Electrochemical detection of enzyme kinetics using a nanofluidic thin layer cell device

E.D. Goluch; N. Wongrajit; P.S. Singh; A.W.J.W. Tepper; H.A. Heering; G.W. Canters; S.G. Lemay

Electrochemical detection of enzyme kinetics using a nanofluidic thin layer cell device

E.D. Goluch
, N. Wongrajit
, P.S. Singh
, A.W.J.W. Tepper
, H.A. Heering
, G.W. Canters
, S.G. Lemay

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

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Abstract

Amperometric detection is an attractive detection scheme for mico- and nanofluidic systems as it directly yields an electrical signal. However, this scheme is not often utilized because of its limited sensitivity. We overcome this limitation by employing a nanofluidic device consisting of a solution-filled cavity bounded by two closely spaced parallel electrodes that can amplify the current by repeatedly reducing and oxidizing electroactive species. Here we demonstrate the utility of such a device to measure enzyme kinetics in real time. We compare the conversion of p-cresol into methylquinone by tyrosinase using our nanofluidic device against a standard UV-Vis spectroscopic protocol.

Original language	English
Title of host publication	14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Editors	Sabeth Verpoorte, Helen Andersson-Svahn, Jenny Emnéus, Nicole Pamme
Publisher	The Chemical and Biological Microsystems Society
Pages	482-484
ISBN (Print)	978-0-9798064-3-8
Publication status	Published - 3 Oct 2010
Event	14th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2010 - Groningen, Netherlands Duration: 3 Oct 2010 → 7 Oct 2010 Conference number: 14

Publication series

Name	International Conference on Miniaterized Systems for Chemistry and Life Sciences : [proceedings]
Publisher	Chemical and Biological Microsystems Society
Volume	2010
ISSN (Print)	1556-5904

Conference

Conference	14th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2010
Abbreviated title	MicroTAS 2010
Country/Territory	Netherlands
City	Groningen
Period	3/10/10 → 7/10/10

Keywords

Electrochemistry
Redox Cycling
Enzyme Kinetics
Nanofluidics

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Goluch, E. D., Wongrajit, N., Singh, P. S., Tepper, A. W. J. W., Heering, H. A., Canters, G. W., & Lemay, S. G. (2010). Electrochemical detection of enzyme kinetics using a nanofluidic thin layer cell device. In S. Verpoorte, H. Andersson-Svahn, J. Emnéus, & N. Pamme (Eds.), 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 (pp. 482-484). (International Conference on Miniaterized Systems for Chemistry and Life Sciences : [proceedings]; Vol. 2010). The Chemical and Biological Microsystems Society.

Goluch, E.D. ; Wongrajit, N. ; Singh, P.S. et al. / Electrochemical detection of enzyme kinetics using a nanofluidic thin layer cell device. 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010. editor / Sabeth Verpoorte ; Helen Andersson-Svahn ; Jenny Emnéus ; Nicole Pamme. The Chemical and Biological Microsystems Society, 2010. pp. 482-484 (International Conference on Miniaterized Systems for Chemistry and Life Sciences : [proceedings]).

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title = "Electrochemical detection of enzyme kinetics using a nanofluidic thin layer cell device",

abstract = "Amperometric detection is an attractive detection scheme for mico- and nanofluidic systems as it directly yields an electrical signal. However, this scheme is not often utilized because of its limited sensitivity. We overcome this limitation by employing a nanofluidic device consisting of a solution-filled cavity bounded by two closely spaced parallel electrodes that can amplify the current by repeatedly reducing and oxidizing electroactive species. Here we demonstrate the utility of such a device to measure enzyme kinetics in real time. We compare the conversion of p-cresol into methylquinone by tyrosinase using our nanofluidic device against a standard UV-Vis spectroscopic protocol.",

keywords = "Electrochemistry, Redox Cycling, Enzyme Kinetics, Nanofluidics",

author = "E.D. Goluch and N. Wongrajit and P.S. Singh and A.W.J.W. Tepper and H.A. Heering and G.W. Canters and S.G. Lemay",

