Abstract
In this thesis, the results of the development of two microfluidic devices (static and high-throughput) for efficient electrofucion of cells were described and evaulated. Electrofusion of cells is an important tool for generating antibody producing hybridomas. The hybridomas are the result of the fusion between a B-cell and and a myeloma cell. The conventional method for generting hybridomas has a very low efficiency (typically around 0.001%). In combination with the increasing need for antibodies, this has motivated us to explore a more efficient method for generating hybridomas. In our opinion, microfluidic chips offer several advantages compared tot the conventional systems
Original language | English |
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Award date | 15 Nov 2013 |
Place of Publication | Zutphen |
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Print ISBNs | 978-90-365-08254 |
DOIs | |
Publication status | Published - 15 Nov 2013 |