The electrokinetic transport behavior of lambda-DNA (48 kbp) in 20 nm-high fused-silica nanoslits in the presence of short-chain PVP is investigated. Mobility and video data show a number of phenomena that are typical of DNA transport through gels or polymer solutions, thus indicative of rigid migration obstacles in the DNA pathway. Calculations show that a several nanometer thin layer of wall-adsorbed PVP ('nano-gel') can provide such a rigid obstacle matrix to the DNA. Such ultrathin wall-adsorbed polymer layers represent a new type of matrix for electrokinetic DNA separation.
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Castillo, O., Castillo-Fernandez, O., Beugelaar, G. B., van Nieuwkasteele, J. W., Bomer, J. G., Arundell, M., ... Eijkel, J. C. T. (2011). Electrokinetic DNA transport in 20nm-high nanoslits: Evidence for movement through a wall-adsorbed polymer nanogel. Electrophoresis, 32(18), 2402-2409. https://doi.org/10.1002/elps.201100278