Electrical simulations and experimental results of 4-electrode AC transepithelial electrical resistance measurements in gut-on-a-chip

Marinke van der Helm, Olivier Henry, Tiama Hamkins-Indik, Michael Cronce, William Leineweber, Mathieu Odijk, Andries van der Meer, Jan Eijkel, Donald E. Ingber, Albert van den Berg, Loes Segerink

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Abstract

Transepithelial electrical resistance (TEER) measurements are often used in organs-on-chips to monitor the barrier tightness of e.g. gut epithelium. Here, we present a chip with four integrated electrodes and a four-terminal alternating current (AC) measurement protocol to perform TEER measurements. The resulting impedance spectra are interpreted using electrical simulations, which include the chip with microfluidic channels, the four electrodes, the AC measurement protocol and the intestinal barrier cultured inside the device, which is modelled as a flat monolayer or as tissue with villi. Eventually, these simulations will be used to quantify TEER in Ohms*cm^2 to enable comparison among different platforms.
Original languageEnglish
Title of host publication21st International Conference on Miniaturized Systems for Chemistry and Life Sciences
EditorsAbraham Lee, Don DeVoe
PublisherChemical and Biological Microstystems Society
Pages1114-1115
Number of pages2
ISBN (Electronic)978-0-692-94183-6
Publication statusPublished - 24 Oct 2017
Event21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS 2017 - Savannah International Trade & Convention Center, Savannah, United States
Duration: 22 Oct 201726 Oct 2017
Conference number: 21
https://www.microtas2017.org/

Publication series

NameInternational Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS)
Volume2017
ISSN (Print)1556-5904

Conference

Conference21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS 2017
Abbreviated titleMicroTAS
CountryUnited States
CitySavannah
Period22/10/1726/10/17
Internet address

Keywords

  • Transepithelial electrical resistance
  • Gut-on-a-chip
  • Impedance spectroscopy
  • Electrical simulations

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    van der Helm, M., Henry, O., Hamkins-Indik, T., Cronce, M., Leineweber, W., Odijk, M., ... Segerink, L. (2017). Electrical simulations and experimental results of 4-electrode AC transepithelial electrical resistance measurements in gut-on-a-chip. In A. Lee, & D. DeVoe (Eds.), 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 1114-1115). (International Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS); Vol. 2017). Chemical and Biological Microstystems Society.