Electrofusion of cells on chip - From a static, parallel approach to a high-throughput, serial, microdroplet platform

Evelien Kemna

    Research output: ThesisPhD Thesis - Research UT, graduation UT

    26 Downloads (Pure)

    Abstract

    In this thesis, the results of the development of two microfluidic devices (static and high-throughput) for efficient electrofucion of cells were described and evaulated. Electrofusion of cells is an important tool for generating antibody producing hybridomas. The hybridomas are the result of the fusion between a B-cell and and a myeloma cell. The conventional method for generting hybridomas has a very low efficiency (typically around 0.001%). In combination with the increasing need for antibodies, this has motivated us to explore a more efficient method for generating hybridomas. In our opinion, microfluidic chips offer several advantages compared tot the conventional systems
    Original languageUndefined
    Supervisors/Advisors
    • Wolbers, F., Advisor
    • van den Berg, Albert , Supervisor
    Thesis sponsors
    Award date15 Nov 2013
    Place of PublicationZutphen
    Publisher
    Print ISBNs978-90-365-08254
    DOIs
    Publication statusPublished - 15 Nov 2013

    Keywords

    • EWI-24350

    Cite this

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    title = "Electrofusion of cells on chip - From a static, parallel approach to a high-throughput, serial, microdroplet platform",
    abstract = "In this thesis, the results of the development of two microfluidic devices (static and high-throughput) for efficient electrofucion of cells were described and evaulated. Electrofusion of cells is an important tool for generating antibody producing hybridomas. The hybridomas are the result of the fusion between a B-cell and and a myeloma cell. The conventional method for generting hybridomas has a very low efficiency (typically around 0.001{\%}). In combination with the increasing need for antibodies, this has motivated us to explore a more efficient method for generating hybridomas. In our opinion, microfluidic chips offer several advantages compared tot the conventional systems",
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    Electrofusion of cells on chip - From a static, parallel approach to a high-throughput, serial, microdroplet platform. / Kemna, Evelien.

    Zutphen : Wohrmann Print Service, 2013. 182 p.

    Research output: ThesisPhD Thesis - Research UT, graduation UT

    TY - THES

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    AU - Kemna, Evelien

    PY - 2013/11/15

    Y1 - 2013/11/15

    N2 - In this thesis, the results of the development of two microfluidic devices (static and high-throughput) for efficient electrofucion of cells were described and evaulated. Electrofusion of cells is an important tool for generating antibody producing hybridomas. The hybridomas are the result of the fusion between a B-cell and and a myeloma cell. The conventional method for generting hybridomas has a very low efficiency (typically around 0.001%). In combination with the increasing need for antibodies, this has motivated us to explore a more efficient method for generating hybridomas. In our opinion, microfluidic chips offer several advantages compared tot the conventional systems

    AB - In this thesis, the results of the development of two microfluidic devices (static and high-throughput) for efficient electrofucion of cells were described and evaulated. Electrofusion of cells is an important tool for generating antibody producing hybridomas. The hybridomas are the result of the fusion between a B-cell and and a myeloma cell. The conventional method for generting hybridomas has a very low efficiency (typically around 0.001%). In combination with the increasing need for antibodies, this has motivated us to explore a more efficient method for generating hybridomas. In our opinion, microfluidic chips offer several advantages compared tot the conventional systems

    KW - EWI-24350

    U2 - 10.3990/1.9789036508254

    DO - 10.3990/1.9789036508254

    M3 - PhD Thesis - Research UT, graduation UT

    SN - 978-90-365-08254

    PB - Wohrmann Print Service

    CY - Zutphen

    ER -