TY - JOUR
T1 - Effects of scaffold architecture on cranial bone healing
AU - Berner, A.
AU - Woodruff, M.A.
AU - Lam, C.X.F.
AU - Arafat, M.T.
AU - Saifzadeh, S.
AU - Steck, R.
AU - Ren, J.
AU - Nerlich, M.
AU - Ekaputra, A.K.
AU - Gibson, I.
AU - Hutmacher, D.W.
PY - 2014/4
Y1 - 2014/4
N2 - In the present study, polycaprolactone-tricalcium phosphate (PCL/TCP) scaffolds with two different fibre laydown patterns, which were coated with hydroxyapatite and gelatine, were used as an approach for optimizing bone regeneration in a critical-sized calvarial defect. After 12 weeks, bone regeneration was quantified using microcomputed tomography (micro-CT) analysis, biomechanical testing, and histological evaluation. Notably, the experimental groups with coated scaffolds showed lower bone formation and lower biomechanical properties within the defect compared to the uncoated scaffolds. Surprisingly, the different laydown pattern of the fibres resulted in different bone formation and biomechanical properties: the 0 /60 /120 scaffolds revealed lower bone formation and biomechanical properties compared to the 0 /90 scaffolds in all the experimental groups. Therefore, future bone regeneration strategies utilizing scaffolds should consider scaffold architecture as an important factor during the scaffold optimization stages in order to move closer to a clinical application.
AB - In the present study, polycaprolactone-tricalcium phosphate (PCL/TCP) scaffolds with two different fibre laydown patterns, which were coated with hydroxyapatite and gelatine, were used as an approach for optimizing bone regeneration in a critical-sized calvarial defect. After 12 weeks, bone regeneration was quantified using microcomputed tomography (micro-CT) analysis, biomechanical testing, and histological evaluation. Notably, the experimental groups with coated scaffolds showed lower bone formation and lower biomechanical properties within the defect compared to the uncoated scaffolds. Surprisingly, the different laydown pattern of the fibres resulted in different bone formation and biomechanical properties: the 0 /60 /120 scaffolds revealed lower bone formation and biomechanical properties compared to the 0 /90 scaffolds in all the experimental groups. Therefore, future bone regeneration strategies utilizing scaffolds should consider scaffold architecture as an important factor during the scaffold optimization stages in order to move closer to a clinical application.
KW - Bone tissue engineering
KW - Laydown pattern
KW - Polycaprolactone
KW - Rat skull defect
KW - Scaffolds
UR - http://www.scopus.com/inward/record.url?scp=84896708534&partnerID=8YFLogxK
U2 - 10.1016/j.ijom.2013.05.008
DO - 10.1016/j.ijom.2013.05.008
M3 - Article
C2 - 24183512
AN - SCOPUS:84896708534
SN - 0901-5027
VL - 43
SP - 506
EP - 513
JO - International Journal of Oral and Maxillofacial Surgery
JF - International Journal of Oral and Maxillofacial Surgery
IS - 4
ER -