Effects of scaffold architecture on cranial bone healing

A. Berner, M.A. Woodruff, C.X.F. Lam, M.T. Arafat, S. Saifzadeh, R. Steck, J. Ren, M. Nerlich, A.K. Ekaputra, I. Gibson, D.W. Hutmacher*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

38 Citations (Scopus)
10 Downloads (Pure)


In the present study, polycaprolactone-tricalcium phosphate (PCL/TCP) scaffolds with two different fibre laydown patterns, which were coated with hydroxyapatite and gelatine, were used as an approach for optimizing bone regeneration in a critical-sized calvarial defect. After 12 weeks, bone regeneration was quantified using microcomputed tomography (micro-CT) analysis, biomechanical testing, and histological evaluation. Notably, the experimental groups with coated scaffolds showed lower bone formation and lower biomechanical properties within the defect compared to the uncoated scaffolds. Surprisingly, the different laydown pattern of the fibres resulted in different bone formation and biomechanical properties: the 0 /60 /120 scaffolds revealed lower bone formation and biomechanical properties compared to the 0 /90 scaffolds in all the experimental groups. Therefore, future bone regeneration strategies utilizing scaffolds should consider scaffold architecture as an important factor during the scaffold optimization stages in order to move closer to a clinical application.

Original languageEnglish
Pages (from-to)506-513
Number of pages8
JournalInternational Journal of Oral and Maxillofacial Surgery
Issue number4
Publication statusPublished - Apr 2014
Externally publishedYes


  • Bone tissue engineering
  • Laydown pattern
  • Polycaprolactone
  • Rat skull defect
  • Scaffolds


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