Electrokinetic DNA transport in 20nm-high nanoslits: Evidence for movement through a wall-adsorbed polymer nanogel

O. Castillo, Oscar Castillo-Fernandez, G.B. Beugelaar, Jan William van Nieuwkasteele, Johan G. Bomer, Martin Arundell, Josep Samitier, Albert van den Berg, Jan C.T. Eijkel

    Research output: Contribution to journalArticleAcademicpeer-review

    16 Citations (Scopus)

    Abstract

    The electrokinetic transport behavior of lambda-DNA (48 kbp) in 20 nm-high fused-silica nanoslits in the presence of short-chain PVP is investigated. Mobility and video data show a number of phenomena that are typical of DNA transport through gels or polymer solutions, thus indicative of rigid migration obstacles in the DNA pathway. Calculations show that a several nanometer thin layer of wall-adsorbed PVP ('nano-gel') can provide such a rigid obstacle matrix to the DNA. Such ultrathin wall-adsorbed polymer layers represent a new type of matrix for electrokinetic DNA separation.
    Original languageUndefined
    Pages (from-to)2402-2409
    Number of pages8
    JournalElectrophoresis
    Volume32
    Issue number18
    DOIs
    Publication statusPublished - Sep 2011

    Keywords

    • λ-DNA
    • EWI-20879
    • Nanoslits
    • PVP
    • Biased reptation
    • METIS-281588
    • IR-78647
    • Caterpillar movement
    • Pathways

    Cite this

    @article{4d69fc9cf66f42de8022d8f84f1ed9f0,
    title = "Electrokinetic DNA transport in 20nm-high nanoslits: Evidence for movement through a wall-adsorbed polymer nanogel",
    abstract = "The electrokinetic transport behavior of lambda-DNA (48 kbp) in 20 nm-high fused-silica nanoslits in the presence of short-chain PVP is investigated. Mobility and video data show a number of phenomena that are typical of DNA transport through gels or polymer solutions, thus indicative of rigid migration obstacles in the DNA pathway. Calculations show that a several nanometer thin layer of wall-adsorbed PVP ('nano-gel') can provide such a rigid obstacle matrix to the DNA. Such ultrathin wall-adsorbed polymer layers represent a new type of matrix for electrokinetic DNA separation.",
    keywords = "λ-DNA, EWI-20879, Nanoslits, PVP, Biased reptation, METIS-281588, IR-78647, Caterpillar movement, Pathways",
    author = "O. Castillo and Oscar Castillo-Fernandez and G.B. Beugelaar and {van Nieuwkasteele}, {Jan William} and Bomer, {Johan G.} and Martin Arundell and Josep Samitier and {van den Berg}, Albert and Eijkel, {Jan C.T.}",
    note = "eemcs-eprint-20879",
    year = "2011",
    month = "9",
    doi = "10.1002/elps.201100278",
    language = "Undefined",
    volume = "32",
    pages = "2402--2409",
    journal = "Electrophoresis",
    issn = "0173-0835",
    publisher = "Wiley-VCH Verlag",
    number = "18",

    }

    Electrokinetic DNA transport in 20nm-high nanoslits: Evidence for movement through a wall-adsorbed polymer nanogel. / Castillo, O.; Castillo-Fernandez, Oscar; Beugelaar, G.B.; van Nieuwkasteele, Jan William; Bomer, Johan G.; Arundell, Martin; Samitier, Josep; van den Berg, Albert; Eijkel, Jan C.T.

    In: Electrophoresis, Vol. 32, No. 18, 09.2011, p. 2402-2409.

    Research output: Contribution to journalArticleAcademicpeer-review

    TY - JOUR

    T1 - Electrokinetic DNA transport in 20nm-high nanoslits: Evidence for movement through a wall-adsorbed polymer nanogel

    AU - Castillo, O.

    AU - Castillo-Fernandez, Oscar

    AU - Beugelaar, G.B.

    AU - van Nieuwkasteele, Jan William

    AU - Bomer, Johan G.

    AU - Arundell, Martin

    AU - Samitier, Josep

    AU - van den Berg, Albert

    AU - Eijkel, Jan C.T.

    N1 - eemcs-eprint-20879

    PY - 2011/9

    Y1 - 2011/9

    N2 - The electrokinetic transport behavior of lambda-DNA (48 kbp) in 20 nm-high fused-silica nanoslits in the presence of short-chain PVP is investigated. Mobility and video data show a number of phenomena that are typical of DNA transport through gels or polymer solutions, thus indicative of rigid migration obstacles in the DNA pathway. Calculations show that a several nanometer thin layer of wall-adsorbed PVP ('nano-gel') can provide such a rigid obstacle matrix to the DNA. Such ultrathin wall-adsorbed polymer layers represent a new type of matrix for electrokinetic DNA separation.

    AB - The electrokinetic transport behavior of lambda-DNA (48 kbp) in 20 nm-high fused-silica nanoslits in the presence of short-chain PVP is investigated. Mobility and video data show a number of phenomena that are typical of DNA transport through gels or polymer solutions, thus indicative of rigid migration obstacles in the DNA pathway. Calculations show that a several nanometer thin layer of wall-adsorbed PVP ('nano-gel') can provide such a rigid obstacle matrix to the DNA. Such ultrathin wall-adsorbed polymer layers represent a new type of matrix for electrokinetic DNA separation.

    KW - λ-DNA

    KW - EWI-20879

    KW - Nanoslits

    KW - PVP

    KW - Biased reptation

    KW - METIS-281588

    KW - IR-78647

    KW - Caterpillar movement

    KW - Pathways

    U2 - 10.1002/elps.201100278

    DO - 10.1002/elps.201100278

    M3 - Article

    VL - 32

    SP - 2402

    EP - 2409

    JO - Electrophoresis

    JF - Electrophoresis

    SN - 0173-0835

    IS - 18

    ER -