Effects of Polyelectrolyte Complex Micelles and Their Components on the Enzymatic Activity of Lipase

Saskia Lindhoud (Corresponding Author), Willem Norde, Martien A. Cohen Stuart

Research output: Contribution to journalArticleAcademic

35 Citations (Scopus)

Abstract

The enzymatic activity of Hl-lipase embedded in complexes of poly-2-methylvinylpyridinium-co-poly(ethylene oxide) (P2MVP41−PEO205) and poly(acrylic acid)(PAA139) is studied as a function of the PAA139 + P2MVP41−PEO205 complex composition. The measurements revealed that there are several factors that influence the enzymatic activity. When incorporated in micelles, the activity of lipase is increased, which suggests that the micelles favor the active state. The activity may further increase because the substrate tends to accumulate to the micelles. It is found that the presence of PAA139 alone also increases the enzymatic activity somewhat. Increasing of the ionic strength decreases the enzymatic activity in all systems. However, at ionic strengths where the micelles are disintegrated (>0.5 M), the activity of lipase in the presence of both polyelectrolytes is still higher than the activity of free lipase. At 0.7 M NaCl it was found that lipase in the presence of (just) P2MVP41−PEO205 is more active than lipase without this additive.
Original languageEnglish
Pages (from-to)9802-9808
Number of pages7
JournalLangmuir
Volume26
Issue number12
DOIs
Publication statusPublished - 2010
Externally publishedYes

Keywords

  • IR-99766

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