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editor = "Sabeth Verpoorte and Helen Andersson-Svahn and Jenny Emn{\'e}us and Nicole Pamme",

booktitle = "14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010",

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Goluch, ED, Wongrajit, N, Singh, PS, Tepper, AWJW, Heering, HA, Canters, GW & Lemay, SG 2010, Electrochemical detection of enzyme kinetics using a nanofluidic thin layer cell device. in S Verpoorte, H Andersson-Svahn, J Emnéus & N Pamme (eds), 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010. International Conference on Miniaterized Systems for Chemistry and Life Sciences : [proceedings], vol. 2010, The Chemical and Biological Microsystems Society, pp. 482-484, 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2010, Groningen, Netherlands, 3/10/10.

Electrochemical detection of enzyme kinetics using a nanofluidic thin layer cell device. / Goluch, E.D.; Wongrajit, N.; Singh, P.S. et al.
14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010. ed. / Sabeth Verpoorte; Helen Andersson-Svahn; Jenny Emnéus; Nicole Pamme. The Chemical and Biological Microsystems Society, 2010. p. 482-484 (International Conference on Miniaterized Systems for Chemistry and Life Sciences : [proceedings]; Vol. 2010).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

TY - GEN

T1 - Electrochemical detection of enzyme kinetics using a nanofluidic thin layer cell device

AU - Goluch, E.D.

AU - Wongrajit, N.

AU - Singh, P.S.

AU - Tepper, A.W.J.W.

AU - Heering, H.A.

AU - Canters, G.W.

AU - Lemay, S.G.

N1 - Conference code: 14

PY - 2010/10/3

Y1 - 2010/10/3

N2 - Amperometric detection is an attractive detection scheme for mico- and nanofluidic systems as it directly yields an electrical signal. However, this scheme is not often utilized because of its limited sensitivity. We overcome this limitation by employing a nanofluidic device consisting of a solution-filled cavity bounded by two closely spaced parallel electrodes that can amplify the current by repeatedly reducing and oxidizing electroactive species. Here we demonstrate the utility of such a device to measure enzyme kinetics in real time. We compare the conversion of p-cresol into methylquinone by tyrosinase using our nanofluidic device against a standard UV-Vis spectroscopic protocol.

AB - Amperometric detection is an attractive detection scheme for mico- and nanofluidic systems as it directly yields an electrical signal. However, this scheme is not often utilized because of its limited sensitivity. We overcome this limitation by employing a nanofluidic device consisting of a solution-filled cavity bounded by two closely spaced parallel electrodes that can amplify the current by repeatedly reducing and oxidizing electroactive species. Here we demonstrate the utility of such a device to measure enzyme kinetics in real time. We compare the conversion of p-cresol into methylquinone by tyrosinase using our nanofluidic device against a standard UV-Vis spectroscopic protocol.

KW - Electrochemistry

KW - Redox Cycling

KW - Enzyme Kinetics

KW - Nanofluidics

M3 - Conference contribution

SN - 978-0-9798064-3-8

T3 - International Conference on Miniaterized Systems for Chemistry and Life Sciences : [proceedings]

SP - 482

EP - 484

BT - 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010

A2 - Verpoorte, Sabeth

A2 - Andersson-Svahn, Helen

A2 - Emnéus, Jenny

A2 - Pamme, Nicole

PB - The Chemical and Biological Microsystems Society

T2 - 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2010

Y2 - 3 October 2010 through 7 October 2010

ER -

Goluch ED, Wongrajit N, Singh PS, Tepper AWJW, Heering HA, Canters GW et al. Electrochemical detection of enzyme kinetics using a nanofluidic thin layer cell device. In Verpoorte S, Andersson-Svahn H, Emnéus J, Pamme N, editors, 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010. The Chemical and Biological Microsystems Society. 2010. p. 482-484. (International Conference on Miniaterized Systems for Chemistry and Life Sciences : [proceedings]).

Electrochemical detection of enzyme kinetics using a nanofluidic thin layer cell device

Abstract

Publication series

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Keywords

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