Abstract
| Original language | Undefined |
|---|---|
| Pages (from-to) | 2402-2409 |
| Number of pages | 8 |
| Journal | Electrophoresis |
| Volume | 32 |
| Issue number | 18 |
| DOIs | |
| Publication status | Published - Sep 2011 |
Keywords
- λ-DNA
- EWI-20879
- Nanoslits
- PVP
- Biased reptation
- METIS-281588
- IR-78647
- Caterpillar movement
- Pathways
Cite this
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Electrokinetic DNA transport in 20nm-high nanoslits: Evidence for movement through a wall-adsorbed polymer nanogel. / Castillo, O.; Castillo-Fernandez, Oscar; Beugelaar, G.B.; van Nieuwkasteele, Jan William; Bomer, Johan G.; Arundell, Martin; Samitier, Josep; van den Berg, Albert; Eijkel, Jan C.T.
In: Electrophoresis, Vol. 32, No. 18, 09.2011, p. 2402-2409.Research output: Contribution to journal › Article › Academic › peer-review
TY - JOUR
T1 - Electrokinetic DNA transport in 20nm-high nanoslits: Evidence for movement through a wall-adsorbed polymer nanogel
AU - Castillo, O.
AU - Castillo-Fernandez, Oscar
AU - Beugelaar, G.B.
AU - van Nieuwkasteele, Jan William
AU - Bomer, Johan G.
AU - Arundell, Martin
AU - Samitier, Josep
AU - van den Berg, Albert
AU - Eijkel, Jan C.T.
N1 - eemcs-eprint-20879
PY - 2011/9
Y1 - 2011/9
N2 - The electrokinetic transport behavior of lambda-DNA (48 kbp) in 20 nm-high fused-silica nanoslits in the presence of short-chain PVP is investigated. Mobility and video data show a number of phenomena that are typical of DNA transport through gels or polymer solutions, thus indicative of rigid migration obstacles in the DNA pathway. Calculations show that a several nanometer thin layer of wall-adsorbed PVP ('nano-gel') can provide such a rigid obstacle matrix to the DNA. Such ultrathin wall-adsorbed polymer layers represent a new type of matrix for electrokinetic DNA separation.
AB - The electrokinetic transport behavior of lambda-DNA (48 kbp) in 20 nm-high fused-silica nanoslits in the presence of short-chain PVP is investigated. Mobility and video data show a number of phenomena that are typical of DNA transport through gels or polymer solutions, thus indicative of rigid migration obstacles in the DNA pathway. Calculations show that a several nanometer thin layer of wall-adsorbed PVP ('nano-gel') can provide such a rigid obstacle matrix to the DNA. Such ultrathin wall-adsorbed polymer layers represent a new type of matrix for electrokinetic DNA separation.
KW - λ-DNA
KW - EWI-20879
KW - Nanoslits
KW - PVP
KW - Biased reptation
KW - METIS-281588
KW - IR-78647
KW - Caterpillar movement
KW - Pathways
U2 - 10.1002/elps.201100278
DO - 10.1002/elps.201100278
M3 - Article
VL - 32
SP - 2402
EP - 2409
JO - Electrophoresis
JF - Electrophoresis
SN - 0173-0835
IS - 18
ER -