Electrokinetic DNA transport in 20nm-high nanoslits: Evidence for movement through a wall-adsorbed polymer nanogel

O. Castillo, Oscar Castillo-Fernandez, G.B. Beugelaar, Jan William van Nieuwkasteele, Johan G. Bomer, Martin Arundell, Josep Samitier, Albert van den Berg, Jan C.T. Eijkel

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    16 Citations (Scopus)

    Abstract

    The electrokinetic transport behavior of lambda-DNA (48 kbp) in 20 nm-high fused-silica nanoslits in the presence of short-chain PVP is investigated. Mobility and video data show a number of phenomena that are typical of DNA transport through gels or polymer solutions, thus indicative of rigid migration obstacles in the DNA pathway. Calculations show that a several nanometer thin layer of wall-adsorbed PVP ('nano-gel') can provide such a rigid obstacle matrix to the DNA. Such ultrathin wall-adsorbed polymer layers represent a new type of matrix for electrokinetic DNA separation.
    Original languageUndefined
    Pages (from-to)2402-2409
    Number of pages8
    JournalElectrophoresis
    Volume32
    Issue number18
    DOIs
    Publication statusPublished - Sep 2011

    Keywords

    • λ-DNA
    • EWI-20879
    • Nanoslits
    • PVP
    • Biased reptation
    • METIS-281588
    • IR-78647
    • Caterpillar movement
    • Pathways

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