Electrokinetic label-free screening chip: a marriage of multiplexing and high throughput analysis using surface plasmon resonance imaging

Research output: Contribution to journalArticleAcademicpeer-review

34 Citations (Scopus)

Abstract

We present an electrokinetic label-free biomolecular screening chip (Glass/PDMS) to screen up to 10 samples simultaneously using surface plasmon resonance imaging (iSPR). This approach reduces the duration of an experiment when compared to conventional experimental methods. This new device offers a high degree of parallelization not only for analyte samples, but also for multiplex analyte interactions where up to 90 ligands are immobilized on the sensing surface. The proof of concept has been demonstrated with well-known biomolecular interactant pairs. The new chip can be used for high throughput screening applications and kinetics parameter extraction, simultaneously, of interactant-protein complex formation
Original languageUndefined
Article number10.1039/c000705f
Pages (from-to)986-990
Number of pages5
JournalLab on a chip
Volume10
Issue number8
DOIs
Publication statusPublished - 2010

Keywords

  • EWI-18133
  • IR-72394
  • METIS-269018

Cite this

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title = "Electrokinetic label-free screening chip: a marriage of multiplexing and high throughput analysis using surface plasmon resonance imaging",
abstract = "We present an electrokinetic label-free biomolecular screening chip (Glass/PDMS) to screen up to 10 samples simultaneously using surface plasmon resonance imaging (iSPR). This approach reduces the duration of an experiment when compared to conventional experimental methods. This new device offers a high degree of parallelization not only for analyte samples, but also for multiplex analyte interactions where up to 90 ligands are immobilized on the sensing surface. The proof of concept has been demonstrated with well-known biomolecular interactant pairs. The new chip can be used for high throughput screening applications and kinetics parameter extraction, simultaneously, of interactant-protein complex formation",
keywords = "EWI-18133, IR-72394, METIS-269018",
author = "G. Krishnamoorthy and Edwin Carlen and Bomer, {Johan G.} and Dani{\"e}l Wijnperle and {de Boer}, {Hans L.} and {van den Berg}, Albert and Schasfoort, {Richardus B.M.}",
year = "2010",
doi = "10.1039/c000705f",
language = "Undefined",
volume = "10",
pages = "986--990",
journal = "Lab on a chip",
issn = "1473-0197",
publisher = "Royal Society of Chemistry",
number = "8",

}

Electrokinetic label-free screening chip: a marriage of multiplexing and high throughput analysis using surface plasmon resonance imaging. / Krishnamoorthy, G.; Carlen, Edwin; Bomer, Johan G.; Wijnperle, Daniël; de Boer, Hans L.; van den Berg, Albert; Schasfoort, Richardus B.M.

In: Lab on a chip, Vol. 10, No. 8, 10.1039/c000705f, 2010, p. 986-990.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Electrokinetic label-free screening chip: a marriage of multiplexing and high throughput analysis using surface plasmon resonance imaging

AU - Krishnamoorthy, G.

AU - Carlen, Edwin

AU - Bomer, Johan G.

AU - Wijnperle, Daniël

AU - de Boer, Hans L.

AU - van den Berg, Albert

AU - Schasfoort, Richardus B.M.

PY - 2010

Y1 - 2010

N2 - We present an electrokinetic label-free biomolecular screening chip (Glass/PDMS) to screen up to 10 samples simultaneously using surface plasmon resonance imaging (iSPR). This approach reduces the duration of an experiment when compared to conventional experimental methods. This new device offers a high degree of parallelization not only for analyte samples, but also for multiplex analyte interactions where up to 90 ligands are immobilized on the sensing surface. The proof of concept has been demonstrated with well-known biomolecular interactant pairs. The new chip can be used for high throughput screening applications and kinetics parameter extraction, simultaneously, of interactant-protein complex formation

AB - We present an electrokinetic label-free biomolecular screening chip (Glass/PDMS) to screen up to 10 samples simultaneously using surface plasmon resonance imaging (iSPR). This approach reduces the duration of an experiment when compared to conventional experimental methods. This new device offers a high degree of parallelization not only for analyte samples, but also for multiplex analyte interactions where up to 90 ligands are immobilized on the sensing surface. The proof of concept has been demonstrated with well-known biomolecular interactant pairs. The new chip can be used for high throughput screening applications and kinetics parameter extraction, simultaneously, of interactant-protein complex formation

KW - EWI-18133

KW - IR-72394

KW - METIS-269018

U2 - 10.1039/c000705f

DO - 10.1039/c000705f

M3 - Article

VL - 10

SP - 986

EP - 990

JO - Lab on a chip

JF - Lab on a chip

SN - 1473-0197

IS - 8

M1 - 10.1039/c000705f

ER -