Electrofusion of cells on chip: from a static, parallel approach to a high-throughput, serial, microdroplet platform

Evelien Kemna

    Research output: ThesisPhD Thesis - Research UT, graduation UT

    34 Downloads (Pure)

    Abstract

    In this thesis, the results of the development of two microfluidic devices (static and high-throughput) for efficient electrofucion of cells were described and evaulated. Electrofusion of cells is an important tool for generating antibody producing hybridomas. The hybridomas are the result of the fusion between a B-cell and and a myeloma cell. The conventional method for generting hybridomas has a very low efficiency (typically around 0.001%). In combination with the increasing need for antibodies, this has motivated us to explore a more efficient method for generating hybridomas. In our opinion, microfluidic chips offer several advantages compared tot the conventional systems
    Original languageEnglish
    Supervisors/Advisors
    • van den Berg, Albert, Supervisor
    • Wolbers, F., Co-Supervisor
    Thesis sponsors
    Award date15 Nov 2013
    Place of PublicationZutphen
    Publisher
    Print ISBNs978-90-365-08254
    DOIs
    Publication statusPublished - 15 Nov 2013

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