue engineering applications.

AB - The effect of the surface morphology of flat poly(ϵ-caprolactone) (PCL) scaffolds on human adipose stem cell (hASC) adherence and proliferation was studied. During fabrication of the scaffolds by phase inversion, the employment of different non-solvents (water (W), ethanol (EtOH) or isopropanol (IPA)) led to distinct surface morphologies. It was found that PCL scaffolds fabricated using IPA as a non-solvent had a higher roughness and porosity compared to the other groups. Moreover, during culturing of hASCs under static conditions, best cell attachment, spreading and growth were observed on the PCL scaffold. Our results show the potential of PCL scaffolds prepared using IPA as a non-solvent for especially soft tissue engineering applications.

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