TY - JOUR
T1 - Electrochemical protein cleavage in a microfluidic cell for proteomics studies
AU - van den Brink, Floris T.G.
AU - Zhang, Tao
AU - Ma, Liwei
AU - Odijk, Mathieu
AU - Olthuis, Wouter
AU - Permentier, Hjalmar P.
AU - Bischoff, Rainer P.H.
AU - van den Berg, Albert
PY - 2017
Y1 - 2017
N2 - Electrochemical protein digestion prior to mass spectrometric analysis is a purely instrumental approach to protein identification, offering reduced consumption of chemicals and shorter analysis times compared to the use of enzymes and chemical cleavage agents. Here we demonstrate the possibilities of site-specific peptide bond cleavage and disulphide bond reduction in a microfluidic electrochemical cell. The use of microfluidics in this context is beneficial for increased electrochemical cleavage yields, small sample volumes and the possibility of rapid on-line analysis, thereby providing a versatile tool for routine proteomics studies.
AB - Electrochemical protein digestion prior to mass spectrometric analysis is a purely instrumental approach to protein identification, offering reduced consumption of chemicals and shorter analysis times compared to the use of enzymes and chemical cleavage agents. Here we demonstrate the possibilities of site-specific peptide bond cleavage and disulphide bond reduction in a microfluidic electrochemical cell. The use of microfluidics in this context is beneficial for increased electrochemical cleavage yields, small sample volumes and the possibility of rapid on-line analysis, thereby providing a versatile tool for routine proteomics studies.
KW - Peptide bond cleavage
KW - Disulphide bond reduction
KW - Electrochemical protein digestion
KW - Microfluidic electrochemical cell
KW - Electrochemistry/Mass spectrometry
KW - Proteomics
U2 - 10.1016/j.protcy.2017.04.029
DO - 10.1016/j.protcy.2017.04.029
M3 - Article
SN - 2212-0173
VL - 27
SP - 62
EP - 64
JO - Procedia technology
JF - Procedia technology
ER -