Effects of charge density and hydrophobicity: acetylation versus benzolation of amino butyl SS-PAAS for gene delivery

Martin Piest; Johan F.J. Engbersen

doi:10.1016/j.jconrel.2010.07.068

Effects of charge density and hydrophobicity: acetylation versus benzolation of amino butyl SS-PAAS for gene delivery

Martin Piest, Johan F.J. Engbersen

Research output: Contribution to journal › Meeting Abstract › Academic

4 Citations (Scopus)

Abstract

High densities of positive charge in polymeric vectors not only result in efficient DNA condensation and particle uptake, but also induce membrane disruption, generally resulting in undesired high cytotoxicity. We previously developed a class of bioreducible poly(amido amine)s (SS-PAA)s that are significantly less toxic than PEI. In this paper we report the effect of different degrees of acetylation or benzoylation of pendant butylamine side groups in an SS-PAA. It was found that the introduction of the lipophilic benzoyl group results in a higher transfection efficiency.

Original language	English
Pages (from-to)	e92-e94
Journal	Journal of controlled release
Volume	148
Issue number	1
DOIs	https://doi.org/10.1016/j.jconrel.2010.07.068
Publication status	Published - 7 Apr 2010
Event	11th European Symposium on Controlled Drug Delivery, ESCDD 2010 - Hotel Zuiderduin, Egmond aan Zee (NL), Netherlands Duration: 7 Apr 2010 → 9 Apr 2010 Conference number: 11

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title = "Effects of charge density and hydrophobicity: acetylation versus benzolation of amino butyl SS-PAAS for gene delivery",

abstract = "High densities of positive charge in polymeric vectors not only result in efficient DNA condensation and particle uptake, but also induce membrane disruption, generally resulting in undesired high cytotoxicity. We previously developed a class of bioreducible poly(amido amine)s (SS-PAA)s that are significantly less toxic than PEI. In this paper we report the effect of different degrees of acetylation or benzoylation of pendant butylamine side groups in an SS-PAA. It was found that the introduction of the lipophilic benzoyl group results in a higher transfection efficiency.",

author = "Martin Piest and Engbersen, {Johan F.J.}",

year = "2010",

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doi = "10.1016/j.jconrel.2010.07.068",

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TY - JOUR

T1 - Effects of charge density and hydrophobicity

T2 - 11th European Symposium on Controlled Drug Delivery, ESCDD 2010

AU - Piest, Martin

AU - Engbersen, Johan F.J.

N1 - Conference code: 11

PY - 2010/4/7

Y1 - 2010/4/7

N2 - High densities of positive charge in polymeric vectors not only result in efficient DNA condensation and particle uptake, but also induce membrane disruption, generally resulting in undesired high cytotoxicity. We previously developed a class of bioreducible poly(amido amine)s (SS-PAA)s that are significantly less toxic than PEI. In this paper we report the effect of different degrees of acetylation or benzoylation of pendant butylamine side groups in an SS-PAA. It was found that the introduction of the lipophilic benzoyl group results in a higher transfection efficiency.

AB - High densities of positive charge in polymeric vectors not only result in efficient DNA condensation and particle uptake, but also induce membrane disruption, generally resulting in undesired high cytotoxicity. We previously developed a class of bioreducible poly(amido amine)s (SS-PAA)s that are significantly less toxic than PEI. In this paper we report the effect of different degrees of acetylation or benzoylation of pendant butylamine side groups in an SS-PAA. It was found that the introduction of the lipophilic benzoyl group results in a higher transfection efficiency.

U2 - 10.1016/j.jconrel.2010.07.068

DO - 10.1016/j.jconrel.2010.07.068

M3 - Meeting Abstract

VL - 148

SP - e92-e94

JO - Journal of controlled release

JF - Journal of controlled release

SN - 0168-3659

IS - 1

Y2 - 7 April 2010 through 9 April 2010

ER -