Electrokinetic DNA transport in 20nm-high nanoslits: Evidence for movement through a wall-adsorbed polymer nanogel

O. Castillo; Oscar Castillo-Fernandez; G.B. Beugelaar; Jan William van Nieuwkasteele; Johan G. Bomer; Martin Arundell; Josep Samitier; Albert van den Berg; Jan C.T. Eijkel

doi:10.1002/elps.201100278

Electrokinetic DNA transport in 20nm-high nanoslits: Evidence for movement through a wall-adsorbed polymer nanogel

O. Castillo, Oscar Castillo-Fernandez, G.B. Beugelaar, Jan William van Nieuwkasteele, Johan G. Bomer, Martin Arundell, Josep Samitier, Albert van den Berg, Jan C.T. Eijkel

Research output: Contribution to journal › Article › Academic › peer-review

21 Citations (Scopus)

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Abstract

The electrokinetic transport behavior of lambda-DNA (48 kbp) in 20 nm-high fused-silica nanoslits in the presence of short-chain PVP is investigated. Mobility and video data show a number of phenomena that are typical of DNA transport through gels or polymer solutions, thus indicative of rigid migration obstacles in the DNA pathway. Calculations show that a several nanometer thin layer of wall-adsorbed PVP ('nano-gel') can provide such a rigid obstacle matrix to the DNA. Such ultrathin wall-adsorbed polymer layers represent a new type of matrix for electrokinetic DNA separation.

Original language	Undefined
Pages (from-to)	2402-2409
Number of pages	8
Journal	Electrophoresis
Volume	32
Issue number	18
DOIs	https://doi.org/10.1002/elps.201100278
Publication status	Published - Sept 2011

Keywords

λ-DNA
EWI-20879
Nanoslits
PVP
Biased reptation
METIS-281588
IR-78647
Caterpillar movement
Pathways

Access to Document

10.1002/elps.201100278

http://eprints.eemcs.utwente.nl/secure2/20879/01/2402_ftp.pdf

Cite this

@article{4d69fc9cf66f42de8022d8f84f1ed9f0,

title = "Electrokinetic DNA transport in 20nm-high nanoslits: Evidence for movement through a wall-adsorbed polymer nanogel",

abstract = "The electrokinetic transport behavior of lambda-DNA (48 kbp) in 20 nm-high fused-silica nanoslits in the presence of short-chain PVP is investigated. Mobility and video data show a number of phenomena that are typical of DNA transport through gels or polymer solutions, thus indicative of rigid migration obstacles in the DNA pathway. Calculations show that a several nanometer thin layer of wall-adsorbed PVP ('nano-gel') can provide such a rigid obstacle matrix to the DNA. Such ultrathin wall-adsorbed polymer layers represent a new type of matrix for electrokinetic DNA separation.",

keywords = "λ-DNA, EWI-20879, Nanoslits, PVP, Biased reptation, METIS-281588, IR-78647, Caterpillar movement, Pathways",

author = "O. Castillo and Oscar Castillo-Fernandez and G.B. Beugelaar and {van Nieuwkasteele}, {Jan William} and Bomer, {Johan G.} and Martin Arundell and Josep Samitier and {van den Berg}, Albert and Eijkel, {Jan C.T.}",

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doi = "10.1002/elps.201100278",

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TY - JOUR

T1 - Electrokinetic DNA transport in 20nm-high nanoslits: Evidence for movement through a wall-adsorbed polymer nanogel

AU - Castillo, O.

AU - Castillo-Fernandez, Oscar

AU - Beugelaar, G.B.

AU - van Nieuwkasteele, Jan William

AU - Bomer, Johan G.

AU - Arundell, Martin

AU - Samitier, Josep

AU - van den Berg, Albert

AU - Eijkel, Jan C.T.

N1 - eemcs-eprint-20879

PY - 2011/9

Y1 - 2011/9

N2 - The electrokinetic transport behavior of lambda-DNA (48 kbp) in 20 nm-high fused-silica nanoslits in the presence of short-chain PVP is investigated. Mobility and video data show a number of phenomena that are typical of DNA transport through gels or polymer solutions, thus indicative of rigid migration obstacles in the DNA pathway. Calculations show that a several nanometer thin layer of wall-adsorbed PVP ('nano-gel') can provide such a rigid obstacle matrix to the DNA. Such ultrathin wall-adsorbed polymer layers represent a new type of matrix for electrokinetic DNA separation.

AB - The electrokinetic transport behavior of lambda-DNA (48 kbp) in 20 nm-high fused-silica nanoslits in the presence of short-chain PVP is investigated. Mobility and video data show a number of phenomena that are typical of DNA transport through gels or polymer solutions, thus indicative of rigid migration obstacles in the DNA pathway. Calculations show that a several nanometer thin layer of wall-adsorbed PVP ('nano-gel') can provide such a rigid obstacle matrix to the DNA. Such ultrathin wall-adsorbed polymer layers represent a new type of matrix for electrokinetic DNA separation.

KW - λ-DNA

KW - EWI-20879

KW - Nanoslits

KW - PVP

KW - Biased reptation

KW - METIS-281588

KW - IR-78647

KW - Caterpillar movement

KW - Pathways

U2 - 10.1002/elps.201100278

DO - 10.1002/elps.201100278

M3 - Article

SN - 0173-0835

VL - 32

SP - 2402

EP - 2409

JO - Electrophoresis

JF - Electrophoresis

IS - 18

ER -