Electrode‑assisted trapping and of droplets on hydrophilic in a hydrophobic microchannel

Research output: Contribution to journalArticleAcademicpeer-review

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Abstract

In two-phase flow microfluidics, there is an increasing interest in technologies which enable the encapsulation of biological cells into aqueous drops and the subsequent study of their molecular (excretion or lysis) products. One not yet available but very promising analysis method is the use of biospecific surface patches embedded in the wall of microfluidic channels. In this paper, we tackle some technological challenges encountered in the development of such applications. In the detection protocol, each drop must be enabled to wet the designated patch, be held in contact long enough for biomolecular detection and subsequently be released. This is engineered via a combination of well-defined chemical sites in the walls of the flow channel and insulated microelectrodes. The tunability of the local electric field allows to modify the competition between chemical (pinning) forces which tend to immobilize the drop and hydrodynamic forces which oppose this process. We developed a prototype microfluidic device which offers this functionality. A channel structure is sandwiched between an actuation surface with electrowetting (EW) electrodes on one side and a detector surface with a hydrophilic patch amidst a hydrophobic environment on the other. Two pairs of carefully aligned EW electrodes are used: one for drop adherence and another one for the subsequent release. We demonstrate these operations and discuss the required voltage signals in terms of the forces on the drop. Finally, we discuss possible steps for further improvement in the device.
Original languageEnglish
Pages (from-to)2-12
JournalMicrofluidics and nanofluidics
Volume123
DOIs
Publication statusPublished - 2016

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microchannels
Microchannels
trapping
Microfluidics
excretion
Electrodes
electrodes
microfluidic devices
Microelectrodes
channel flow
two phase flow
Channel flow
actuation
Encapsulation
Two phase flow
Hydrodynamics
hydrodynamics
prototypes
Electric fields
Detectors

Keywords

  • IR-102480
  • METIS-319522

Cite this

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title = "Electrode‑assisted trapping and of droplets on hydrophilic in a hydrophobic microchannel",
abstract = "In two-phase flow microfluidics, there is an increasing interest in technologies which enable the encapsulation of biological cells into aqueous drops and the subsequent study of their molecular (excretion or lysis) products. One not yet available but very promising analysis method is the use of biospecific surface patches embedded in the wall of microfluidic channels. In this paper, we tackle some technological challenges encountered in the development of such applications. In the detection protocol, each drop must be enabled to wet the designated patch, be held in contact long enough for biomolecular detection and subsequently be released. This is engineered via a combination of well-defined chemical sites in the walls of the flow channel and insulated microelectrodes. The tunability of the local electric field allows to modify the competition between chemical (pinning) forces which tend to immobilize the drop and hydrodynamic forces which oppose this process. We developed a prototype microfluidic device which offers this functionality. A channel structure is sandwiched between an actuation surface with electrowetting (EW) electrodes on one side and a detector surface with a hydrophilic patch amidst a hydrophobic environment on the other. Two pairs of carefully aligned EW electrodes are used: one for drop adherence and another one for the subsequent release. We demonstrate these operations and discuss the required voltage signals in terms of the forces on the drop. Finally, we discuss possible steps for further improvement in the device.",
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author = "Arjen Pit and S. Bonestroo and Dani{\"e}l Wijnperle and Duits, {Michael H.G.} and Mugele, {Friedrich Gunther}",
year = "2016",
doi = "10.1007/s10404-016-1789-z",
language = "English",
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pages = "2--12",
journal = "Microfluidics and nanofluidics",
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}

Electrode‑assisted trapping and of droplets on hydrophilic in a hydrophobic microchannel. / Pit, Arjen; Bonestroo, S.; Wijnperle, Daniël; Duits, Michael H.G.; Mugele, Friedrich Gunther.

In: Microfluidics and nanofluidics, Vol. 123, 2016, p. 2-12.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Electrode‑assisted trapping and of droplets on hydrophilic in a hydrophobic microchannel

AU - Pit, Arjen

AU - Bonestroo, S.

AU - Wijnperle, Daniël

AU - Duits, Michael H.G.

AU - Mugele, Friedrich Gunther

PY - 2016

Y1 - 2016

N2 - In two-phase flow microfluidics, there is an increasing interest in technologies which enable the encapsulation of biological cells into aqueous drops and the subsequent study of their molecular (excretion or lysis) products. One not yet available but very promising analysis method is the use of biospecific surface patches embedded in the wall of microfluidic channels. In this paper, we tackle some technological challenges encountered in the development of such applications. In the detection protocol, each drop must be enabled to wet the designated patch, be held in contact long enough for biomolecular detection and subsequently be released. This is engineered via a combination of well-defined chemical sites in the walls of the flow channel and insulated microelectrodes. The tunability of the local electric field allows to modify the competition between chemical (pinning) forces which tend to immobilize the drop and hydrodynamic forces which oppose this process. We developed a prototype microfluidic device which offers this functionality. A channel structure is sandwiched between an actuation surface with electrowetting (EW) electrodes on one side and a detector surface with a hydrophilic patch amidst a hydrophobic environment on the other. Two pairs of carefully aligned EW electrodes are used: one for drop adherence and another one for the subsequent release. We demonstrate these operations and discuss the required voltage signals in terms of the forces on the drop. Finally, we discuss possible steps for further improvement in the device.

AB - In two-phase flow microfluidics, there is an increasing interest in technologies which enable the encapsulation of biological cells into aqueous drops and the subsequent study of their molecular (excretion or lysis) products. One not yet available but very promising analysis method is the use of biospecific surface patches embedded in the wall of microfluidic channels. In this paper, we tackle some technological challenges encountered in the development of such applications. In the detection protocol, each drop must be enabled to wet the designated patch, be held in contact long enough for biomolecular detection and subsequently be released. This is engineered via a combination of well-defined chemical sites in the walls of the flow channel and insulated microelectrodes. The tunability of the local electric field allows to modify the competition between chemical (pinning) forces which tend to immobilize the drop and hydrodynamic forces which oppose this process. We developed a prototype microfluidic device which offers this functionality. A channel structure is sandwiched between an actuation surface with electrowetting (EW) electrodes on one side and a detector surface with a hydrophilic patch amidst a hydrophobic environment on the other. Two pairs of carefully aligned EW electrodes are used: one for drop adherence and another one for the subsequent release. We demonstrate these operations and discuss the required voltage signals in terms of the forces on the drop. Finally, we discuss possible steps for further improvement in the device.

KW - IR-102480

KW - METIS-319522

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SN - 1613-4982

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