Electrical simulations and experimental results of 4-electrode AC transepithelial electrical resistance measurements in gut-on-a-chip

Marinke van der Helm; Olivier Henry; Tiama Hamkins-Indik; Michael Cronce; William Leineweber; Mathieu  Odijk; Andries van der Meer; Jan Eijkel; Donald E. Ingber; Albert  van den Berg; Loes Segerink

Electrical simulations and experimental results of 4-electrode AC transepithelial electrical resistance measurements in gut-on-a-chip

Marinke van der Helm, Olivier Henry, Tiama Hamkins-Indik, Michael Cronce, William Leineweber, Mathieu Odijk, Andries van der Meer, Jan Eijkel, Donald E. Ingber, Albert van den Berg, Loes Segerink

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

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Abstract

Transepithelial electrical resistance (TEER) measurements are often used in organs-on-chips to monitor the barrier tightness of e.g. gut epithelium. Here, we present a chip with four integrated electrodes and a four-terminal alternating current (AC) measurement protocol to perform TEER measurements. The resulting impedance spectra are interpreted using electrical simulations, which include the chip with microfluidic channels, the four electrodes, the AC measurement protocol and the intestinal barrier cultured inside the device, which is modelled as a flat monolayer or as tissue with villi. Eventually, these simulations will be used to quantify TEER in Ohms*cm^2 to enable comparison among different platforms.

Original language	English
Title of host publication	21st International Conference on Miniaturized Systems for Chemistry and Life Sciences
Editors	Abraham Lee, Don DeVoe
Publisher	The Chemical and Biological Microsystems Society
Pages	1114-1115
Number of pages	2
ISBN (Electronic)	978-0-692-94183-6
Publication status	Published - 24 Oct 2017
Event	21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS 2017 - Savannah International Trade & Convention Center, Savannah, United States Duration: 22 Oct 2017 → 26 Oct 2017 Conference number: 21 https://www.microtas2017.org/

Publication series

Name	International Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS)
Volume	2017
ISSN (Print)	1556-5904

Conference

Conference	21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS 2017
Abbreviated title	MicroTAS
Country/Territory	United States
City	Savannah
Period	22/10/17 → 26/10/17
Internet address	https://www.microtas2017.org/

Keywords

Transepithelial electrical resistance
Gut-on-a-chip
Impedance spectroscopy
Electrical simulations

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van der Helm, M., Henry, O., Hamkins-Indik, T., Cronce, M., Leineweber, W., Odijk, M., van der Meer, A., Eijkel, J., Ingber, D. E., van den Berg, A., & Segerink, L. (2017). Electrical simulations and experimental results of 4-electrode AC transepithelial electrical resistance measurements in gut-on-a-chip. In A. Lee, & D. DeVoe (Eds.), 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 1114-1115). (International Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS); Vol. 2017). The Chemical and Biological Microsystems Society.

van der Helm, Marinke ; Henry, Olivier ; Hamkins-Indik, Tiama et al. / Electrical simulations and experimental results of 4-electrode AC transepithelial electrical resistance measurements in gut-on-a-chip. 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences. editor / Abraham Lee ; Don DeVoe. The Chemical and Biological Microsystems Society, 2017. pp. 1114-1115 (International Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS)).

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title = "Electrical simulations and experimental results of 4-electrode AC transepithelial electrical resistance measurements in gut-on-a-chip",

abstract = "Transepithelial electrical resistance (TEER) measurements are often used in organs-on-chips to monitor the barrier tightness of e.g. gut epithelium. Here, we present a chip with four integrated electrodes and a four-terminal alternating current (AC) measurement protocol to perform TEER measurements. The resulting impedance spectra are interpreted using electrical simulations, which include the chip with microfluidic channels, the four electrodes, the AC measurement protocol and the intestinal barrier cultured inside the device, which is modelled as a flat monolayer or as tissue with villi. Eventually, these simulations will be used to quantify TEER in Ohms*cm^2 to enable comparison among different platforms.",

keywords = "Transepithelial electrical resistance, Gut-on-a-chip, Impedance spectroscopy, Electrical simulations",

author = "{van der Helm}, Marinke and Olivier Henry and Tiama Hamkins-Indik and Michael Cronce and William Leineweber and Mathieu Odijk and {van der Meer}, Andries and Jan Eijkel and Ingber, {Donald E.} and {van den Berg}, Albert and Loes Segerink",

year = "2017",

month = oct,

day = "24",

language = "English",

series = "International Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS)",

publisher = "The Chemical and Biological Microsystems Society",

pages = "1114--1115",

editor = "Abraham Lee and Don DeVoe",

booktitle = "21st International Conference on Miniaturized Systems for Chemistry and Life Sciences",

