Electrochemical protein cleavage in a microfluidic cell for proteomics studies

Floris T.G. van den Brink, Tao Zhang, Liwei Ma, Mathieu Odijk, Wouter Olthuis, Hjalmar P. Permentier, Rainer P.H. Bischoff, Albert van den Berg

    Research output: Contribution to journalArticleAcademicpeer-review

    71 Downloads (Pure)

    Abstract

    Electrochemical protein digestion prior to mass spectrometric analysis is a purely instrumental approach to protein identification, offering reduced consumption of chemicals and shorter analysis times compared to the use of enzymes and chemical cleavage agents. Here we demonstrate the possibilities of site-specific peptide bond cleavage and disulphide bond reduction in a microfluidic electrochemical cell. The use of microfluidics in this context is beneficial for increased electrochemical cleavage yields, small sample volumes and the possibility of rapid on-line analysis, thereby providing a versatile tool for routine proteomics studies.
    Original languageEnglish
    Pages (from-to)62-64
    JournalProcedia technology
    Volume27
    DOIs
    Publication statusPublished - 2017

    Keywords

    • Peptide bond cleavage
    • Disulphide bond reduction
    • Electrochemical protein digestion
    • Microfluidic electrochemical cell
    • Electrochemistry/Mass spectrometry
    • Proteomics

    Cite this