Effects of fulvestrant alone or combined with different steroids in human breast cancer cells

G.H. Jansen; H.R. Franke; F. Wolbers; M. Brinkhuis; M. Brinkhuis; I. Vermes

doi:10.1080/13697130802232500

Effects of fulvestrant alone or combined with different steroids in human breast cancer cells

G.H. Jansen, H.R. Franke, F. Wolbers, M. Brinkhuis, M. Brinkhuis, I. Vermes

Research output: Contribution to journal › Article › Academic › peer-review

4 Citations (Scopus)

Abstract

Objectives Fulvestrant is an estrogen receptor (ER) antagonist that binds, blocks and degrades the estrogen receptor and is currently used in adjuvant treatment in postmenopausal women with ER-positive breast cancer as an alternative for tamoxifen. As an antagonist, it may induce or aggravate climacteric symptoms. In order to alleviate these symptoms, one could consider hormone therapy. The objective of this study was to analyze the effect of fulvestrant alone or in combination with different steroids in human breast cancer cells invitro, and to demonstrate whether these steroids will compromise the efficacy of fulvestrant in ER-positive breast cancer cells. Methods We performed experiments invitro with various hormone therapy preparations (estradiol (E2), dihydrodydrogesterone (DHD) and tibolone) at a concentration of 10(-6) mol/l alone or combined with fulvestrant in different breast cancer cell lines, ER-positive and ER-negative. After an incubation of 144h, proliferation and apoptosis were measured. The first was measured by quantification of the expression of cyclin D1 mRNA, the latter by the Nicoletti fragmentation assay. Results This invitro study revealed clear differences in results when various hormone therapy preparations, alone or combined with fulvestrant, are added to ER-positive and ER-negative breast cancer cell lines. Conclusions Our study demonstrated that fulvestrant, an ER antagonist used in the treatment of ER-positive breast cancer, combined with E2 and DHD or in combination with tibolone, is not compromised in its efficacy in inducing apoptosis in ER-positive breast cancer cell lines invitro.

Original language	Undefined
Article number	10.1080/13697130802232500
Pages (from-to)	315-321
Number of pages	7
Journal	Climacteric
Volume	11
Issue number	4
DOIs	https://doi.org/10.1080/13697130802232500
Publication status	Published - Oct 2008

Keywords

EWI-14753
IR-62651
METIS-255962

Cite this

Jansen, G. H., Franke, H. R., Wolbers, F., Brinkhuis, M., Brinkhuis, M., & Vermes, I. (2008). Effects of fulvestrant alone or combined with different steroids in human breast cancer cells. Climacteric, 11(4), 315-321. [10.1080/13697130802232500]. https://doi.org/10.1080/13697130802232500

Jansen, G.H. ; Franke, H.R. ; Wolbers, F. ; Brinkhuis, M. ; Brinkhuis, M. ; Vermes, I. / Effects of fulvestrant alone or combined with different steroids in human breast cancer cells. In: Climacteric. 2008 ; Vol. 11, No. 4. pp. 315-321.

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abstract = "Objectives Fulvestrant is an estrogen receptor (ER) antagonist that binds, blocks and degrades the estrogen receptor and is currently used in adjuvant treatment in postmenopausal women with ER-positive breast cancer as an alternative for tamoxifen. As an antagonist, it may induce or aggravate climacteric symptoms. In order to alleviate these symptoms, one could consider hormone therapy. The objective of this study was to analyze the effect of fulvestrant alone or in combination with different steroids in human breast cancer cells invitro, and to demonstrate whether these steroids will compromise the efficacy of fulvestrant in ER-positive breast cancer cells. Methods We performed experiments invitro with various hormone therapy preparations (estradiol (E2), dihydrodydrogesterone (DHD) and tibolone) at a concentration of 10(-6) mol/l alone or combined with fulvestrant in different breast cancer cell lines, ER-positive and ER-negative. After an incubation of 144h, proliferation and apoptosis were measured. The first was measured by quantification of the expression of cyclin D1 mRNA, the latter by the Nicoletti fragmentation assay. Results This invitro study revealed clear differences in results when various hormone therapy preparations, alone or combined with fulvestrant, are added to ER-positive and ER-negative breast cancer cell lines. Conclusions Our study demonstrated that fulvestrant, an ER antagonist used in the treatment of ER-positive breast cancer, combined with E2 and DHD or in combination with tibolone, is not compromised in its efficacy in inducing apoptosis in ER-positive breast cancer cell lines invitro.",

