Electrochemical detection of enzyme kinetics using a nanofluidic thin layer cell device

E.D. Goluch, N. Wongrajit, P.S. Singh, A.W.J.W. Tepper, H.A. Heering, G.W. Canters, S.G. Lemay

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    Abstract

    Amperometric detection is an attractive detection scheme for mico- and nanofluidic systems as it directly yields an electrical signal. However, this scheme is not often utilized because of its limited sensitivity. We overcome this limitation by employing a nanofluidic device consisting of a solution-filled cavity bounded by two closely spaced parallel electrodes that can amplify the current by repeatedly reducing and oxidizing electroactive species. Here we demonstrate the utility of such a device to measure enzyme kinetics in real time. We compare the conversion of p-cresol into methylquinone by tyrosinase using our nanofluidic device against a standard UV-Vis spectroscopic protocol.
    Original languageEnglish
    Title of host publication14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
    EditorsSabeth Verpoorte, Helen Andersson-Svahn, Jenny Emnéus, Nicole Pamme
    PublisherThe Chemical and Biological Microsystems Society
    Pages482-484
    ISBN (Print)978-0-9798064-3-8
    Publication statusPublished - 3 Oct 2010
    Event14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, µTAS 2010 - Groningen, Netherlands
    Duration: 3 Oct 20107 Oct 2010
    Conference number: 14

    Publication series

    NameInternational Conference on Miniaterized Systems for Chemistry and Life Sciences : [proceedings]
    PublisherChemical and Biological Microsystems Society
    Volume2010
    ISSN (Print)1556-5904

    Conference

    Conference14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, µTAS 2010
    Abbreviated titleMicroTAS
    CountryNetherlands
    CityGroningen
    Period3/10/107/10/10

    Keywords

    • Electrochemistry
    • Redox Cycling
    • Enzyme Kinetics
    • Nanofluidics

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