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van der Helm, M, Henry, O, Hamkins-Indik, T, Cronce, M, Leineweber, W, Odijk, M , van der Meer, A , Eijkel, J, Ingber, DE, van den Berg, A & Segerink, L 2017, Electrical simulations and experimental results of 4-electrode AC transepithelial electrical resistance measurements in gut-on-a-chip. in A Lee & D DeVoe (eds), 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences. International Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS), vol. 2017, The Chemical and Biological Microsystems Society, pp. 1114-1115, 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS 2017, Savannah, Georgia, United States, 22/10/17.

Electrical simulations and experimental results of 4-electrode AC transepithelial electrical resistance measurements in gut-on-a-chip. / van der Helm, Marinke; Henry, Olivier; Hamkins-Indik, Tiama et al.
21st International Conference on Miniaturized Systems for Chemistry and Life Sciences. ed. / Abraham Lee; Don DeVoe. The Chemical and Biological Microsystems Society, 2017. p. 1114-1115 (International Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS); Vol. 2017).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

TY - GEN

T1 - Electrical simulations and experimental results of 4-electrode AC transepithelial electrical resistance measurements in gut-on-a-chip

AU - van der Helm, Marinke

AU - Henry, Olivier

AU - Hamkins-Indik, Tiama

AU - Cronce, Michael

AU - Leineweber, William

AU - Odijk, Mathieu

AU - van der Meer, Andries

AU - Eijkel, Jan

AU - Ingber, Donald E.

AU - van den Berg, Albert

AU - Segerink, Loes

N1 - Conference code: 21

PY - 2017/10/24

Y1 - 2017/10/24

N2 - Transepithelial electrical resistance (TEER) measurements are often used in organs-on-chips to monitor the barrier tightness of e.g. gut epithelium. Here, we present a chip with four integrated electrodes and a four-terminal alternating current (AC) measurement protocol to perform TEER measurements. The resulting impedance spectra are interpreted using electrical simulations, which include the chip with microfluidic channels, the four electrodes, the AC measurement protocol and the intestinal barrier cultured inside the device, which is modelled as a flat monolayer or as tissue with villi. Eventually, these simulations will be used to quantify TEER in Ohms*cm^2 to enable comparison among different platforms.

AB - Transepithelial electrical resistance (TEER) measurements are often used in organs-on-chips to monitor the barrier tightness of e.g. gut epithelium. Here, we present a chip with four integrated electrodes and a four-terminal alternating current (AC) measurement protocol to perform TEER measurements. The resulting impedance spectra are interpreted using electrical simulations, which include the chip with microfluidic channels, the four electrodes, the AC measurement protocol and the intestinal barrier cultured inside the device, which is modelled as a flat monolayer or as tissue with villi. Eventually, these simulations will be used to quantify TEER in Ohms*cm^2 to enable comparison among different platforms.

KW - Transepithelial electrical resistance

KW - Gut-on-a-chip

KW - Impedance spectroscopy

KW - Electrical simulations

M3 - Conference contribution

T3 - International Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS)

SP - 1114

EP - 1115

BT - 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences

A2 - Lee, Abraham

A2 - DeVoe, Don

PB - The Chemical and Biological Microsystems Society

T2 - 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS 2017

Y2 - 22 October 2017 through 26 October 2017

ER -

van der Helm M, Henry O, Hamkins-Indik T, Cronce M, Leineweber W, Odijk M et al. Electrical simulations and experimental results of 4-electrode AC transepithelial electrical resistance measurements in gut-on-a-chip. In Lee A, DeVoe D, editors, 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences. The Chemical and Biological Microsystems Society. 2017. p. 1114-1115. (International Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS)).

Electrical simulations and experimental results of 4-electrode AC transepithelial electrical resistance measurements in gut-on-a-chip

Abstract

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Keywords

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