Effects of in vitro chondrogenic priming time of bone-marrow-derived mesenchymal stromal cells on in vivo endochondral bone formation

Wanxun Yang, Sanne K. Both, Gerjo J.V.M. van Osch, Yining Wang, John A. Jansen, Fang Yang

Research output: Contribution to journalArticleAcademicpeer-review

19 Citations (Scopus)

Abstract

Recapitulation of endochondral ossification leads to a new concept of bone tissue engineering via a cartilage intermediate as an osteoinductive template. In this study, we aimed to investigate the influence of in vitro chondrogenic priming time for the creation of cartilage template on the in vivo endochondral bone formation both qualitatively and quantitatively. To this end, rat bone-marrow-derived mesenchymal stromal cells (MSCs) were seeded onto two scaffolds with distinguished features: a fibrous poly(lactic-co-glycolic acid)/poly(ε-caprolactone) electrospun scaffold (PLGA/PCL) and a porous hydroxyapatite/tricalcium phosphate composite (HA/TCP). The constructs were then chondrogenically differentiated for 2, 3 and 4 weeks in vitro, followed by subcutaneous implantation in vivo for up to 8 weeks. A longer chondrogenic priming time resulted in a significantly increased amount and homogeneous deposition of the cartilage matrix on both the PLGA/PCL and HA/TCP scaffolds in vitro. In vivo, all implanted constructs gave rise to endochondral bone formation, whereas the bone volume was not affected by the length of priming time. An unpolarized woven bone-like structure, with significant amounts of cartilage remaining, was generated in fibrous PLGA/PCL scaffolds, while porous HA/TCP scaffolds supported progressive lamellar-like bone formation with mature bone marrow development. These data suggest that, by utilizing a chondrogenically differentiated MSC-scaffold construct as cartilage template, 2 weeks of in vitro priming time is sufficient to generate a substantial amount of vascularized endochondral bone in vivo. The structure of the bone depends on the chemical and structural cues provided by the scaffold design.

Original languageEnglish
Pages (from-to)254-265
Number of pages12
JournalActa biomaterialia
Volume13
DOIs
Publication statusPublished - 1 Feb 2015
Externally publishedYes

Fingerprint

Mesenchymal Stromal Cells
Osteogenesis
Cartilage
Scaffolds
Bone
Bone Marrow
Bone and Bones
Durapatite
Hydroxyapatite
Phosphates
Bone Development
Tissue Engineering
Cues
Acids
Composite materials
In Vitro Techniques
Tissue engineering
polycaprolactone
tricalcium phosphate
polylactic acid-polyglycolic acid copolymer

Keywords

  • Bone regeneration
  • Chondrogenic priming
  • Endochondral bone formation
  • Mesenchymal stromal cells
  • Scaffold

Cite this

Yang, Wanxun ; Both, Sanne K. ; van Osch, Gerjo J.V.M. ; Wang, Yining ; Jansen, John A. ; Yang, Fang. / Effects of in vitro chondrogenic priming time of bone-marrow-derived mesenchymal stromal cells on in vivo endochondral bone formation. In: Acta biomaterialia. 2015 ; Vol. 13. pp. 254-265.
@article{ce57afab390a488baa3e1f90f3bdf980,
title = "Effects of in vitro chondrogenic priming time of bone-marrow-derived mesenchymal stromal cells on in vivo endochondral bone formation",
abstract = "Recapitulation of endochondral ossification leads to a new concept of bone tissue engineering via a cartilage intermediate as an osteoinductive template. In this study, we aimed to investigate the influence of in vitro chondrogenic priming time for the creation of cartilage template on the in vivo endochondral bone formation both qualitatively and quantitatively. To this end, rat bone-marrow-derived mesenchymal stromal cells (MSCs) were seeded onto two scaffolds with distinguished features: a fibrous poly(lactic-co-glycolic acid)/poly(ε-caprolactone) electrospun scaffold (PLGA/PCL) and a porous hydroxyapatite/tricalcium phosphate composite (HA/TCP). The constructs were then chondrogenically differentiated for 2, 3 and 4 weeks in vitro, followed by subcutaneous implantation in vivo for up to 8 weeks. A longer chondrogenic priming time resulted in a significantly increased amount and homogeneous deposition of the cartilage matrix on both the PLGA/PCL and HA/TCP scaffolds in vitro. In vivo, all implanted constructs gave rise to endochondral bone formation, whereas the bone volume was not affected by the length of priming time. An unpolarized woven bone-like structure, with significant amounts of cartilage remaining, was generated in fibrous PLGA/PCL scaffolds, while porous HA/TCP scaffolds supported progressive lamellar-like bone formation with mature bone marrow development. These data suggest that, by utilizing a chondrogenically differentiated MSC-scaffold construct as cartilage template, 2 weeks of in vitro priming time is sufficient to generate a substantial amount of vascularized endochondral bone in vivo. The structure of the bone depends on the chemical and structural cues provided by the scaffold design.",
keywords = "Bone regeneration, Chondrogenic priming, Endochondral bone formation, Mesenchymal stromal cells, Scaffold",
author = "Wanxun Yang and Both, {Sanne K.} and {van Osch}, {Gerjo J.V.M.} and Yining Wang and Jansen, {John A.} and Fang Yang",
year = "2015",
month = "2",
day = "1",
doi = "10.1016/j.actbio.2014.11.029",
language = "English",
volume = "13",
pages = "254--265",
journal = "Acta biomaterialia",
issn = "1742-7061",
publisher = "Elsevier",