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Jansen, GH, Franke, HR, Wolbers, F, Brinkhuis, M, Brinkhuis, M & Vermes, I 2008, 'Effects of fulvestrant alone or combined with different steroids in human breast cancer cells', Climacteric, vol. 11, no. 4, 10.1080/13697130802232500, pp. 315-321. https://doi.org/10.1080/13697130802232500

Effects of fulvestrant alone or combined with different steroids in human breast cancer cells. / Jansen, G.H.; Franke, H.R.; Wolbers, F.; Brinkhuis, M.; Brinkhuis, M.; Vermes, I.

In: Climacteric, Vol. 11, No. 4, 10.1080/13697130802232500, 10.2008, p. 315-321.

Research output: Contribution to journal › Article › Academic › peer-review

TY - JOUR

T1 - Effects of fulvestrant alone or combined with different steroids in human breast cancer cells

AU - Jansen, G.H.

AU - Franke, H.R.

AU - Wolbers, F.

AU - Brinkhuis, M.

AU - Vermes, I.

N1 - 10.1080/13697130802232500

PY - 2008/10

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N2 - Objectives Fulvestrant is an estrogen receptor (ER) antagonist that binds, blocks and degrades the estrogen receptor and is currently used in adjuvant treatment in postmenopausal women with ER-positive breast cancer as an alternative for tamoxifen. As an antagonist, it may induce or aggravate climacteric symptoms. In order to alleviate these symptoms, one could consider hormone therapy. The objective of this study was to analyze the effect of fulvestrant alone or in combination with different steroids in human breast cancer cells invitro, and to demonstrate whether these steroids will compromise the efficacy of fulvestrant in ER-positive breast cancer cells. Methods We performed experiments invitro with various hormone therapy preparations (estradiol (E2), dihydrodydrogesterone (DHD) and tibolone) at a concentration of 10(-6) mol/l alone or combined with fulvestrant in different breast cancer cell lines, ER-positive and ER-negative. After an incubation of 144h, proliferation and apoptosis were measured. The first was measured by quantification of the expression of cyclin D1 mRNA, the latter by the Nicoletti fragmentation assay. Results This invitro study revealed clear differences in results when various hormone therapy preparations, alone or combined with fulvestrant, are added to ER-positive and ER-negative breast cancer cell lines. Conclusions Our study demonstrated that fulvestrant, an ER antagonist used in the treatment of ER-positive breast cancer, combined with E2 and DHD or in combination with tibolone, is not compromised in its efficacy in inducing apoptosis in ER-positive breast cancer cell lines invitro.

AB - Objectives Fulvestrant is an estrogen receptor (ER) antagonist that binds, blocks and degrades the estrogen receptor and is currently used in adjuvant treatment in postmenopausal women with ER-positive breast cancer as an alternative for tamoxifen. As an antagonist, it may induce or aggravate climacteric symptoms. In order to alleviate these symptoms, one could consider hormone therapy. The objective of this study was to analyze the effect of fulvestrant alone or in combination with different steroids in human breast cancer cells invitro, and to demonstrate whether these steroids will compromise the efficacy of fulvestrant in ER-positive breast cancer cells. Methods We performed experiments invitro with various hormone therapy preparations (estradiol (E2), dihydrodydrogesterone (DHD) and tibolone) at a concentration of 10(-6) mol/l alone or combined with fulvestrant in different breast cancer cell lines, ER-positive and ER-negative. After an incubation of 144h, proliferation and apoptosis were measured. The first was measured by quantification of the expression of cyclin D1 mRNA, the latter by the Nicoletti fragmentation assay. Results This invitro study revealed clear differences in results when various hormone therapy preparations, alone or combined with fulvestrant, are added to ER-positive and ER-negative breast cancer cell lines. Conclusions Our study demonstrated that fulvestrant, an ER antagonist used in the treatment of ER-positive breast cancer, combined with E2 and DHD or in combination with tibolone, is not compromised in its efficacy in inducing apoptosis in ER-positive breast cancer cell lines invitro.

KW - EWI-14753

KW - IR-62651

KW - METIS-255962

U2 - 10.1080/13697130802232500

DO - 10.1080/13697130802232500

M3 - Article

VL - 11

SP - 315

EP - 321

JO - Climacteric

JF - Climacteric

SN - 1369-7137