Electrofusion of cells on chip: from a static, parallel approach to a high-throughput, serial, microdroplet platform

Evelien W.M. Kemna

doi:10.3990/1.9789036508254

Electrofusion of cells on chip: from a static, parallel approach to a high-throughput, serial, microdroplet platform

Evelien W.M. Kemna

Research output: Thesis › PhD Thesis - Research UT, graduation UT

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Abstract

In this thesis, the results of the development of two microfluidic devices (static and high-throughput) for efficient electrofucion of cells were described and evaulated. Electrofusion of cells is an important tool for generating antibody producing hybridomas. The hybridomas are the result of the fusion between a B-cell and and a myeloma cell. The conventional method for generting hybridomas has a very low efficiency (typically around 0.001%). In combination with the increasing need for antibodies, this has motivated us to explore a more efficient method for generating hybridomas. In our opinion, microfluidic chips offer several advantages compared tot the conventional systems

Original language	English
Qualification	Doctor of Philosophy
Awarding Institution	University of Twente
Supervisors/Advisors	van den Berg, Albert, Supervisor Wolbers, F., Co-Supervisor
Thesis sponsors	ERC
Award date	15 Nov 2013
Place of Publication	Zutphen
Publisher	Wohrmann Print Service
Print ISBNs	978-90-365-08254
DOIs	https://doi.org/10.3990/1.9789036508254
Publication status	Published - 15 Nov 2013

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PhD thesis E.W.M. KemnaFinal published version, 77.6 MB

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title = "Electrofusion of cells on chip: from a static, parallel approach to a high-throughput, serial, microdroplet platform",

abstract = "In this thesis, the results of the development of two microfluidic devices (static and high-throughput) for efficient electrofucion of cells were described and evaulated. Electrofusion of cells is an important tool for generating antibody producing hybridomas. The hybridomas are the result of the fusion between a B-cell and and a myeloma cell. The conventional method for generting hybridomas has a very low efficiency (typically around 0.001\%). In combination with the increasing need for antibodies, this has motivated us to explore a more efficient method for generating hybridomas. In our opinion, microfluidic chips offer several advantages compared tot the conventional systems",

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T2 - from a static, parallel approach to a high-throughput, serial, microdroplet platform

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N2 - In this thesis, the results of the development of two microfluidic devices (static and high-throughput) for efficient electrofucion of cells were described and evaulated. Electrofusion of cells is an important tool for generating antibody producing hybridomas. The hybridomas are the result of the fusion between a B-cell and and a myeloma cell. The conventional method for generting hybridomas has a very low efficiency (typically around 0.001%). In combination with the increasing need for antibodies, this has motivated us to explore a more efficient method for generating hybridomas. In our opinion, microfluidic chips offer several advantages compared tot the conventional systems

AB - In this thesis, the results of the development of two microfluidic devices (static and high-throughput) for efficient electrofucion of cells were described and evaulated. Electrofusion of cells is an important tool for generating antibody producing hybridomas. The hybridomas are the result of the fusion between a B-cell and and a myeloma cell. The conventional method for generting hybridomas has a very low efficiency (typically around 0.001%). In combination with the increasing need for antibodies, this has motivated us to explore a more efficient method for generating hybridomas. In our opinion, microfluidic chips offer several advantages compared tot the conventional systems

U2 - 10.3990/1.9789036508254

DO - 10.3990/1.9789036508254

M3 - PhD Thesis - Research UT, graduation UT

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PB - Wohrmann Print Service

CY - Zutphen

ER -

Electrofusion of cells on chip: from a static, parallel approach to a high-throughput, serial, microdroplet platform

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