}

Effects of in vitro chondrogenic priming time of bone-marrow-derived mesenchymal stromal cells on in vivo endochondral bone formation. / Yang, Wanxun; Both, Sanne K.; van Osch, Gerjo J.V.M.; Wang, Yining; Jansen, John A.; Yang, Fang.

In: Acta biomaterialia, Vol. 13, 01.02.2015, p. 254-265.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Effects of in vitro chondrogenic priming time of bone-marrow-derived mesenchymal stromal cells on in vivo endochondral bone formation

AU - Yang, Wanxun

AU - Both, Sanne K.

AU - van Osch, Gerjo J.V.M.

AU - Wang, Yining

AU - Jansen, John A.

AU - Yang, Fang

PY - 2015/2/1

Y1 - 2015/2/1

N2 - Recapitulation of endochondral ossification leads to a new concept of bone tissue engineering via a cartilage intermediate as an osteoinductive template. In this study, we aimed to investigate the influence of in vitro chondrogenic priming time for the creation of cartilage template on the in vivo endochondral bone formation both qualitatively and quantitatively. To this end, rat bone-marrow-derived mesenchymal stromal cells (MSCs) were seeded onto two scaffolds with distinguished features: a fibrous poly(lactic-co-glycolic acid)/poly(ε-caprolactone) electrospun scaffold (PLGA/PCL) and a porous hydroxyapatite/tricalcium phosphate composite (HA/TCP). The constructs were then chondrogenically differentiated for 2, 3 and 4 weeks in vitro, followed by subcutaneous implantation in vivo for up to 8 weeks. A longer chondrogenic priming time resulted in a significantly increased amount and homogeneous deposition of the cartilage matrix on both the PLGA/PCL and HA/TCP scaffolds in vitro. In vivo, all implanted constructs gave rise to endochondral bone formation, whereas the bone volume was not affected by the length of priming time. An unpolarized woven bone-like structure, with significant amounts of cartilage remaining, was generated in fibrous PLGA/PCL scaffolds, while porous HA/TCP scaffolds supported progressive lamellar-like bone formation with mature bone marrow development. These data suggest that, by utilizing a chondrogenically differentiated MSC-scaffold construct as cartilage template, 2 weeks of in vitro priming time is sufficient to generate a substantial amount of vascularized endochondral bone in vivo. The structure of the bone depends on the chemical and structural cues provided by the scaffold design.

AB - Recapitulation of endochondral ossification leads to a new concept of bone tissue engineering via a cartilage intermediate as an osteoinductive template. In this study, we aimed to investigate the influence of in vitro chondrogenic priming time for the creation of cartilage template on the in vivo endochondral bone formation both qualitatively and quantitatively. To this end, rat bone-marrow-derived mesenchymal stromal cells (MSCs) were seeded onto two scaffolds with distinguished features: a fibrous poly(lactic-co-glycolic acid)/poly(ε-caprolactone) electrospun scaffold (PLGA/PCL) and a porous hydroxyapatite/tricalcium phosphate composite (HA/TCP). The constructs were then chondrogenically differentiated for 2, 3 and 4 weeks in vitro, followed by subcutaneous implantation in vivo for up to 8 weeks. A longer chondrogenic priming time resulted in a significantly increased amount and homogeneous deposition of the cartilage matrix on both the PLGA/PCL and HA/TCP scaffolds in vitro. In vivo, all implanted constructs gave rise to endochondral bone formation, whereas the bone volume was not affected by the length of priming time. An unpolarized woven bone-like structure, with significant amounts of cartilage remaining, was generated in fibrous PLGA/PCL scaffolds, while porous HA/TCP scaffolds supported progressive lamellar-like bone formation with mature bone marrow development. These data suggest that, by utilizing a chondrogenically differentiated MSC-scaffold construct as cartilage template, 2 weeks of in vitro priming time is sufficient to generate a substantial amount of vascularized endochondral bone in vivo. The structure of the bone depends on the chemical and structural cues provided by the scaffold design.

KW - Bone regeneration

KW - Chondrogenic priming

KW - Endochondral bone formation

KW - Mesenchymal stromal cells

KW - Scaffold

UR - http://www.scopus.com/inward/record.url?scp=84920732035&partnerID=8YFLogxK

U2 - 10.1016/j.actbio.2014.11.029

DO - 10.1016/j.actbio.2014.11.029

M3 - Article

VL - 13

SP - 254

EP - 265

JO - Acta biomaterialia

JF - Acta biomaterialia

SN - 1742-7061

ER -