Electrochemical protein cleavage in a microfluidic cell for proteomics studies

Floris T.G. van den Brink, Tao Zhang, Liwei Ma, Mathieu Odijk, Wouter Olthuis, Hjalmar P. Permentier, Rainer P.H. Bischoff, Albert van den Berg

    Research output: Contribution to journalArticleAcademicpeer-review

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    Abstract

    Electrochemical protein digestion prior to mass spectrometric analysis is a purely instrumental approach to protein identification, offering reduced consumption of chemicals and shorter analysis times compared to the use of enzymes and chemical cleavage agents. Here we demonstrate the possibilities of site-specific peptide bond cleavage and disulphide bond reduction in a microfluidic electrochemical cell. The use of microfluidics in this context is beneficial for increased electrochemical cleavage yields, small sample volumes and the possibility of rapid on-line analysis, thereby providing a versatile tool for routine proteomics studies.
    Original languageEnglish
    Pages (from-to)62-64
    JournalProcedia technology
    Volume27
    DOIs
    Publication statusPublished - 2017

    Keywords

    • Peptide bond cleavage
    • Disulphide bond reduction
    • Electrochemical protein digestion
    • Microfluidic electrochemical cell
    • Electrochemistry/Mass spectrometry
    • Proteomics

    Cite this

    van den Brink, Floris T.G. ; Zhang, Tao ; Ma, Liwei ; Odijk, Mathieu ; Olthuis, Wouter ; Permentier, Hjalmar P. ; Bischoff, Rainer P.H. ; van den Berg, Albert . / Electrochemical protein cleavage in a microfluidic cell for proteomics studies. In: Procedia technology. 2017 ; Vol. 27. pp. 62-64.
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    abstract = "Electrochemical protein digestion prior to mass spectrometric analysis is a purely instrumental approach to protein identification, offering reduced consumption of chemicals and shorter analysis times compared to the use of enzymes and chemical cleavage agents. Here we demonstrate the possibilities of site-specific peptide bond cleavage and disulphide bond reduction in a microfluidic electrochemical cell. The use of microfluidics in this context is beneficial for increased electrochemical cleavage yields, small sample volumes and the possibility of rapid on-line analysis, thereby providing a versatile tool for routine proteomics studies.",
    keywords = "Peptide bond cleavage, Disulphide bond reduction, Electrochemical protein digestion, Microfluidic electrochemical cell, Electrochemistry/Mass spectrometry, Proteomics",
    author = "{van den Brink}, {Floris T.G.} and Tao Zhang and Liwei Ma and Mathieu Odijk and Wouter Olthuis and Permentier, {Hjalmar P.} and Bischoff, {Rainer P.H.} and {van den Berg}, Albert",
    year = "2017",
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    Electrochemical protein cleavage in a microfluidic cell for proteomics studies. / van den Brink, Floris T.G.; Zhang, Tao; Ma, Liwei; Odijk, Mathieu ; Olthuis, Wouter; Permentier, Hjalmar P.; Bischoff, Rainer P.H.; van den Berg, Albert .

    In: Procedia technology, Vol. 27, 2017, p. 62-64.

    Research output: Contribution to journalArticleAcademicpeer-review

    TY - JOUR

    T1 - Electrochemical protein cleavage in a microfluidic cell for proteomics studies

    AU - van den Brink, Floris T.G.

    AU - Zhang, Tao

    AU - Ma, Liwei

    AU - Odijk, Mathieu

    AU - Olthuis, Wouter

    AU - Permentier, Hjalmar P.

    AU - Bischoff, Rainer P.H.

    AU - van den Berg, Albert

    PY - 2017

    Y1 - 2017

    N2 - Electrochemical protein digestion prior to mass spectrometric analysis is a purely instrumental approach to protein identification, offering reduced consumption of chemicals and shorter analysis times compared to the use of enzymes and chemical cleavage agents. Here we demonstrate the possibilities of site-specific peptide bond cleavage and disulphide bond reduction in a microfluidic electrochemical cell. The use of microfluidics in this context is beneficial for increased electrochemical cleavage yields, small sample volumes and the possibility of rapid on-line analysis, thereby providing a versatile tool for routine proteomics studies.

    AB - Electrochemical protein digestion prior to mass spectrometric analysis is a purely instrumental approach to protein identification, offering reduced consumption of chemicals and shorter analysis times compared to the use of enzymes and chemical cleavage agents. Here we demonstrate the possibilities of site-specific peptide bond cleavage and disulphide bond reduction in a microfluidic electrochemical cell. The use of microfluidics in this context is beneficial for increased electrochemical cleavage yields, small sample volumes and the possibility of rapid on-line analysis, thereby providing a versatile tool for routine proteomics studies.

    KW - Peptide bond cleavage

    KW - Disulphide bond reduction

    KW - Electrochemical protein digestion

    KW - Microfluidic electrochemical cell

    KW - Electrochemistry/Mass spectrometry

    KW - Proteomics

    U2 - 10.1016/j.protcy.2017.04.029

    DO - 10.1016/j.protcy.2017.04.029

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    EP - 64

    JO - Procedia technology

    JF - Procedia technology

    SN - 2212-0173

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