Electrical simulations and experimental results of 4-electrode AC transepithelial electrical resistance measurements in gut-on-a-chip

Marieke Willemijn van der Helm, Olivier Henry, Tiama Hamkins-Indik, Michael Cronce, William Leineweber, Mathieu Odijk, Andries Dirk van der Meer, Jan C.T. Eijkel, Donald E. Ingber, Albert van den Berg, Loes Segerink

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Abstract

Transepithelial electrical resistance (TEER) measurements are often used in organs-on-chips to monitor the barrier tightness of e.g. gut epithelium. Here, we present a chip with four integrated electrodes and a four-terminal alternating current (AC) measurement protocol to perform TEER measurements. The resulting impedance spectra are interpreted using electrical simulations, which include the chip with microfluidic channels, the four electrodes, the AC measurement protocol and the intestinal barrier cultured inside the device, which is modelled as a flat monolayer or as tissue with villi. Eventually, these simulations will be used to quantify TEER in Ohms*cm^2 to enable comparison among different platforms.
Original languageEnglish
Title of host publication21st International Conference on Miniaturized Systems for Chemistry and Life Sciences
Place of PublicationSavannah
PublisherChemical and Biological Microstystems Society
Pages1114-1115
Number of pages2
ISBN (Electronic)978-0-692-94183-6
Publication statusPublished - 24 Oct 2017
Event21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS 2017 - Savannah International Trade & Convention Center, Savannah, United States
Duration: 22 Oct 201726 Oct 2017
Conference number: 21
https://www.microtas2017.org/

Conference

Conference21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS 2017
Abbreviated titleMicroTAS
CountryUnited States
CitySavannah
Period22/10/1726/10/17
Internet address

Fingerprint

electrical resistance
alternating current
chips
electrodes
simulation
tightness
epithelium
organs
platforms
impedance

Keywords

  • Transepithelial electrical resistance
  • gut-on-a-chip
  • impedance spectroscopy
  • electrical simulations

Cite this

van der Helm, M. W., Henry, O., Hamkins-Indik, T., Cronce, M., Leineweber, W., Odijk, M., ... Segerink, L. (2017). Electrical simulations and experimental results of 4-electrode AC transepithelial electrical resistance measurements in gut-on-a-chip. In 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 1114-1115). Savannah: Chemical and Biological Microstystems Society.
van der Helm, Marieke Willemijn ; Henry, Olivier ; Hamkins-Indik, Tiama ; Cronce, Michael ; Leineweber, William ; Odijk, Mathieu ; van der Meer, Andries Dirk ; Eijkel, Jan C.T. ; Ingber, Donald E. ; van den Berg, Albert ; Segerink, Loes. / Electrical simulations and experimental results of 4-electrode AC transepithelial electrical resistance measurements in gut-on-a-chip. 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences. Savannah : Chemical and Biological Microstystems Society, 2017. pp. 1114-1115
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abstract = "Transepithelial electrical resistance (TEER) measurements are often used in organs-on-chips to monitor the barrier tightness of e.g. gut epithelium. Here, we present a chip with four integrated electrodes and a four-terminal alternating current (AC) measurement protocol to perform TEER measurements. The resulting impedance spectra are interpreted using electrical simulations, which include the chip with microfluidic channels, the four electrodes, the AC measurement protocol and the intestinal barrier cultured inside the device, which is modelled as a flat monolayer or as tissue with villi. Eventually, these simulations will be used to quantify TEER in Ohms*cm^2 to enable comparison among different platforms.",
keywords = "Transepithelial electrical resistance, gut-on-a-chip, impedance spectroscopy, electrical simulations",
author = "{van der Helm}, {Marieke Willemijn} and Olivier Henry and Tiama Hamkins-Indik and Michael Cronce and William Leineweber and Mathieu Odijk and {van der Meer}, {Andries Dirk} and Eijkel, {Jan C.T.} and Ingber, {Donald E.} and {van den Berg}, Albert and Loes Segerink",
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van der Helm, MW, Henry, O, Hamkins-Indik, T, Cronce, M, Leineweber, W, Odijk, M, van der Meer, AD, Eijkel, JCT, Ingber, DE, van den Berg, A & Segerink, L 2017, Electrical simulations and experimental results of 4-electrode AC transepithelial electrical resistance measurements in gut-on-a-chip. in 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microstystems Society, Savannah, pp. 1114-1115, 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS 2017, Savannah, United States, 22/10/17.

Electrical simulations and experimental results of 4-electrode AC transepithelial electrical resistance measurements in gut-on-a-chip. / van der Helm, Marieke Willemijn; Henry, Olivier; Hamkins-Indik, Tiama; Cronce, Michael; Leineweber, William; Odijk, Mathieu ; van der Meer, Andries Dirk; Eijkel, Jan C.T.; Ingber, Donald E.; van den Berg, Albert ; Segerink, Loes.

21st International Conference on Miniaturized Systems for Chemistry and Life Sciences. Savannah : Chemical and Biological Microstystems Society, 2017. p. 1114-1115.

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

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T1 - Electrical simulations and experimental results of 4-electrode AC transepithelial electrical resistance measurements in gut-on-a-chip

AU - van der Helm, Marieke Willemijn

AU - Henry, Olivier

AU - Hamkins-Indik, Tiama

AU - Cronce, Michael

AU - Leineweber, William

AU - Odijk, Mathieu

AU - van der Meer, Andries Dirk

AU - Eijkel, Jan C.T.

AU - Ingber, Donald E.

AU - van den Berg, Albert

AU - Segerink, Loes

PY - 2017/10/24

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N2 - Transepithelial electrical resistance (TEER) measurements are often used in organs-on-chips to monitor the barrier tightness of e.g. gut epithelium. Here, we present a chip with four integrated electrodes and a four-terminal alternating current (AC) measurement protocol to perform TEER measurements. The resulting impedance spectra are interpreted using electrical simulations, which include the chip with microfluidic channels, the four electrodes, the AC measurement protocol and the intestinal barrier cultured inside the device, which is modelled as a flat monolayer or as tissue with villi. Eventually, these simulations will be used to quantify TEER in Ohms*cm^2 to enable comparison among different platforms.

AB - Transepithelial electrical resistance (TEER) measurements are often used in organs-on-chips to monitor the barrier tightness of e.g. gut epithelium. Here, we present a chip with four integrated electrodes and a four-terminal alternating current (AC) measurement protocol to perform TEER measurements. The resulting impedance spectra are interpreted using electrical simulations, which include the chip with microfluidic channels, the four electrodes, the AC measurement protocol and the intestinal barrier cultured inside the device, which is modelled as a flat monolayer or as tissue with villi. Eventually, these simulations will be used to quantify TEER in Ohms*cm^2 to enable comparison among different platforms.

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KW - impedance spectroscopy

KW - electrical simulations

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BT - 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences

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van der Helm MW, Henry O, Hamkins-Indik T, Cronce M, Leineweber W, Odijk M et al. Electrical simulations and experimental results of 4-electrode AC transepithelial electrical resistance measurements in gut-on-a-chip. In 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences. Savannah: Chemical and Biological Microstystems Society. 2017. p. 1114-1115