TY - JOUR
T1 - Electrically Stimulated Adipose Stem Cells on Polypyrrole-Coated Scaffolds for Smooth Muscle Tissue Engineering
AU - Björninen, Miina
AU - Gilmore, Kerry
AU - Pelto, Jani
AU - Seppänen-Kaijansinkko, Riitta
AU - Kellomäki, Minna
AU - Miettinen, Susanna
AU - Wallace, Gordon
AU - Grijpma, Dirk
AU - Haimi, Suvi
N1 - Funding Information:
The authors would like to thank Salvador Jimenes for assistance with the experiments, Thomas van Berkel for fabrication of the PTMC scaffolds as well as assisting with the experiments, and Elina Talvitie for fabricating the PPy-coatings. Authors also owe their gratitude to Sue Lyn Ku, Lydia Bolhuis-Versteeg, Miia Juntunen, Anna-Maija Honkala, and Sari Kalliokoski for technical assistance in cell culture. This work was carried out with the financial support of the Finnish Funding Agency for Technology and Innovation (TEKES); the Academy of Finland, the Paulo Foundation, the Science Centre of Tampere City, the Finnish Dental Society Apollonia and the ARC Centre of Excellence in Electromaterials Science at the University of Wollongong.
Publisher Copyright:
© 2016, Biomedical Engineering Society.
PY - 2017/4/1
Y1 - 2017/4/1
N2 - We investigated the use of polypyrrole (PPy)-coated polymer scaffolds and electrical stimulation (ES) to differentiate adipose stem cells (ASCs) towards smooth muscle cells (SMCs). Since tissue engineering lacks robust and reusable 3D ES devices we developed a device that can deliver ES in a reliable, repeatable, and cost-efficient way in a 3D environment. Long pulse (1 ms) or short pulse (0.25 ms) biphasic electric current at a frequency of 10 Hz was applied to ASCs to study the effects of ES on ASC viability and differentiation towards SMCs on the PPy-coated scaffolds. PPy-coated scaffolds promoted proliferation and induced stronger calponin, myosin heavy chain (MHC) and smooth muscle actin (SMA) expression in ASCs compared to uncoated scaffolds. ES with 1 ms pulse width increased the number of viable cells by day 7 compared to controls and remained at similar levels to controls by day 14, whereas shorter pulses significantly decreased viability compared to the other groups. Both ES protocols supported smooth muscle expression markers. Our results indicate that electrical stimulation on PPy-coated scaffolds applied through the novel 3D ES device is a valid approach for vascular smooth muscle tissue engineering.
AB - We investigated the use of polypyrrole (PPy)-coated polymer scaffolds and electrical stimulation (ES) to differentiate adipose stem cells (ASCs) towards smooth muscle cells (SMCs). Since tissue engineering lacks robust and reusable 3D ES devices we developed a device that can deliver ES in a reliable, repeatable, and cost-efficient way in a 3D environment. Long pulse (1 ms) or short pulse (0.25 ms) biphasic electric current at a frequency of 10 Hz was applied to ASCs to study the effects of ES on ASC viability and differentiation towards SMCs on the PPy-coated scaffolds. PPy-coated scaffolds promoted proliferation and induced stronger calponin, myosin heavy chain (MHC) and smooth muscle actin (SMA) expression in ASCs compared to uncoated scaffolds. ES with 1 ms pulse width increased the number of viable cells by day 7 compared to controls and remained at similar levels to controls by day 14, whereas shorter pulses significantly decreased viability compared to the other groups. Both ES protocols supported smooth muscle expression markers. Our results indicate that electrical stimulation on PPy-coated scaffolds applied through the novel 3D ES device is a valid approach for vascular smooth muscle tissue engineering.
KW - Conductive polymers
KW - Mesenchymal stem cells
KW - Physical stimulation
KW - Poly (trimethylene carbonate)
KW - Vascular tissue engineering
KW - 22/1 OA procedure
UR - http://www.scopus.com/inward/record.url?scp=84995520523&partnerID=8YFLogxK
U2 - 10.1007/s10439-016-1755-7
DO - 10.1007/s10439-016-1755-7
M3 - Article
C2 - 27844175
SN - 0090-6964
VL - 45
SP - 1015
EP - 1026
JO - Annals of biomedical engineering
JF - Annals of biomedical engineering
IS - 4
ER -