Electrochemical Single-Molecule Detection in Aqueous Solution Using Self-Aligned Nanogap Transducers

Shuo Kang, Albert F. Nieuwenhuis, Klaus Mathwig, Dileep Mampallil Augustine, Serge Joseph Guy Lemay

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53 Citations (Scopus)


Electrochemical detection of individual molecular tags in nanochannels may enable cost-effective, massively parallel analysis and diagnostics platforms. Here we demonstrate single-molecule detection of prototypical analytes in aqueous solution based on redox cycling in 40 nm nanogap transducers. These nanofluidic devices are fabricated using standard microfabrication techniques combined with a self-aligned approach that minimizes gap size and dead volume. We demonstrate the detection of three common redox mediators at physiological salt concentrations.
Original languageEnglish
Pages (from-to)10931-10937
JournalACS nano
Issue number12
Publication statusPublished - 26 Nov 2013



  • METIS-309979
  • IR-95449

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