Abstract
The electrokinetic transport behavior of lambda-DNA (48 kbp) in 20 nm-high fused-silica nanoslits in the presence of short-chain PVP is investigated. Mobility and video data show a number of phenomena that are typical of DNA transport through gels or polymer solutions, thus indicative of rigid migration obstacles in the DNA pathway. Calculations show that a several nanometer thin layer of wall-adsorbed PVP ('nano-gel') can provide such a rigid obstacle matrix to the DNA. Such ultrathin wall-adsorbed polymer layers represent a new type of matrix for electrokinetic DNA separation.
Original language | Undefined |
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Pages (from-to) | 2402-2409 |
Number of pages | 8 |
Journal | Electrophoresis |
Volume | 32 |
Issue number | 18 |
DOIs | |
Publication status | Published - Sept 2011 |
Keywords
- λ-DNA
- EWI-20879
- Nanoslits
- PVP
- Biased reptation
- METIS-281588
- IR-78647
- Caterpillar movement
- Pathways