Electrochemical protein cleavage in a microfluidic cell with integrated boron doped diamond electrodes

Floris Teunis Gerardus van den Brink, Tao Zhang, Liwei Ma, Johan G. Bomer, Mathieu Odijk, Wouter Olthuis, Hjalmar P. Permentier, Rainer Bischoff, Albert van den Berg

    Research output: Contribution to journalArticleAcademicpeer-review

    7 Citations (Scopus)

    Abstract

    Specific electrochemical cleavage of peptide bonds at the C-terminal side of tyrosine and tryptophan generates peptides amenable to liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis for protein identification. To this end we developed a microfluidic electrochemical cell of 160 nL volume that combines a cell geometry optimized for a high electrochemical conversion efficiency (>95%) with an integrated boron doped diamond (BDD) working electrode offering a wide potential window in aqueous solution and reduced adsorption of peptides and proteins. Efficient cleavage of the proteins bovine insulin and chicken egg white lysozyme was observed at 4 out of 4 and 7 out of 9 of the predicted cleavage sites, respectively. Chicken egg white lysozyme was identified based on 5 electrochemically generated peptides using a proteomics database searching algorithm. These results show that electrochemical peptide bond cleavage in a microfluidic cell is a novel, fully instrumental approach toward protein analysis and eventually proteomics studies in conjunction with mass spectrometry
    Original languageEnglish
    Pages (from-to)9190-9198
    Number of pages9
    JournalAnalytical chemistry
    Volume88
    Issue number18
    DOIs
    Publication statusPublished - 20 Sep 2016

    Fingerprint

    Diamond
    Boron
    Microfluidics
    Peptides
    Electrodes
    Muramidase
    Proteins
    Mass spectrometry
    Electrochemical cells
    Liquid chromatography
    Tryptophan
    Conversion efficiency
    Tyrosine
    Insulin
    Adsorption
    Geometry

    Keywords

    • EWI-27326
    • IR-101825
    • METIS-318560

    Cite this

    van den Brink, Floris Teunis Gerardus ; Zhang, Tao ; Ma, Liwei ; Bomer, Johan G. ; Odijk, Mathieu ; Olthuis, Wouter ; Permentier, Hjalmar P. ; Bischoff, Rainer ; van den Berg, Albert. / Electrochemical protein cleavage in a microfluidic cell with integrated boron doped diamond electrodes. In: Analytical chemistry. 2016 ; Vol. 88, No. 18. pp. 9190-9198.
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    abstract = "Specific electrochemical cleavage of peptide bonds at the C-terminal side of tyrosine and tryptophan generates peptides amenable to liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis for protein identification. To this end we developed a microfluidic electrochemical cell of 160 nL volume that combines a cell geometry optimized for a high electrochemical conversion efficiency (>95{\%}) with an integrated boron doped diamond (BDD) working electrode offering a wide potential window in aqueous solution and reduced adsorption of peptides and proteins. Efficient cleavage of the proteins bovine insulin and chicken egg white lysozyme was observed at 4 out of 4 and 7 out of 9 of the predicted cleavage sites, respectively. Chicken egg white lysozyme was identified based on 5 electrochemically generated peptides using a proteomics database searching algorithm. These results show that electrochemical peptide bond cleavage in a microfluidic cell is a novel, fully instrumental approach toward protein analysis and eventually proteomics studies in conjunction with mass spectrometry",
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    author = "{van den Brink}, {Floris Teunis Gerardus} and Tao Zhang and Liwei Ma and Bomer, {Johan G.} and Mathieu Odijk and Wouter Olthuis and Permentier, {Hjalmar P.} and Rainer Bischoff and {van den Berg}, Albert",
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    Electrochemical protein cleavage in a microfluidic cell with integrated boron doped diamond electrodes. / van den Brink, Floris Teunis Gerardus; Zhang, Tao; Ma, Liwei; Bomer, Johan G.; Odijk, Mathieu; Olthuis, Wouter; Permentier, Hjalmar P.; Bischoff, Rainer; van den Berg, Albert.

    In: Analytical chemistry, Vol. 88, No. 18, 20.09.2016, p. 9190-9198.

    Research output: Contribution to journalArticleAcademicpeer-review

    TY - JOUR

    T1 - Electrochemical protein cleavage in a microfluidic cell with integrated boron doped diamond electrodes

    AU - van den Brink, Floris Teunis Gerardus

    AU - Zhang, Tao

    AU - Ma, Liwei

    AU - Bomer, Johan G.

    AU - Odijk, Mathieu

    AU - Olthuis, Wouter

    AU - Permentier, Hjalmar P.

    AU - Bischoff, Rainer

    AU - van den Berg, Albert

    N1 - eemcs-eprint-27326

    PY - 2016/9/20

    Y1 - 2016/9/20

    N2 - Specific electrochemical cleavage of peptide bonds at the C-terminal side of tyrosine and tryptophan generates peptides amenable to liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis for protein identification. To this end we developed a microfluidic electrochemical cell of 160 nL volume that combines a cell geometry optimized for a high electrochemical conversion efficiency (>95%) with an integrated boron doped diamond (BDD) working electrode offering a wide potential window in aqueous solution and reduced adsorption of peptides and proteins. Efficient cleavage of the proteins bovine insulin and chicken egg white lysozyme was observed at 4 out of 4 and 7 out of 9 of the predicted cleavage sites, respectively. Chicken egg white lysozyme was identified based on 5 electrochemically generated peptides using a proteomics database searching algorithm. These results show that electrochemical peptide bond cleavage in a microfluidic cell is a novel, fully instrumental approach toward protein analysis and eventually proteomics studies in conjunction with mass spectrometry

    AB - Specific electrochemical cleavage of peptide bonds at the C-terminal side of tyrosine and tryptophan generates peptides amenable to liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis for protein identification. To this end we developed a microfluidic electrochemical cell of 160 nL volume that combines a cell geometry optimized for a high electrochemical conversion efficiency (>95%) with an integrated boron doped diamond (BDD) working electrode offering a wide potential window in aqueous solution and reduced adsorption of peptides and proteins. Efficient cleavage of the proteins bovine insulin and chicken egg white lysozyme was observed at 4 out of 4 and 7 out of 9 of the predicted cleavage sites, respectively. Chicken egg white lysozyme was identified based on 5 electrochemically generated peptides using a proteomics database searching algorithm. These results show that electrochemical peptide bond cleavage in a microfluidic cell is a novel, fully instrumental approach toward protein analysis and eventually proteomics studies in conjunction with mass spectrometry

    KW - EWI-27326

    KW - IR-101825

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    DO - 10.1021/acs.analchem.6b02413

    M3 - Article

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    JO - Analytical chemistry

    JF - Analytical chemistry

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    ER -