Electrokinetic DNA transport in 20nm-high nanoslits: Evidence for movement through a wall-adsorbed polymer nanogel

O. Castillo; Oscar Castillo-Fernandez; G.B. Beugelaar; Jan William van Nieuwkasteele; Johan G. Bomer; Martin Arundell; Josep Samitier; Albert van den Berg; Jan C.T. Eijkel

doi:10.1002/elps.201100278

Electrokinetic DNA transport in 20nm-high nanoslits: Evidence for movement through a wall-adsorbed polymer nanogel

O. Castillo, Oscar Castillo-Fernandez, G.B. Beugelaar, Jan William van Nieuwkasteele, Johan G. Bomer, Martin Arundell, Josep Samitier, Albert van den Berg, Jan C.T. Eijkel

Research output: Contribution to journal › Article › Academic › peer-review

17 Citations (Scopus)

Abstract

The electrokinetic transport behavior of lambda-DNA (48 kbp) in 20 nm-high fused-silica nanoslits in the presence of short-chain PVP is investigated. Mobility and video data show a number of phenomena that are typical of DNA transport through gels or polymer solutions, thus indicative of rigid migration obstacles in the DNA pathway. Calculations show that a several nanometer thin layer of wall-adsorbed PVP ('nano-gel') can provide such a rigid obstacle matrix to the DNA. Such ultrathin wall-adsorbed polymer layers represent a new type of matrix for electrokinetic DNA separation.

Original language	Undefined
Pages (from-to)	2402-2409
Number of pages	8
Journal	Electrophoresis
Volume	32
Issue number	18
DOIs	https://doi.org/10.1002/elps.201100278
Publication status	Published - Sep 2011

Keywords

λ-DNA
EWI-20879
Nanoslits
PVP
Biased reptation
METIS-281588
IR-78647
Caterpillar movement
Pathways

Access to Document

10.1002/elps.201100278

http://eprints.eemcs.utwente.nl/secure2/20879/01/2402_ftp.pdf

Cite this

Castillo, O. ; Castillo-Fernandez, Oscar ; Beugelaar, G.B. ; van Nieuwkasteele, Jan William ; Bomer, Johan G. ; Arundell, Martin ; Samitier, Josep ; van den Berg, Albert ; Eijkel, Jan C.T. / Electrokinetic DNA transport in 20nm-high nanoslits: Evidence for movement through a wall-adsorbed polymer nanogel. In: Electrophoresis. 2011 ; Vol. 32, No. 18. pp. 2402-2409.

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title = "Electrokinetic DNA transport in 20nm-high nanoslits: Evidence for movement through a wall-adsorbed polymer nanogel",

abstract = "The electrokinetic transport behavior of lambda-DNA (48 kbp) in 20 nm-high fused-silica nanoslits in the presence of short-chain PVP is investigated. Mobility and video data show a number of phenomena that are typical of DNA transport through gels or polymer solutions, thus indicative of rigid migration obstacles in the DNA pathway. Calculations show that a several nanometer thin layer of wall-adsorbed PVP ('nano-gel') can provide such a rigid obstacle matrix to the DNA. Such ultrathin wall-adsorbed polymer layers represent a new type of matrix for electrokinetic DNA separation.",

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author = "O. Castillo and Oscar Castillo-Fernandez and G.B. Beugelaar and {van Nieuwkasteele}, {Jan William} and Bomer, {Johan G.} and Martin Arundell and Josep Samitier and {van den Berg}, Albert and Eijkel, {Jan C.T.}",

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Electrokinetic DNA transport in 20nm-high nanoslits: Evidence for movement through a wall-adsorbed polymer nanogel. / Castillo, O.; Castillo-Fernandez, Oscar; Beugelaar, G.B.; van Nieuwkasteele, Jan William ; Bomer, Johan G.; Arundell, Martin; Samitier, Josep; van den Berg, Albert ; Eijkel, Jan C.T.

In: Electrophoresis, Vol. 32, No. 18, 09.2011, p. 2402-2409.

Research output: Contribution to journal › Article › Academic › peer-review

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AU - Castillo, O.

AU - Castillo-Fernandez, Oscar

AU - Beugelaar, G.B.

AU - van Nieuwkasteele, Jan William

AU - Bomer, Johan G.

AU - Arundell, Martin

AU - Samitier, Josep

AU - van den Berg, Albert

AU - Eijkel, Jan C.T.

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AB - The electrokinetic transport behavior of lambda-DNA (48 kbp) in 20 nm-high fused-silica nanoslits in the presence of short-chain PVP is investigated. Mobility and video data show a number of phenomena that are typical of DNA transport through gels or polymer solutions, thus indicative of rigid migration obstacles in the DNA pathway. Calculations show that a several nanometer thin layer of wall-adsorbed PVP ('nano-gel') can provide such a rigid obstacle matrix to the DNA. Such ultrathin wall-adsorbed polymer layers represent a new type of matrix for electrokinetic DNA separation.

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KW - Biased reptation

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KW - IR-78647

KW - Caterpillar movement

KW - Pathways

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