Electrokinetic DNA transport in 20nm-high nanoslits: Evidence for movement through a wall-adsorbed polymer nanogel

O. Castillo, Oscar Castillo-Fernandez, G.B. Beugelaar, Jan William van Nieuwkasteele, Johan G. Bomer, Martin Arundell, Josep Samitier, Albert van den Berg, Jan C.T. Eijkel

Research output: Contribution to journalArticleAcademicpeer-review

14 Citations (Scopus)

Abstract

The electrokinetic transport behavior of lambda-DNA (48 kbp) in 20 nm-high fused-silica nanoslits in the presence of short-chain PVP is investigated. Mobility and video data show a number of phenomena that are typical of DNA transport through gels or polymer solutions, thus indicative of rigid migration obstacles in the DNA pathway. Calculations show that a several nanometer thin layer of wall-adsorbed PVP ('nano-gel') can provide such a rigid obstacle matrix to the DNA. Such ultrathin wall-adsorbed polymer layers represent a new type of matrix for electrokinetic DNA separation.
Original languageUndefined
Pages (from-to)2402-2409
Number of pages8
JournalElectrophoresis
Volume32
Issue number18
DOIs
Publication statusPublished - Sep 2011

Keywords

  • λ-DNA
  • EWI-20879
  • Nanoslits
  • PVP
  • Biased reptation
  • METIS-281588
  • IR-78647
  • Caterpillar movement
  • Pathways

Cite this

@article{4d69fc9cf66f42de8022d8f84f1ed9f0,
title = "Electrokinetic DNA transport in 20nm-high nanoslits: Evidence for movement through a wall-adsorbed polymer nanogel",
abstract = "The electrokinetic transport behavior of lambda-DNA (48 kbp) in 20 nm-high fused-silica nanoslits in the presence of short-chain PVP is investigated. Mobility and video data show a number of phenomena that are typical of DNA transport through gels or polymer solutions, thus indicative of rigid migration obstacles in the DNA pathway. Calculations show that a several nanometer thin layer of wall-adsorbed PVP ('nano-gel') can provide such a rigid obstacle matrix to the DNA. Such ultrathin wall-adsorbed polymer layers represent a new type of matrix for electrokinetic DNA separation.",
keywords = "λ-DNA, EWI-20879, Nanoslits, PVP, Biased reptation, METIS-281588, IR-78647, Caterpillar movement, Pathways",
author = "O. Castillo and Oscar Castillo-Fernandez and G.B. Beugelaar and {van Nieuwkasteele}, {Jan William} and Bomer, {Johan G.} and Martin Arundell and Josep Samitier and {van den Berg}, Albert and Eijkel, {Jan C.T.}",
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year = "2011",
month = "9",
doi = "10.1002/elps.201100278",
language = "Undefined",
volume = "32",
pages = "2402--2409",
journal = "Electrophoresis",
issn = "0173-0835",
publisher = "Wiley-VCH Verlag",
number = "18",

}

Electrokinetic DNA transport in 20nm-high nanoslits: Evidence for movement through a wall-adsorbed polymer nanogel. / Castillo, O.; Castillo-Fernandez, Oscar; Beugelaar, G.B.; van Nieuwkasteele, Jan William; Bomer, Johan G.; Arundell, Martin; Samitier, Josep; van den Berg, Albert; Eijkel, Jan C.T.

In: Electrophoresis, Vol. 32, No. 18, 09.2011, p. 2402-2409.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Electrokinetic DNA transport in 20nm-high nanoslits: Evidence for movement through a wall-adsorbed polymer nanogel

AU - Castillo, O.

AU - Castillo-Fernandez, Oscar

AU - Beugelaar, G.B.

AU - van Nieuwkasteele, Jan William

AU - Bomer, Johan G.

AU - Arundell, Martin

AU - Samitier, Josep

AU - van den Berg, Albert

AU - Eijkel, Jan C.T.

N1 - eemcs-eprint-20879

PY - 2011/9

Y1 - 2011/9

N2 - The electrokinetic transport behavior of lambda-DNA (48 kbp) in 20 nm-high fused-silica nanoslits in the presence of short-chain PVP is investigated. Mobility and video data show a number of phenomena that are typical of DNA transport through gels or polymer solutions, thus indicative of rigid migration obstacles in the DNA pathway. Calculations show that a several nanometer thin layer of wall-adsorbed PVP ('nano-gel') can provide such a rigid obstacle matrix to the DNA. Such ultrathin wall-adsorbed polymer layers represent a new type of matrix for electrokinetic DNA separation.

AB - The electrokinetic transport behavior of lambda-DNA (48 kbp) in 20 nm-high fused-silica nanoslits in the presence of short-chain PVP is investigated. Mobility and video data show a number of phenomena that are typical of DNA transport through gels or polymer solutions, thus indicative of rigid migration obstacles in the DNA pathway. Calculations show that a several nanometer thin layer of wall-adsorbed PVP ('nano-gel') can provide such a rigid obstacle matrix to the DNA. Such ultrathin wall-adsorbed polymer layers represent a new type of matrix for electrokinetic DNA separation.

KW - λ-DNA

KW - EWI-20879

KW - Nanoslits

KW - PVP

KW - Biased reptation

KW - METIS-281588

KW - IR-78647

KW - Caterpillar movement

KW - Pathways

U2 - 10.1002/elps.201100278

DO - 10.1002/elps.201100278

M3 - Article

VL - 32

SP - 2402

EP - 2409

JO - Electrophoresis

JF - Electrophoresis

SN - 0173-0835

IS - 18

ER -